| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,914,433 | G→C | 6.9% | R118R (CGC→CGG) | PP_3454 ← | transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,914,433 | 0 | G | C | 6.9% | 186.9 / 3.7 | 58 | R118R (CGC→CGG) | PP_3454 | transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (27/27); new base C (2/2); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01 | |||||||||||
GCACCACCAGCAGGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTCCT > NC_002947/3914291‑3914581 | gCACCACCAGCAGGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCgcagc < 8:29746/149‑1 (MQ=255) ccaccaGCAGGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCagcag‑c > 4:278745/1‑149 (MQ=255) ccaccaGCAGGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCagcag‑c < 2:348875/149‑1 (MQ=255) gTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTgcg < 8:279863/144‑1 (MQ=255) gTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTgcg > 7:279863/1‑144 (MQ=255) gTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCAccc < 2:175319/149‑1 (MQ=255) aCTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGcc > 5:400109/1‑149 (MQ=255) aCCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCcagc < 4:63028/148‑1 (MQ=255) aCCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCcag > 1:130544/1‑147 (MQ=255) gACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCAcg < 3:278745/147‑1 (MQ=255) aGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTgg > 7:286847/1‑149 (MQ=255) cTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCAcgc > 6:17417/1‑143 (MQ=255) cTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCAcgc < 5:17417/143‑1 (MQ=255) ctcGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGtt < 5:361709/149‑1 (MQ=255) ctcGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGt < 3:160481/148‑1 (MQ=255) ctcGCCGCGCCAACTCACCGTGCGCTCGGCCAGGGCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGtt < 7:174371/149‑1 (MQ=255) gccgcGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTgg > 2:116971/1‑149 (MQ=255) gccgcGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTg < 6:400109/148‑1 (MQ=255) gccgcGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTg < 2:130544/148‑1 (MQ=255) cgcCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCAc < 8:286847/149‑1 (MQ=255) cAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACGCTGCTGCGCCgcagca < 3:320575/97‑1 (MQ=255) cAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACGCTGCTGCGCCgcagca > 4:320575/1‑97 (MQ=255) tCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGTGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAgtcg > 3:189650/1‑149 (MQ=255) cGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTcaca > 1:163523/1‑126 (MQ=255) cGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCgg > 1:150208/1‑149 (MQ=255) gTGCGCTCGGCCAGGTCGATACGCAGGCCCCCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACCGGGTGTGGGCACGTAGTCGTCGGc > 7:276373/1‑149 (MQ=255) tGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCcag < 8:386774/106‑1 (MQ=255) tGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCcag > 7:386774/1‑106 (MQ=255) gcTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGcccc > 7:100521/1‑148 (MQ=255) tCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCACGGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCAcc < 1:84905/94‑1 (MQ=255) tCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCAcc > 2:84905/1‑93 (MQ=255) ggCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCAtt < 2:150208/148‑1 (MQ=255) ccAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCca < 7:235451/96‑1 (MQ=255) ccAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCca > 8:235451/1‑96 (MQ=255) cGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGcc > 1:100955/1‑148 (MQ=255) aTACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAg > 3:47640/1‑149 (MQ=255) tACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGCTCGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAg < 1:116971/149‑1 (MQ=255) aCGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCa > 1:334168/1‑149 (MQ=255) gCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCAcc > 2:56675/1‑149 (MQ=255) gCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCAcc > 6:55700/1‑149 (MQ=255) gCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGt > 2:342081/1‑149 (MQ=255) caggatcaggTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTg > 2:286567/1‑97 (MQ=255) caggatcaggGCGTTGTCCA‑GGCGCGGTTAGTTGACACTGCTGCTGCGCAGCAGTCGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTg < 1:286567/98‑1 (MQ=255) tcaggTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAgg < 8:326778/122‑1 (MQ=255) tcaggTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAgg > 7:326778/1‑122 (MQ=255) ggTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCgg > 6:115706/1‑149 (MQ=255) cA‑GGCGCGGTTCGTTGACACTGCTGCGGAGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGc < 8:297632/148‑1 (MQ=255) gcgGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAgg < 1:56675/149‑1 (MQ=255) gACACTGCGGCGGCGCAGCAG‑CGTGCGCACCCGTGCCAGC‑AGCACGCGGGGCTCCCAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAgg < 8:276373/148‑1 (MQ=255) accctgctgCGGCGCAGCAG‑CGTGCGCCCCCGGGCCAGC‑AGCACGCGTGGCTCCCAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGCTTGGCAGGGt > 7:262350/4‑149 (MQ=255) acTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCt > 2:264517/1‑149 (MQ=255) ctgctgCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTg < 2:334168/149‑1 (MQ=255) ctgctgCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTg < 1:264517/149‑1 (MQ=255) gctgcGCCGCAGCAG‑TGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGt > 2:192076/1‑60 (MQ=39) gctgcGCCGCAGCAG‑TGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGt < 1:192076/60‑1 (MQ=39) ctgcGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGAtt < 1:395768/135‑1 (MQ=255) ctgcGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGAtt > 2:395768/1‑135 (MQ=255) ctgcGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGc < 4:47640/147‑1 (MQ=255) ggcgCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGc > 3:264274/1‑134 (MQ=255) ggcgCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGc < 4:264274/134‑1 (MQ=255) gcgcAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTCCt > 7:24704/1‑149 (MQ=255) | GCACCACCAGCAGGTTGTACTCACCGCTGGACAGCTCCACCTCCTCGCCGCGCCAACTCACCGTGCGCTCGGCCAGGTCGATACGCAGGCCACCAATCAGGATCAGGTCGTTGTCCA‑GGCGCGGTTCGTTGACACTGCTGCGGCGCAGCAG‑CGTGCGCACCCGGGCCAGC‑AGCACGCGTGGCTCACAGGGTTTGGTCACGTAGTCGTCGGCCCCCATTTCCAGGCCTAGCACCTGGTCATGGCTATCGTCGCGGGCCGTCAGCATGAGGATTGGCAGGGTCTGCGACTCCT > NC_002947/3914291‑3914581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |