Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,050,119 C→T 7.2% T51I (ACC→ATC)  oprB‑III → carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,050,1190CT 7.2% 165.4 / 2.2 55T51I (ACC→ATC) oprB‑IIIcarbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base C (27/24);  new base T (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACAACGGCGGCAAG  >  NC_002947/4049989‑4050252
                                                                                                                                  |                                                                                                                                     
aCCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTg                                                                                                                      >  5:180419/1‑148 (MQ=255)
aCCCCCACCCTGCTCCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGa                                                                                                                     <  3:254001/149‑1 (MQ=255)
  ccccACCCTGCTGCTGGCCCTGGCCAGCACCACTGTCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGaaa                                                                                                                   >  3:173393/1‑149 (MQ=255)
  ccccACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGaaa                                                                                                                   >  1:20490/1‑149 (MQ=255)
    ccACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGCCTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGAGCCTACAGCGGTGACAcc                                                                                                                 >  5:157016/1‑149 (MQ=255)
    ccACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAAcc                                                                                                                 <  3:164049/149‑1 (MQ=255)
      aCCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGATGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGc                                                                                                               <  1:292407/149‑1 (MQ=255)
      aCCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGc                                                                                                               <  4:237897/149‑1 (MQ=255)
                tggccctggccAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTTGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCATCGGCGACTACAGc                                                                                                                          <  1:112949/128‑1 (MQ=255)
                tggccctggccAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCATCGGCGACTACAGc                                                                                                                          <  1:117828/128‑1 (MQ=255)
                tggccctggccAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCATCGGCGACTACAGc                                                                                                                          >  2:112949/1‑128 (MQ=255)
                tggccctggccAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCATCGGCGACTACAGc                                                                                                                          >  2:117828/1‑128 (MQ=255)
                    cctggccAGCACCACTGTCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACg                                                                                                 <  4:173393/149‑1 (MQ=255)
                       ggccAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTg                                                                                              <  8:258904/149‑1 (MQ=255)
                          cAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGcc                                                                                           <  3:66470/149‑1 (MQ=255)
                              accacTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCa                                                                                       <  5:321280/149‑1 (MQ=255)
                              accacTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGAGGCCTGCa                                                                                       >  4:377365/1‑149 (MQ=255)
                                      ccTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCgg                                                                               >  8:109184/1‑149 (MQ=255)
                                          gCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGgcgc                                                                            <  6:180419/148‑1 (MQ=255)
                                                 acgaTCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGgcgcgc                                                                          >  2:230208/1‑143 (MQ=255)
                                                 acgaTCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGgcgcgc                                                                          <  1:230208/143‑1 (MQ=255)
                                                      ctcATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGaa                                                               >  2:99158/1‑149 (MQ=255)
                                                        cATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCACTATTCGCAGAACg                                                             >  1:347143/1‑149 (MQ=255)
                                                             cccGCAGCACCCTGCCCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGACGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCCTTCGGCGCGCTATTCGCAGAACGTCAAg                                                        >  1:317810/1‑149 (MQ=255)
                                                             cccGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGTCTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAg                                                        >  7:191556/1‑149 (MQ=255)
                                                                gcagcaCCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTg                                                                                              >  5:364534/1‑108 (MQ=255)
                                                                gcagcaCCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTg                                                                                              <  6:364534/108‑1 (MQ=255)
                                                                gcagcaCCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTg                                                     >  2:33349/1‑149 (MQ=255)
                                                                 cagcaCCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTgg                                                    <  1:99158/149‑1 (MQ=255)
                                                                         tGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAg                                            >  3:374240/1‑149 (MQ=255)
                                                                             cGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTc                                         >  3:302581/1‑148 (MQ=255)
                                                                                 gACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCt                                    >  5:83356/1‑149 (MQ=255)
                                                                                    tGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGt                                  >  6:334668/1‑148 (MQ=255)
                                                                                      gggCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCTCCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTgg                                                    >  8:351893/1‑128 (MQ=255)
                                                                                      gggCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCTCCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTgg                                                    <  7:351893/128‑1 (MQ=255)
                                                                                      gggCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGt                                  <  5:334668/146‑1 (MQ=255)
                                                                                      gggCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTc                                 <  4:302581/147‑1 (MQ=255)
                                                                                          ggcCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACt                           <  3:377365/149‑1 (MQ=255)
                                                                                                   cATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACg                      >  4:367886/1‑145 (MQ=255)
                                                                                                   cATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACg                      <  3:367886/145‑1 (MQ=255)
                                                                                                    aTCAACTCGAAGAAGATGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTg                 >  3:168595/1‑149 (MQ=255)
                                                                                                    aTCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTg                 <  2:20490/149‑1 (MQ=255)
                                                                                                          tCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACaa            >  5:390602/1‑148 (MQ=255)
                                                                                                           cGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACAAcg          >  2:293600/1‑149 (MQ=255)
                                                                                                            gaagaaGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCt                  <  3:23507/140‑1 (MQ=255)
                                                                                                            gaagaaGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCt                  >  4:23507/1‑140 (MQ=255)
                                                                                                            gaagaaGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACaa            <  6:390602/146‑1 (MQ=255)
                                                                                                            gaagaaGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACAAcgg         >  8:213097/1‑149 (MQ=255)
                                                                                                                aagaCGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACAACggcggc     <  7:109184/149‑1 (MQ=255)
                                                                                                                  gaCGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGgcgcgc                                                                          >  7:45105/1‑78 (MQ=255)
                                                                                                                  gaCGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGgcgcgc                                                                          <  8:45105/78‑1 (MQ=255)
                                                                                                                   aCGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACAACGGCGGCAAg  >  3:222724/1‑149 (MQ=255)
                                                                                                                   aCGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACAACGGCGGCAAg  <  8:191556/149‑1 (MQ=255)
                                                                                                                   aCGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACAACGGCGGCAAg  >  3:139768/1‑149 (MQ=255)
                                                                                                                   aCGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACAACGGCGGCAAg  <  4:222724/149‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
ACCCCCACCCTGCTGCTGGCCCTGGCCAGCACCACTGCCCTTGCCAGCGACGATCTCATGACCCGCAGCACCCTGACCGGTGACTGGGGCGGCCTGCGCCATCAACTCGAAGAAGACGGCGTCAAGATCACCGGCGACTACAGCGGTGAAACCGCCTACAACGCCCACGGTGGCCTGCACCGTTCGGCGCGCTATTCGCAGAACGTCAAGCTGGGCGTGCAGTTCGACCTGTCGAAACTGTACGGCCTGGACAACGGCGGCAAG  >  NC_002947/4049989‑4050252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑