Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,065,982	C→G	10.5%	D1028H (GAC→CAC)	mdtC ←	multidrug transporter membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,065,982	0	C	G	10.5%	165.3 / 5.9	55	D1028H (GAC→CAC)	mdtC	multidrug transporter membrane protein
Reads supporting (aligned to +/- strand):  ref base C (23/26);  new base G (3/3);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGTCGGGGCTCAGGGTACAGGCACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGC  >  NC_002947/4065834‑4066128                                                                                                                                                     |                                                                                                                                                     gtAGTCGGGGCTCAGTGTACAGGCACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTc                                                                                                                                                      >  6:133426/1‑149 (MQ=255)        gggCTCAGGGTACAGGCACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTacg                                                                                                                                                >  8:170734/1‑148 (MQ=255)            tCAGGGTACAGGCACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTGCGTaccgacgc                                                                                                                                           >  7:193875/1‑149 (MQ=255)                  tACAGGCACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTGCGTACCGAcgcc‑gctct                                                                                                                                    <  8:193875/149‑4 (MQ=255)                     aGGCACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAAc                                                                                                                                 >  2:381397/1‑149 (MQ=255)                     aGGCACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGAGGCGTCGGTACGCACGCC‑TCGCCAAc                                                                                                                                 >  6:257830/1‑149 (MQ=255)                       gCACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACgg                                                                                                                               >  3:337246/1‑149 (MQ=255)                         aCTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACgg                                                                                                                               <  6:199379/147‑1 (MQ=255)                         aCTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACgg                                                                                                                               >  5:199379/1‑147 (MQ=255)                         aCTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGtt                                                                                                                             <  5:133426/149‑1 (MQ=255)                              gCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAAc                                                                                                                        <  7:170734/149‑1 (MQ=255)                                 aGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGt                                                                                                                     >  8:255805/1‑149 (MQ=255)                                  ggCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTg                                                                                                                    >  1:70031/1‑149 (MQ=255)                                            aaCCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTGCGTACCGAcgcc‑gct                                                                                                                                      <  3:103436/121‑2 (MQ=255)                                            aaCCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTGCGTACCGAcgcc‑gct                                                                                                                                      >  4:103436/1‑120 (MQ=255)                                            aaCCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGGCATAGCGGGGTTTCCAGAGCGGCGGCGGTACGCACGCCTGCGCCACCGTTTCAACCGTTGgcgcggcgt                                                                                                           <  7:161852/149‑6 (MQ=38)                                              cccGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTc                                                                                                        <  2:70031/149‑1 (MQ=255)                                                 ggCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcag                                                                                                     <  4:337246/149‑1 (MQ=255)                                                 ggCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcag                                                                                                     >  1:42516/1‑149 (MQ=255)                                                  gCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcagg                                                                                                    <  3:156037/149‑1 (MQ=255)                                                     ccGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGGCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggtac                                                                                                 <  6:417330/149‑1 (MQ=255)                                                      cGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggtaca                                                                                                <  1:381397/149‑1 (MQ=255)                                                       gAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggtacag                                                                                               <  5:200794/149‑1 (MQ=255)                                                       gAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggtacag                                                                                               <  5:179648/149‑1 (MQ=255)                                                        aGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggtacagg                                                                                              >  2:292239/1‑149 (MQ=255)                                                               gcgAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGtt                                                                                                                             <  6:342199/111‑1 (MQ=255)                                                               gcgAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGtt                                                                                                                             >  5:342199/1‑111 (MQ=255)                                                               gcgAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAacca                                                                                       >  5:315077/1‑149 (MQ=255)                                                                  aGCCACGCCCACGGTTCAGTGGTTGCCGGGCACGGTGAAGTGGGGTCTGGCCAAAAGTCATACCGGGGTTTCCAGAGCGGCGTCGGTACGAACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCg                                                                                    <  5:257830/149‑1 (MQ=255)                                                                     cacggccacggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcg                                                                                 >  3:250576/1‑149 (MQ=255)                                                                      acggccacggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcgt                                                                                >  7:197030/1‑149 (MQ=255)                                                                          ccacggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGta                                                                            >  3:23392/1‑149 (MQ=255)                                                                                    gTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGa                                                                  <  4:250576/149‑1 (MQ=255)                                                                                          gCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcgtc                                                                               <  3:267834/130‑1 (MQ=255)                                                                                          gCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcgtc                                                                               >  4:267834/1‑130 (MQ=255)                                                                                            aGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTgg                                                          <  7:255805/149‑1 (MQ=255)                                                                                              ggCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCt                                                        <  7:151760/149‑1 (MQ=255)                                                                                                 aCGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGaa                                                     <  5:114925/149‑1 (MQ=255)                                                                                                   ggTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAaca                                                   >  3:205669/1‑149 (MQ=255)                                                                                                       aaGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAg                                              <  4:23392/149‑1 (MQ=255)                                                                                                              gTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCAcc                                        >  5:369450/1‑148 (MQ=255)                                                                                                              gTCTGGGCAAAAGTCATAGAGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCAcc                                        >  4:387345/1‑148 (MQ=255)                                                                                                                    gCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCgatg                                    <  4:298699/146‑1 (MQ=255)                                                                                                                    gCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCgatg                                    >  3:298699/1‑146 (MQ=255)                                                                                                                    gCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGACGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGatgatg                                 >  1:44794/1‑149 (MQ=255)                                                                                                                      aaaaGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGt                               >  6:54190/1‑149 (MQ=255)                                                                                                                        aaGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGt                               >  4:385437/1‑147 (MQ=255)                                                                                                                          gTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGTCAgg                           <  5:54190/149‑1 (MQ=255)                                                                                                                          gTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGTCAgg                           <  4:205669/149‑1 (MQ=255)                                                                                                                                gCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGTCAGGCCCAg                      <  6:369450/148‑1 (MQ=255)                                                                                                                                gCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGTCAGGCCCAGc                     >  1:155360/1‑149 (MQ=255)                                                                                                                                           ggcgcggcgTGCGTACCGACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGta                                                                            >  1:152032/4‑84 (MQ=38)                                                                                                                                           ggcgcggcgTGCGTACCGACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGta                                                                            <  2:152032/81‑1 (MQ=38)                                                                                                                                               cggcgTCGGTACGCACGGC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCt      <  8:197030/149‑1 (MQ=255)                                                                                                                                                  cgTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTgc    <  1:292239/149‑1 (MQ=255)                                                                                                                                                   gTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTgcgc  <  3:387345/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GTAGTCGGGGCTCAGGGTACAGGCACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCC‑GCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACA‑CCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGC  >  NC_002947/4065834‑4066128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑