| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,727,128 | T→A | 7.6% | L114Q (CTG→CAG) | PP_3292 → | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,727,128 | 0 | T | A | 7.6% | 143.8 / 3.5 | 53 | L114Q (CTG→CAG) | PP_3292 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (24/25); new base A (2/2); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01 | |||||||||||
GGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGGCGCAG > NC_002947/3726982‑3727276 | ggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCGGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCctgctg < 1:78494/148‑1 (MQ=255)ggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCctgctg < 1:215382/148‑1 (MQ=255)ggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTgg < 3:332526/149‑1 (MQ=255) gCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTggg > 2:121385/1‑149 (MQ=255) aGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAgg > 3:321310/1‑148 (MQ=255) cGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCAc < 5:120950/149‑1 (MQ=255) ggCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCGGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaac < 7:170540/149‑4 (MQ=255) gTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtg < 4:299130/149‑1 (MQ=255) tGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGc > 3:313151/1‑140 (MQ=255) tGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGc < 4:313151/140‑1 (MQ=255) ccGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAggg > 5:153829/1‑149 (MQ=255) cGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGgc > 5:238249/1‑149 (MQ=255) aGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCAc > 2:80964/1‑123 (MQ=255) aGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCAc < 1:80964/123‑1 (MQ=255) ccATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACg < 2:264648/149‑1 (MQ=255) ccATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCGGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACg < 4:26702/149‑1 (MQ=255) ccAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGc < 6:347184/147‑1 (MQ=255) aCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaac < 5:402941/104‑4 (MQ=37) aCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaac > 6:402941/1‑101 (MQ=37) aGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAgg > 4:164123/1‑149 (MQ=255) aGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAgg < 5:155827/149‑1 (MQ=255) gCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGc > 5:2285/1‑148 (MQ=255) ggcctgCCAGCCTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGaa > 4:95235/6‑136 (MQ=255) ggcctgCCAGCCTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGaa < 3:95235/131‑1 (MQ=255) ggCAGGCCAGACTGGCGTTCGTTCGCTCTGTGGCTCGGTAATCGTACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCGGTGCTGGGCTCAGGCAGGTTTTGGGTGGCAGGGCGCTCAGTGACGAAGTTCGTGGCGATCTTCGAGGCCAAc < 6:47307/149‑1 (MQ=255) aGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTg < 4:351458/116‑1 (MQ=255) ggcaccaCTGGCGTTCGTTCGCCCCGTGGCCCGGCAAGCCACCATCCTCCGGGTGGGGCTGTGGTGCCTG‑CCCCTGCTGGCCACTGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGc < 1:121385/143‑1 (MQ=255) gCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTggg < 4:10956/80‑1 (MQ=255) gCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTggg > 3:10956/1‑80 (MQ=255) aGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCTCAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGc < 6:2285/149‑1 (MQ=255) aCTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCt > 3:54313/1‑148 (MQ=255) tGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCaa > 5:157048/1‑149 (MQ=255) gTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTgg < 4:321310/149‑1 (MQ=255) gTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTg > 7:174489/1‑148 (MQ=255) ttCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTg < 4:372254/131‑1 (MQ=255) ttCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTg > 3:372254/1‑131 (MQ=255) cGCTCAGTGGCTCTGGAATGGAACATCCTGCTGGTGGTGCTGTTGTGGCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCTCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATAc < 7:385986/148‑1 (MQ=255) gCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATAccc > 8:272237/1‑141 (MQ=255) gCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATAccc < 7:272237/141‑1 (MQ=255) tCGGGAATCGAACATGCTGCTGGTGGTGCTGTTTTGCCTG‑GCCCTGATGGGCACAGGCACCTTTTGGCTGGCAGGGCGATAAGTGACGCAGATCGTGGCGAACTGAGAGGCCAACCTGCAGGCAAAATTGGATAAACACGAAGATGaa > 6:292418/1‑148 (MQ=255) tCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGaa > 6:11238/1‑148 (MQ=255) ccTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAg > 4:229082/1‑113 (MQ=255) ccTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAg < 3:229082/113‑1 (MQ=255) gctgGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATAc > 8:185560/1‑119 (MQ=255) gctgGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATAc < 7:185560/119‑1 (MQ=255) ttgtGCCTG‑GCCCTGCTTGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGgtgt < 6:238249/148‑1 (MQ=255) ttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGtgtg < 6:157048/149‑1 (MQ=255) ttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAAGGACGCAGATCGTGGCGAACTGCGAGGGCCACCGGGAGGGTGAAGTGGATATACCCGAAGCTGAAAAACTCACCGCGCTTCTGGGCAAACCGGtgtg > 1:101190/1‑149 (MQ=255) gtgCCTG‑GGCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACATGCATGCTCATGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGc < 5:292418/148‑1 (MQ=255) cctgtgCCCAGCAGGGC‑CAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACCGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGt > 8:317104/6‑149 (MQ=255) ccTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGt < 7:376951/149‑1 (MQ=255) cTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTg > 4:67761/1‑149 (MQ=255) g‑gCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAACCTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGcc > 8:263007/1‑149 (MQ=255) gCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCt > 1:268060/1‑149 (MQ=255) ccTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTgg > 4:354340/1‑149 (MQ=255) ctgGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGGCGCa < 3:164123/149‑1 (MQ=255) tgGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGGCGCAg > 1:402782/1‑149 (MQ=255) | GGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGGCGCAG > NC_002947/3726982‑3727276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |