| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,018,015 | G→C | 6.1% | Q150H (CAG→CAC) | PP_3545 → | two‑component system sensor histidine kinase/response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,018,015 | 0 | G | C | 6.1% | 197.2 / 2.1 | 61 | Q150H (CAG→CAC) | PP_3545 | two‑component system sensor histidine kinase/response regulator |
| Reads supporting (aligned to +/- strand): ref base G (30/27); new base C (2/2); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATACTGGCAGGGGAAGCGTTCATCGACACCGTCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGG > NC_002947/4017872‑4018160 | aTACTGGCAGGCGAAGCGTTCATCGACACCGTCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGt > 4:103933/1‑149 (MQ=255) cTGGCAGGGGAAGCGTTCATCGACACCGTCACCTTCGGGCTGTACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCGCTGCGTGat < 3:292193/149‑2 (MQ=255) gCAGGGGAAGCGTTCATCGACACCGTCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGAcc < 5:12760/147‑1 (MQ=255) gCAGGGGAAGCGTTCATCGACACCGTCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCg > 1:208649/1‑148 (MQ=255) aGCGTTCATCGACACCGTCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAAc < 3:90268/149‑1 (MQ=255) aGCGTTCATCGACACCGTCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAAc < 2:250495/149‑1 (MQ=255) gTTCATCGACACCGTCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGcc > 6:174455/1‑149 (MQ=255) acCGTCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGc > 8:284026/1‑149 (MQ=255) tCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCgaa < 2:208649/149‑1 (MQ=255) gggCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCGTATGCTCCGCAGCGAATTCCGGGCGCCTACCTGTTCGCCTATGACATCACGCACCGCGTCACCGAACAGGAACGCCTGCACCAGCCCGAAGAAGCAc > 1:102226/1‑149 (MQ=255) gacgacGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑cc < 5:174455/148‑1 (MQ=255) gacgCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCGCTGCGTGatg < 5:152384/105‑3 (MQ=255) gacgCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCGCTGCGTGatg > 6:152384/1‑103 (MQ=255) cgCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGgcgc < 7:284026/149‑1 (MQ=255) accacGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑AAGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGAGTGACCGAACAgg > 1:257444/1‑107 (MQ=255) tACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGc > 4:226117/1‑149 (MQ=255) tACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGc > 1:220791/1‑149 (MQ=255) gccctgaattcgctgctgagcatcgagccacgggtggtagcgaattCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAgg > 5:78917‑M2/47‑149 (MQ=255) ccctgaattcgctgctgagcatcgagccacgggtggtagcgaattCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGc > 3:90933‑M2/46‑149 (MQ=255) cGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGc < 2:364312/147‑1 (MQ=255) cGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCgg > 2:19296/1‑149 (MQ=255) cGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCgg > 1:20966/1‑149 (MQ=255) gAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGt > 2:96548/1‑149 (MQ=255) aattcgctgctgagcatcgagccacgggtggtagcgaattCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTc < 6:78917‑M2/108‑1 (MQ=255) TTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCa < 6:367398‑M1/116‑1 (MQ=255) TTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCa > 5:367398‑M1/1‑116 (MQ=255) GCTACCACCCGTGGCTCG‑ATCCTCAGCAGCGAATTCAGGCCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAg < 5:104291‑M1/149‑1 (MQ=255) ccaccCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGtt < 3:103933/147‑1 (MQ=255) ccaccCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGa < 2:148555/149‑1 (MQ=255) cccGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCa < 3:212770/141‑1 (MQ=255) cccGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCa > 4:212770/1‑141 (MQ=255) gCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCggg < 2:20966/148‑1 (MQ=255) tCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAAc < 3:145370/69‑1 (MQ=255) tCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAAc > 4:145370/1‑69 (MQ=255) g‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGc > 3:267187/1‑149 (MQ=255) cTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCGCCCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGccc < 1:96548/148‑1 (MQ=255) tCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCAt > 3:306364/1‑148 (MQ=255) tCAGCAGCGAATTCAGGGCGGCTACCTGTTCGACTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTg < 1:175333/73‑1 (MQ=255) tCAGCAGCGAATTCAGGGCGGCTACCTGTTCGACTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTg > 2:175333/1‑73 (MQ=255) gcagcGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGAtt < 3:320986/149‑1 (MQ=255) cagcGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGAttt < 2:318376/149‑1 (MQ=255) cagcGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGAtt > 8:284990/1‑148 (MQ=255) cgaattCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTcaa > 1:353260‑M2/7‑149 (MQ=255) cgaattCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTcaa < 7:284990‑M2/143‑1 (MQ=255) aGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACctg > 7:292688/1‑149 (MQ=255) cTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCa > 8:336851/1‑149 (MQ=255) aCCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTgg < 7:298916/144‑1 (MQ=255) aCCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTAGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTgg > 8:298916/1‑144 (MQ=255) ttCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCt > 3:115197/1‑149 (MQ=255) gCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCCCTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTgg < 7:375769/149‑1 (MQ=255) gCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTgg > 7:240143/1‑149 (MQ=255) aCATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCt < 8:292688/148‑1 (MQ=255) aCATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTg > 8:353557/1‑149 (MQ=255) aTCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTcaac < 8:255134/117‑1 (MQ=255) aTCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTcaac > 7:255134/1‑117 (MQ=255) tCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAg < 8:258560/74‑1 (MQ=255) tCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAg > 7:258560/1‑74 (MQ=255) tCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGaa < 2:102226/149‑1 (MQ=255) gCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTgg > 7:184938/1‑149 (MQ=255) aGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCAc > 7:284151/1‑45 (MQ=255) aGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCAc < 8:284151/45‑1 (MQ=255) | ATACTGGCAGGGGAAGCGTTCATCGACACCGTCACCTTCGGGCTGGACGACGCACCACGCCACTACGAAATTCGCTACCACCCGTGGCTCG‑ATGCTCAGCAGCGAATTCAGGGCGGCTACCTGTTCGCCTATGACATCACGCAGCGCGTGACCGAACAGGAACGCCTGCAGCAGGCCGAAGAAGCACTGCG‑CCAGGCGCAGAAGATGGAGGCGGTCGGCCAGTTGACCGGCGGGATTGCCCATGATTTCAACAACCTGCTGGCCAGCATCCTTGGCGCGCTGGAACTGG > NC_002947/4017872‑4018160 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |