Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,812,662	T→A	8.5%	I329N (ATT→AAT)	PP_3368 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,812,662	0	T	A	8.5%	229.0 / 10.2	82	I329N (ATT→AAT)	PP_3368	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (37/38);  new base A (4/3);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-01

CGTGGGCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGAC  >  NC_002947/3812516‑3812803                                                                                                                                                   |                                                                                                                                              cGTGGGCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTg                                                                                                                                             >  3:351188/1‑149 (MQ=255)           gTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGGCAATGTCCATGAGgc                                                                                                                                   <  2:327625/149‑1 (MQ=255)           gTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGGCAATGTCCATGAGgc                                                                                                                                   <  4:253784/149‑1 (MQ=255)             aCTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGgcgc                                                                                                                                 >  3:313303/1‑149 (MQ=255)              cTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGgcgc                                                                                                                                 <  3:164934/148‑1 (MQ=255)                 aTGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCt                                                                                                                               <  3:165264/147‑1 (MQ=255)                 aTGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTa                                                                                                                              <  1:300927/148‑1 (MQ=255)                 aTGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTa                                                                                                                              <  4:351188/148‑1 (MQ=255)                      gtcggtcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAg                                                                                                                                     <  3:261275/136‑1 (MQ=255)                      gtcggtcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTgg                                                                                                                        >  8:222461/1‑149 (MQ=255)                      gtcggtcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTCCCCATGAg                                                                                                                                     >  4:261275/1‑136 (MQ=255)                          gtcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATg                                                                                                                                       >  4:101606/1‑130 (MQ=255)                          gtcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATg                                                                                                                                       <  3:101606/130‑1 (MQ=255)                           tcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAgtg                                                                                                                   >  6:123165/1‑147 (MQ=255)                           tcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAgcg                                                                                                                   >  4:50740/1‑149 (MQ=255)                           tcgCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAgcg                                                                                                                   >  2:402209/1‑149 (MQ=255)                             gcggTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAgcggc                                                                                                                 <  8:315726/146‑1 (MQ=255)                                 tgctgcTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTcc                                                                                                             <  6:386217/149‑1 (MQ=255)                                         ttCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCGTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCAt                                                                                                                                        <  2:148373/114‑1 (MQ=255)                                         ttCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCAt                                                                                                                                        >  1:148373/1‑114 (MQ=255)                                           cTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgcgg                                                                                                   >  6:380293/1‑149 (MQ=255)                                            tttACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgcggc                                                                                                  >  4:155943/1‑149 (MQ=255)                                            tttACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgcggc                                                                                                  >  5:415047/1‑149 (MQ=255)                                              tACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATgcgg                                                                                                   <  6:250678/146‑1 (MQ=255)                                                  ttCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCaa                                                                                            >  1:371579/1‑149 (MQ=255)                                                     gccgccCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCACATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCt                                                                                         >  3:30319/1‑149 (MQ=255)                                                        gccCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCa                                                                                                                                         <  8:63672/98‑1 (MQ=255)                                                        gccCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCa                                                                                                                                         >  7:63672/1‑98 (MQ=255)                                                        gccCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGc                                                                                      <  6:415047/149‑1 (MQ=255)                                                        gccCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGc                                                                                      <  4:313303/149‑1 (MQ=255)                                                           cAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTAAAGATCCGCCTCATGGACATTACCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCaa                                                                                   >  1:333681/1‑149 (MQ=255)                                                                gcTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGccc                                                                              >  2:114860/1‑149 (MQ=255)                                                                gcTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGccc                                                                              <  1:402209/149‑1 (MQ=255)                                                                  tgtgGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAgc                                                                                                                    <  2:244232/109‑1 (MQ=255)                                                                  tgtgGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAgc                                                                                                                    >  1:244232/1‑109 (MQ=255)                                                                      gAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTgggg                                                                         <  7:222461/148‑1 (MQ=255)                                                                                ctgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTgg                                                               <  3:155943/148‑1 (MQ=255)                                                                                ctgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTg                                                                <  1:114860/147‑1 (MQ=255)                                                                                 tgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACGTGGCCAATGCCCTGGGGCCGt                                                                     >  5:370763/1‑141 (MQ=255)                                                                                 tgGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGt                                                                     <  6:370763/141‑1 (MQ=255)                                                                                    gCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGc                                                          <  3:50740/149‑1 (MQ=255)                                                                                     cATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGGCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTg                                                                <  8:368362/142‑1 (MQ=255)                                                                                     cATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTg                                                                >  7:368362/1‑142 (MQ=255)                                                                                         ggCCTGGTGGGCACCATGATCGCCTTGGCGGAACCGCTACAGATCCGCATCATGGACATTGCCCATGAGGCGCCTAGCCAGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATgg                                                      <  3:4853/148‑1 (MQ=255)                                                                                          gCCTGGTGGGCACCTTGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGc                                                                                      <  4:332224/115‑1 (MQ=255)                                                                                            cTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGAt                                                  <  7:18351/149‑1 (MQ=255)                                                                                             tggtggGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGAt                                                  >  6:255205/1‑148 (MQ=255)                                                                                              ggtggGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCa                                                >  8:21063/1‑149 (MQ=255)                                                                                               gtggGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCa                                                <  5:255205/148‑1 (MQ=255)                                                                                                tggGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACt                                              <  7:21063/149‑1 (MQ=255)                                                                                                 gggCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCt                                                                                         <  2:320284/105‑1 (MQ=255)                                                                                                 gggCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCt                                                                                         >  1:320284/1‑105 (MQ=255)                                                                                                    cACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGTGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCg                                          <  5:123165/149‑1 (MQ=255)                                                                                                           aTCGCCTTGGCTGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGAGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCAttg                                    <  6:254695/148‑3 (MQ=255)                                                                                                           aTCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCaa                                                                                            <  8:199391/92‑1 (MQ=255)                                                                                                           aTCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCaa                                                                                            >  7:199391/1‑92 (MQ=255)                                                                                                            tCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAg                                                            >  7:355065/1‑123 (MQ=255)                                                                                                            tCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAg                                                            <  8:355065/123‑1 (MQ=255)                                                                                                              ggcTTGGCGTCACCGGTACAGATCCGCCTGATGGACATTTCCCATGAGGCGCCTAGCCTGGGAGCGGGGTCCAACCATGCGGCGTTCAACCTGGGGTATGCGCTGTGGCCGTGGCTGGGAGGGTTGGCGATCACTGCCGGGATGGGCTg                                <  4:30319/147‑1 (MQ=255)                                                                                                                 ttGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATg                                                       >  1:194860/1‑123 (MQ=255)                                                                                                                 ttGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATg                                                       <  2:194860/123‑1 (MQ=255)                                                                                                                 ttGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGAc                             >  6:330499/1‑149 (MQ=255)                                                                                                                   ggcggcACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGAcc                            <  2:222798/148‑1 (MQ=255)                                                                                                                    gcggcACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAg                          >  7:232026/1‑149 (MQ=255)                                                                                                                         aCCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCTCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATggg                                   <  1:193785/135‑1 (MQ=255)                                                                                                                         aCCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATggg                                   >  2:193785/1‑135 (MQ=255)                                                                                                                         aCCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCg                     >  7:78853/1‑149 (MQ=255)                                                                                                                              tACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCgg                    <  2:16491/145‑1 (MQ=255)                                                                                                                              tACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCgg                    >  1:16491/1‑145 (MQ=255)                                                                                                                              tACAGATCCGCCTCATGGACATTGCCAATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCCACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATa                 >  4:341893/1‑148 (MQ=255)                                                                                                                                cAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACAt              <  7:110042/149‑1 (MQ=255)                                                                                                                                   aTCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATgg                                                      <  3:103075/106‑1 (MQ=255)                                                                                                                                   aTCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATgg                                                      >  4:103075/1‑106 (MQ=255)                                                                                                                                        ccTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgccg      >  6:328073/1‑149 (MQ=255)                                                                                                                                         cTCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgccgc     >  7:209035/1‑149 (MQ=255)                                                                                                                                         cTCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgccgc     >  4:170879/1‑149 (MQ=255)                                                                                                                                         cTCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgccgc     >  1:330117/1‑149 (MQ=255)                                                                                                                                          tCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGa                                                             >  2:75307/1‑92 (MQ=255)                                                                                                                                          tCATGGGCAATGTCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGa                                                             <  1:75307/92‑1 (MQ=255)                                                                                                                                          tCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGcgccgc     >  6:90130/1‑148 (MQ=255)                                                                                                                                           cATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGa   >  2:114513/1‑149 (MQ=255)                                                                                                                                            aTGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGAc  >  4:92809/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                              CGTGGGCTTGGTACTGATGTGGTCGGTCGCCGTGCTGCTGTTCTTTACCTTCGCCGCCCAGGCGCTGTGGAGCCTGCTGCTGGGCATCGGCCTGGTGGGCACCATGATCGCCTTGGCGGCACCGCTACAGATCCGCCTCATGGACATTGCCCATGAGGCGCCTAGCCTGGCAGCGGCGTCCAACCATGCGGCGTTCAACCTGGCCAATGCCCTGGGGCCGTGGCTGGGAGGCATGGCGATCACTGCCGGCATGGGCTGGACCAGCACCGGATACATCGGCGCCGCGAC  >  NC_002947/3812516‑3812803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑