Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,908,416	A→G	6.9%	N122S (AAC→AGC)	PP_3450 →	TPR repeat‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,908,416	0	A	G	6.9%	162.0 / 2.1	58	N122S (AAC→AGC)	PP_3450	TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (29/25);  new base G (2/2);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGGCCAGTTGCCTGCCCAGGACTGGCAACGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAG  >  NC_002947/3908276‑3908563                                                                                                                                              |                                                                                                                                                    cacaGGCCAGTTGCCTGCCCAGGACTGGCAACGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGGCGCGGTTGGCCATGGCCAGCcgcgtct                                                                                                                                             <  8:305254/149‑4 (MQ=255)         aGTTGCCTGCCCAGGACTGGCAACGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTc                                                                                                                                     <  3:362619/149‑1 (MQ=255)                    cAGGACTGGCAACGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGccaccac                                                                                                                            >  2:46446/1‑147 (MQ=255)                      ggACTGGCAACGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACcag                                                                                                                         >  1:410704/1‑148 (MQ=255)                         cTGGCAACGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCAtt                                                                                                                                           >  2:224645/1‑127 (MQ=255)                         cTGGCAACGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCAtt                                                                                                                                           <  1:224645/127‑1 (MQ=255)                            gCAACGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCGACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGTTGGCCATGGCCAGCcgcg                                                                                                                                                >  4:1013/1‑119 (MQ=255)                            gCAACGCCTGGCCAATCCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGTTGGCCATGGCCAGCcgcg                                                                                                                                                <  3:1013/119‑1 (MQ=255)                              aaCGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGCACTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGccc                                                                                                                  >  1:84660/1‑148 (MQ=255)                               aCGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAAc                                                                                                               <  3:81385/149‑1 (MQ=255)                                      gCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACgctggct                                                                                                        <  2:410704/149‑1 (MQ=255)                                      gCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACgctgg                                                                                                          <  1:46446/147‑1 (MQ=255)                                      gCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACgctgg                                                                                                          <  6:332029/147‑1 (MQ=255)                                        cAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACgctggc                                                                                                         <  5:244750/146‑1 (MQ=255)                                        cAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACgctggc                                                                                                         >  6:244750/1‑146 (MQ=255)                                         aaTGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACgctggctg                                                                                                       >  5:318683/1‑147 (MQ=255)                                            gCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTc                                                                                                  >  2:360787/1‑149 (MQ=255)                                             ccTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTAGCCTTGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTc                                                                                                  <  2:371720/148‑1 (MQ=255)                                               tataTCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGc                                                                                                >  5:387790/1‑148 (MQ=255)                                                    ccAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGcc                                                                                                                   >  6:10883/1‑124 (MQ=255)                                                    ccAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGcc                                                                                                                   <  5:10883/124‑1 (MQ=255)                                                     cAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGcc                                                                                                                                 <  8:326093/109‑1 (MQ=255)                                                     cAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGcc                                                                                                                                 >  7:326093/1‑109 (MQ=255)                                                     cAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGcc                                                                                         <  6:387790/149‑1 (MQ=255)                                                       gCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTg                                                                                       <  1:360787/149‑1 (MQ=255)                                                               acgaAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTg                                                                               >  8:182/1‑149 (MQ=255)                                                                 gaAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGCTCAACTgcc                                                                             >  3:81100/1‑148 (MQ=255)                                                                         c‑cTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTgca                                                                             >  6:168883/1‑141 (MQ=255)                                                                         c‑cTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTgca                                                                             <  5:168883/141‑1 (MQ=255)                                                                              aCTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGagc                                                                  <  5:171901/148‑1 (MQ=255)                                                                                       ctgGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGc                                                         <  6:318683/148‑1 (MQ=255)                                                                                          gCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACCCTCGCTGCAGTCGCTACCGCCAGATCAACTGCAGCAACCCGAACAGCGCCAGCAACt                                                     >  2:193679/1‑149 (MQ=255)                                                                                            ccACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACttt                                                   >  6:336811/1‑149 (MQ=255)                                                                                            ccACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACttt                                                   <  2:72469/149‑1 (MQ=255)                                                                                             cACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTg                                                  >  4:317215/1‑149 (MQ=255)                                                                                             cACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTg                                                  <  2:84660/149‑1 (MQ=255)                                                                                              aCGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTgg                                                 <  2:221595/149‑1 (MQ=255)                                                                                                     cTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTgca                                                                             <  3:195684/114‑1 (MQ=255)                                                                                                     cTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTgca                                                                             >  4:195684/1‑114 (MQ=255)                                                                                                        ccccGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAAc                                                                        >  4:6022/1‑116 (MQ=255)                                                                                                          ccGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCcgc                                     >  7:379340/1‑149 (MQ=255)                                                                                                          ccGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCcgc                                     >  5:2508/1‑149 (MQ=255)                                                                                                            gCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGcc                                                                                                                                              >  4:15165/1‑42 (MQ=255)                                                                                                            gCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGcc                                                                                                                                              <  3:15165/42‑1 (MQ=255)                                                                                                                  gCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGcga                             <  7:182/149‑1 (MQ=255)                                                                                                                    cAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACgctg                                                                                                           <  5:211068/69‑1 (MQ=255)                                                                                                                    cAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGACGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGcgac                            >  1:268961/1‑148 (MQ=255)                                                                                                                    cAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGACGTCGGCCACCACCAGCAGGCCCAACgctg                                                                                                           >  6:211068/1‑69 (MQ=255)                                                                                                                       ccAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGGAACTGGCCCGCGAAGGCGACGATg                        >  7:281978/1‑149 (MQ=255)                                                                                                                            cgcgGTTGGCCATGGCCAGCCGCGTCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCAGCGAAGGCGACGATGCAgcg                   >  4:68850/1‑149 (MQ=255)                                                                                                                             gcgGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGc                       >  5:357210/1‑144 (MQ=255)                                                                                                                             gcgGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGc                       <  6:357210/144‑1 (MQ=255)                                                                                                                                gCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTgg               >  4:340057/1‑149 (MQ=255)                                                                                                                                  tggccatggccaACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTgg                                                 >  4:287729/1‑113 (MQ=255)                                                                                                                                  tggccatggccaACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTgg                                                 <  3:287729/113‑1 (MQ=255)                                                                                                                                     ccatggccaACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTgcgg          >  1:236726/1‑149 (MQ=255)                                                                                                                                           ccaACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTgcggcgagat    >  3:59635/1‑145 (MQ=255)                                                                                                                                           ccaACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTgcggc         <  4:59635/144‑1 (MQ=255)                                                                                                                                             aaCCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCt     >  4:343513/1‑146 (MQ=255)                                                                                                                                             aaCCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGa   <  3:343513/148‑1 (MQ=255)                                                                                                                                             aaCCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAg  >  3:151605/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                    CACAGGCCAGTTGCCTGCCCAGGACTGGCAACGCCTGGCCAATGCCTATATCCAGCAAGTCGACGAAAAGGGC‑CTGACTGCGCTGCTGGCCCACGCCCCCTCCCCCGCAGCGGCCAACCAGACGCGGCTGGCCATGGCCAACCGCGCCATTGCCGTCGGCCACCACCAGCAGGCCCAACGCTGGCTGCAGTCGCTACCGCCTGATCAACTGCAGCAACCCGAGCAGCGCCAGCAACTTTGGCAACTGGCCCGCGAAGGCGACGATGCAGCGCTGGTGCGGCGCCTGAG  >  NC_002947/3908276‑3908563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑