Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 432,993 A→T 8.0% intergenic (+18/+29) PP_0355 → / ← glcB two‑component system response regulator/malate synthase G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947432,9930AT 8.0% 219.6 / 8.2 75intergenic (+18/+29)PP_0355/glcBtwo‑component system response regulator/malate synthase G
Reads supporting (aligned to +/- strand):  ref base A (33/36);  new base T (4/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

TGTCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGC  >  NC_002947/432848‑433135
                                                                                                                                                 |                                                                                                                                              
tgtCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTaaaa                                                                                                                                             <  5:158737/149‑1 (MQ=255)
 gtCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAAc                                                                                                                                            >  1:27609/1‑149 (MQ=255)
  tCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACg                                                                                                                                           <  7:318744/149‑1 (MQ=255)
     tgCGACACCGATGAGGAAGATGCATTGTATGTGATCCACCTGCGGCTTGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTc                                                                                                                                        <  4:313814/148‑1 (MQ=255)
           acCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTc                                                                                                                                        <  8:206768/143‑1 (MQ=255)
           acCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTc                                                                                                                                        >  7:206768/1‑143 (MQ=255)
            ccGATGTTGAAGAGGCAGTGGATGTTATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCTGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTgg                                                                                                                                 <  2:71305/149‑1 (MQ=255)
             cGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTgg                                                                                                                                 >  1:184167/1‑148 (MQ=255)
             cGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCATGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCACGGGGACCGTAAACCGGTCACCCTgg                                                                                                                                 >  6:326246/1‑148 (MQ=255)
               aTGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGgcag                                                                                                                              <  2:29945/149‑1 (MQ=255)
                    gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTCCCCCTg                                                                                                                                  >  5:158187/1‑140 (MQ=255)
                    gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTCCCCCTg                                                                                                                                  <  6:158187/140‑1 (MQ=255)
                     aaGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAtg                                                                                                                        <  6:348458/149‑1 (MQ=255)
                          gCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATgctgct                                                                                                                   >  8:222110/1‑149 (MQ=255)
                          gCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGGAAAACGGTCCCCCAGGCAGCAGAAtctgct                                                                                                                   >  2:402490/1‑149 (MQ=255)
                            aGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTa                                                                                                                 <  1:402490/149‑1 (MQ=255)
                            aGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTACCCCTGGCAGCAGATGCTGCTTa                                                                                                                 >  7:325237/1‑149 (MQ=255)
                             gTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCGCAAGCCGGTGGATCAGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTAc                                                                                                                <  1:303993/149‑1 (MQ=255)
                              tgtaTGTGATGCACGTGGGGGTGGTGGACTTCCTGCTCAAGCCGGGGGATCTGGGCAAGCTGTTGGAGCTGGTTAACAAATAACTTAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTa                                                                                                                 <  2:184167/144‑1 (MQ=255)
                              tGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTAc                                                                                                                >  1:108285/1‑148 (MQ=255)
                              tGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACa                                                                                                               <  5:222/149‑1 (MQ=255)
                                   gtgATGCAGCTGGGGGTGGTGGACTTCCTTCTCAAGCCGGTGGATCTGTGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCGTGGCAGCAGATGCTGCTTACAAccc                                                                                                           <  8:325237/148‑1 (MQ=255)
                                   gtgATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCg                                                                                                          >  7:85907/1‑149 (MQ=255)
                                    ggttggaCCGGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGTATCTGGGCAAGCTGTGGGAGCTGGTTAACAACGAATTGAAAATTGGATGATCCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAcc                                                                                                            <  6:378286/140‑1 (MQ=255)
                                        gCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcg                                                                                                     >  2:305400/1‑149 (MQ=255)
                                         cACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcgc                                                                                                    >  2:268992/1‑149 (MQ=255)
                                          aCCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcgcg                                                                                                   >  8:81414/1‑149 (MQ=255)
                                           ccTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACgcgc                                                                                                  <  8:81377/149‑1 (MQ=255)
                                             tGGGGGTTGTTGACTTCCTGCTCAAGCCTGTGGATCTGGGCAAGCTGTTGGAGCTGGTTAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAtgctg                                                                                                                     <  4:230137/128‑1 (MQ=255)
                                             tGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAtgctg                                                                                                                     >  3:230137/1‑128 (MQ=255)
                                               ggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTg                                                                                              <  7:81414/149‑1 (MQ=255)
                                                 ggtggtGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGtt                                                                                                        <  1:327020/137‑1 (MQ=255)
                                                 ggtggtGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGtt                                                                                                        >  2:327020/1‑137 (MQ=255)
                                                 ggtggtGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGaa                                                                                            <  2:27609/149‑1 (MQ=255)
                                                   tggtggACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACt                                                                                          >  8:149412/1‑149 (MQ=255)
                                                      tggACTTCCTGCACAAGCCGGTGGATCTCGGCAAGCTGATGCAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGCGCACCCTAAAACGGTCCCCCTGGCAGCAGATCCTGCTAACAACCCTTTACGCCCCATGAACTccc                                                                                       >  4:360235/1‑147 (MQ=255)
                                                        taCTTCCTGCTCAAGCCGGTGGATCTGGGAAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGAGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGTTTACAACCCGTTACGCGCCTTGAACTCgcgg                                                                                     <  5:326246/148‑1 (MQ=255)
                                                        gACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgcg                                                                                      <  1:305400/148‑1 (MQ=255)
                                                             ccTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGa                                                                                  <  1:268992/147‑1 (MQ=255)
                                                                  tCAAGCCGTTGTATCTTGGCAAGTTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGCATGATGCGCCAGGGGTTCCGTAAATCGGTCCCCGTGGCAGCAGATGCTGCTTACAACCCGTTAcgc                                                                                                    <  8:343572/124‑1 (MQ=255)
                                                                  tCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAATTTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcgc                                                                                                    <  8:343571/124‑1 (MQ=255)
                                                                  tCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTAcgc                                                                                                    >  7:343571/1‑124 (MQ=255)
                                                                         ggTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTAc                                                                    <  7:222110/149‑1 (MQ=255)
                                                                                cTGGGCAAGCTGTTGGAGCTGGTGAACAAAGATCTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCg                                                             >  6:130090/1‑149 (MQ=255)
                                                                                  gggCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCt                                                               >  3:104444/1‑145 (MQ=255)
                                                                                  gggCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCt                                                               <  4:104444/145‑1 (MQ=255)
                                                                                   ggCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGta                                                          >  7:24280/1‑149 (MQ=255)
                                                                                   ggCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGt                                                           <  7:149412/148‑1 (MQ=255)
                                                                                          tGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGt                                                   >  7:339487/1‑149 (MQ=255)
                                                                                           gTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGtt                                                  >  1:334985/1‑149 (MQ=255)
                                                                                           gTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGtt                                                  >  8:67180/1‑149 (MQ=255)
                                                                                            ttGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTc                                                 >  2:283722/1‑149 (MQ=255)
                                                                                                aGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGtt                                             <  8:85907/149‑1 (MQ=255)
                                                                                                aGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGtt                                             <  2:108285/149‑1 (MQ=255)
                                                                                                   tGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGCCAGCATATGATGCTTACAACCCTTTACGCGCCTTCAACTCGCGGCGACGGCGTTGAAGTACCGGCTCGGTATAACCGTTCGGttgct                                          >  3:182910/1‑149 (MQ=255)
                                                                                                     gTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgcttg                                        >  4:38820/1‑149 (MQ=255)
                                                                                                        aaCAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGcc                                      <  3:38820/148‑1 (MQ=255)
                                                                                                          cAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgc                                           >  4:112306/1‑141 (MQ=255)
                                                                                                          cAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgc                                           <  3:112306/141‑1 (MQ=255)
                                                                                                          cAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCAcc                                   >  4:324495/1‑149 (MQ=255)
                                                                                                           aaaGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATaa                                                       >  3:190609/1‑128 (MQ=255)
                                                                                                           aaaGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATaa                                                       <  4:190609/128‑1 (MQ=255)
                                                                                                           aaaGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCt                                  >  5:345493/1‑149 (MQ=255)
                                                                                                             aGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTc                                <  8:339487/149‑1 (MQ=255)
                                                                                                                     aaaTTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTAcc                         <  3:324495/148‑1 (MQ=255)
                                                                                                                     aaaTTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCa                        <  1:283722/149‑1 (MQ=255)
                                                                                                                      aaTTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTAc                          >  1:69815/1‑146 (MQ=255)
                                                                                                                      aaTTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTAc                          <  2:69815/146‑1 (MQ=255)
                                                                                                                          ggATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTc                    <  6:345493/148‑1 (MQ=255)
                                                                                                                                    ccAGGGGGACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCt         >  2:195072/1‑149 (MQ=255)
                                                                                                                                       gggggACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGta                                                          >  8:269744/1‑97 (MQ=255)
                                                                                                                                       gggggACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGta                                                          <  7:269744/97‑1 (MQ=255)
                                                                                                                                        ggggACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGaa     >  6:104083/1‑149 (MQ=255)
                                                                                                                                           gACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCcaca                <  6:95196/135‑1 (MQ=255)
                                                                                                                                           gACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCcaca                >  5:95196/1‑135 (MQ=255)
                                                                                                                                           gACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGc  >  8:118922/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TGTCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGC  >  NC_002947/432848‑433135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑