| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,741,959 | A→T | 9.3% | intergenic (‑27/+386) | PP_3305 ← / ← PP_3306 | TerC family membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,741,959 | 0 | A | T | 9.3% | 153.0 / 6.0 | 54 | intergenic (‑27/+386) | PP_3305/PP_3306 | TerC family membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (24/25); new base T (2/3); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01 | |||||||||||
GCGGTGGAGCACACCTAAGTCAAGCGCCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCTTGCAGTGAAAAACTAAC > NC_002947/3741813‑3742107 | gcgGTGGAGCACACCTAAGTCAAGCGCCAACAGTGTTAGCACAACAGCGAAAATGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAGCGTCTTGGTAGAGATACGGACGAAGGGCAGACATGCGCCCCGCCT‑GTATAGTCGTCGTTGag > 8:381619/1‑147 (MQ=255) gCACACCTAAGTCAAGCGCCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCATAGTTGTTCAACTACGa < 3:179842/149‑1 (MQ=255) acCTAAGTCAAGCGCCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTcc < 4:127001/149‑1 (MQ=255) aGTCAAGCGCCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGAc < 8:287572/147‑1 (MQ=255) aaGCGCCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTTGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCg > 2:219919/1‑149 (MQ=255) aaGCGCCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGc < 6:394681/148‑1 (MQ=255) aaGCGCCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCg > 7:151408/1‑149 (MQ=255) aGCGCCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGCTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGATCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTAAGACATGGCgg > 5:407099/1‑149 (MQ=255) cgcCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGAGGta > 8:293577/1‑149 (MQ=255) cacaACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAgggg > 7:304253/1‑149 (MQ=255) acaGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCg < 2:105674/149‑1 (MQ=255) acaGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCg > 2:165429/1‑149 (MQ=255) aGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGaacaa < 8:11181/106‑1 (MQ=255) aGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGaacaa > 7:11181/1‑106 (MQ=255) gAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGt < 8:151408/148‑1 (MQ=255) aGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGaa < 2:406440/149‑1 (MQ=255) aagccaaagccaGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATaaa < 3:305646/149‑1 (MQ=255) agccaaagccaGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTc < 3:406854/139‑1 (MQ=255) agccaaagccaGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTc > 4:406854/1‑139 (MQ=255) ccaaagccaGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGaa < 8:304253/149‑1 (MQ=255) caaagccaGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACAGTCAGAGGGTCCCGGCGTCACGAATAAAGAAt > 6:257411/1‑149 (MQ=255) aaagccaGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTc < 2:235421/135‑1 (MQ=255) aaagccaGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTc > 1:235421/1‑135 (MQ=255) aaagccaGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCATAGTTGTTCAACTACGACtatac > 5:195662/1‑97 (MQ=38) aaagccaGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCATAGTTGTTCAACTACGACtatac < 6:195662/101‑5 (MQ=38) caGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCTACATGGCGGTATAACCGTCAGAGGGGCCCGGAGTCACGAATAAACAATTTATc > 5:349756/1‑148 (MQ=255) aGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAg > 7:164144/1‑149 (MQ=255) aaGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCt > 8:112310/1‑149 (MQ=255) gAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTg > 6:176768/1‑148 (MQ=255) gAACGTCTTCGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGc < 1:219919/149‑1 (MQ=255) tCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTa < 1:165429/149‑1 (MQ=255) tCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTa > 8:274642/1‑149 (MQ=255) ttGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGa < 7:112310/149‑1 (MQ=255) ttGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTACGACATGGCGGTATAACCGTCAGAGGGGCACGGCGTAACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAg > 7:337445/1‑148 (MQ=255) ttGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCATAGTTGTTCAACTACGACt < 1:237261/69‑1 (MQ=37) ttGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCATAGTTGTTCAACTACGACt > 2:237261/1‑69 (MQ=37) tAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAAc < 5:176768/149‑1 (MQ=255) agaTACGTACTAAGGGCAGACATGAGCCCCTCCTAGTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCagag > 1:263084/1‑89 (MQ=255) agaTACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCagag < 2:263084/88‑1 (MQ=255) gTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACaaa > 7:37547/1‑149 (MQ=255) aCTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATc > 8:30133/1‑149 (MQ=255) cTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGAGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATc < 7:293577/148‑1 (MQ=255) aGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACTAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGt < 5:257411/149‑1 (MQ=255) cAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTAAGACTCCGACATGGCTGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCAt < 3:186/149‑1 (MQ=255) cgagaTGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCAt < 5:361861/144‑1 (MQ=255) aCATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATc < 4:82884/138‑1 (MQ=255) aCATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATc > 3:82884/1‑138 (MQ=255) gAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGa > 4:336981/1‑119 (MQ=255) gAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGa < 3:336981/119‑1 (MQ=255) ccccTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTa > 7:219269/1‑148 (MQ=255) ct‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCt > 3:146506/1‑149 (MQ=255) tataGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCTTg < 2:406078/148‑1 (MQ=255) tataGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCTTg < 8:164144/148‑1 (MQ=255) taGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCAAGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCTTGCAg < 3:49283/149‑1 (MQ=255) tCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCTTGCAGTg > 5:11229/1‑148 (MQ=255) gTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCTTGCAGTGaaaaa < 7:274642/148‑1 (MQ=255) gAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCTTGCAGTGAAAaacta > 7:257661/1‑148 (MQ=255) aacaacTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAAt > 4:184247/1‑107 (MQ=255) aacaacTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAAt < 3:184247/107‑1 (MQ=255) aacaacTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCTTGCAGTGAAAaactaac < 3:304606/149‑1 (MQ=255) | GCGGTGGAGCACACCTAAGTCAAGCGCCAACAGTGTTAGCACAACAGCGAAAAAGGAAAGCCAAAGCCAGAGGTCAGTACCAAGAACGTCTTGGTAGAGATACGTACTAAGGGCAGACATGAGCCCCTCCT‑GTATAGTCGTAGTTGAACAACTATGACTCCGACATGGCGGTATAACCGTCAGAGGGGCCCGGCGTCACGAATAAAGAATTTATCGAGCGCGAGTAGGCTGCAAGTAGAAAACATTTACAAATCTTGTCCATGACAAGCTAAAACGCTTGCAGTGAAAAACTAAC > NC_002947/3741813‑3742107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |