Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,723,685	A→G	9.2%	intergenic (+15/+15)	PP_3288 → / ← PP_3289	universal stress protein family protein/acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,723,685	0	A	G	9.2%	179.1 / 6.2	65	intergenic (+15/+15)	PP_3288/PP_3289	universal stress protein family protein/acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (32/27);  new base G (3/3);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGTCATGGGCAGTTATCACCACCGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTGGAGACCATGCA  >  NC_002947/3723547‑3723828                                                                                                                                           |                                                                                                                                                  aCTGGTCATGGGCAGTTATCACCACCGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCTTGCGGGCCTTGt                                                                                                                                         >  5:278057/1‑149 (MQ=255)  cTGGTCATGGGCAGTTATCACCACCGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCTTGCGGGCCTTGt                                                                                                                                         <  4:301286/148‑1 (MQ=255)     gTCATGGGCAGTTATCACCACCGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCttt                                                                                                                                     >  5:165608/1‑149 (MQ=255)     gTCATGGGCAGTTATCACCACCGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCttt                                                                                                                                     <  8:24632/149‑1 (MQ=255)      tCATGGGCAGTTATCACCACCGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCTTGCGGGCCTTGtgcttc                                                                                                                                    <  6:278057/149‑6 (MQ=255)      tCATGGGCAGTTATCACCACCGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCtttt                                                                                                                                    >  5:110070/1‑149 (MQ=255)           ggCAGTTATCACCACCGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAg                                                                                                                                <  1:154470/148‑1 (MQ=255)                       caccGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAg                                                                                                                  >  2:259105/1‑149 (MQ=255)                          cGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTc                                                                                                               <  7:134886/149‑1 (MQ=255)                            tGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTTTGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGt                                                                                                             <  1:136749/149‑1 (MQ=255)                            tGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGt                                                                                                             <  2:22128/149‑1 (MQ=255)                            tGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGt                                                                                                             <  2:259698/149‑1 (MQ=255)                              gCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTg                                                                                                            <  3:187416/148‑1 (MQ=255)                                   ggCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGaa                                                                                                       >  2:147525/1‑148 (MQ=255)                                                gCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAg                                                                                                         >  5:7700/1‑133 (MQ=255)                                                gCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAg                                                                                                         <  6:7700/133‑1 (MQ=255)                                                       tGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑g                                                                                                                        >  1:122987/1‑111 (MQ=255)                                                       tGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑g                                                                                                                        <  2:122987/111‑1 (MQ=255)                                                       tGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGt                                                                                                             >  7:58409/1‑122 (MQ=255)                                                       tGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGt                                                                                                             <  8:58409/122‑1 (MQ=255)                                                         ccGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGtt                                                                                                                <  2:220428/117‑1 (MQ=255)                                                         ccGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGtt                                                                                                                >  1:220428/1‑117 (MQ=255)                                                           gAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCgg‑gg                                                                               >  3:134323/1‑147 (MQ=255)                                                              cgcgTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCg                                                                          >  3:356324/1‑149 (MQ=255)                                                               gcgTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCgg                                                                         >  3:372959/1‑149 (MQ=255)                                                                   tATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGt                                                                                                             >  8:219490/1‑110 (MQ=255)                                                                   tATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGt                                                                                                             <  7:219490/110‑1 (MQ=255)                                                                    aTGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATATCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGt                                                                    >  8:350055/1‑149 (MQ=255)                                                                       cATCGGTTGTCCAGCAGTGTTCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAACGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGa                                                                  <  7:285625/148‑1 (MQ=255)                                                                        aTCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAg                                                                >  8:299779/1‑149 (MQ=255)                                                                            gTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCACAAGGGCCTTGTCTTTTCAAGGCCGCa                                                                                                                          <  7:99914/89‑1 (MQ=255)                                                                            gTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCACAAGGGCCTTGTCTTTTCAAGGCCGCa                                                                                                                          >  8:99914/1‑89 (MQ=255)                                                                                  cagcagTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCg                                                                                                              >  6:396499/1‑94 (MQ=255)                                                                                  cagcagTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCg                                                                                                              <  5:396499/94‑1 (MQ=255)                                                                                  cagcagTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGcc                                                       <  1:259105/148‑1 (MQ=255)                                                                                   agcagTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCtt                                                     <  7:299779/149‑1 (MQ=255)                                                                                             tGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAAtctc                                                                                                   >  2:91352/1‑94 (MQ=255)                                                                                             tGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGaa                                                                 <  2:133547/127‑1 (MQ=255)                                                                                               gTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGGTGGTAGCGGGAGGTGAAGCCCAGTGCCtt                                                     <  1:25195/138‑1 (MQ=255)                                                                                               gTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCtt                                                     >  2:25195/1‑137 (MQ=255)                                                                                                  aTTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCAt                                      >  8:133724/1‑149 (MQ=255)                                                                                                   tttCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCAt                                      <  1:147525/148‑1 (MQ=255)                                                                                                    ttCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATcg                                    >  2:332120/1‑149 (MQ=255)                                                                                                         cAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTa                                <  7:133724/148‑1 (MQ=255)                                                                                                               gcTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTTACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTg                         <  1:332120/149‑1 (MQ=255)                                                                                                                 tCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGc                       >  6:100283/1‑149 (MQ=255)                                                                                                                 tCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGc                       >  3:129347/1‑149 (MQ=255)                                                                                                                 tCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGc                       >  6:6514/1‑149 (MQ=255)                                                                                                                  cccAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGc                       >  3:178553/1‑148 (MQ=255)                                                                                                                   ccAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTg                                                    <  1:256424/118‑1 (MQ=255)                                                                                                                   ccAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTg                                                    >  2:256424/1‑118 (MQ=255)                                                                                                                   ccAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGcc                      <  4:372959/148‑1 (MQ=255)                                                                                                                   ccAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGc                       >  8:153281/1‑147 (MQ=255)                                                                                                                      aGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGt                  <  6:110070/149‑1 (MQ=255)                                                                                                                          tGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCt              >  1:275817/1‑149 (MQ=255)                                                                                                                            aaagacaAGGCCCTTGCGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTgg            >  7:364417/5‑149 (MQ=255)                                                                                                                            aaGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTg             >  7:14488/1‑148 (MQ=255)                                                                                                                              gCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCTTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTg             <  3:250812/146‑1 (MQ=255)                                                                                                                              gCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTg             >  4:250812/1‑146 (MQ=255)                                                                                                                               gacaAGGCCCTTGCGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGt                  <  2:168467/139‑1 (MQ=255)                                                                                                                               gacaAGGCCCTTGCGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGt                  >  1:168467/2‑140 (MQ=255)                                                                                                                                acaAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGAc                                                  <  6:334273/107‑1 (MQ=255)                                                                                                                                acaAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGAc                                                  >  5:334273/1‑107 (MQ=255)                                                                                                                                  aaGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTGGAGACCa      >  4:322658/1‑149 (MQ=255)                                                                                                                                       ccGCAAGGGCCTTGTCTTTTCAAGGCCGCAGGGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTGGAGACCATGCa  >  1:257078/1‑149 (MQ=255)                                                                                                                                           aaGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTg             >  6:254457/1‑133 (MQ=255)                                                                                                                                           aaGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTg             <  5:254457/133‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                  ACTGGTCATGGGCAGTTATCACCACCGTGGCATCGGCTGGTTCATCGGCAGTACTGCCGAGCGCGTTATGCATCGGTTGTCCAGCAGTGTGCTGGTGATTTCTCCAGAGCGCTCCCAAGGCTGAAGCACAAGGCCCGCAAGGGCCTTGTCTTTTCAAGGCCGCA‑GGTCAAGTTCGTGCAGAATCTCGCTCACATCGCCGCCGG‑GGTAGCGGGAGGTGAAGCCCAGTGCCTTGACCAGCCGGTGCATCGCGTAGTTGCTGGCCAGGTCCCTGGAGACCATGCA  >  NC_002947/3723547‑3723828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑