Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,114,329 G→T 8.7% intergenic (+76/+70) PP_3619 → / ← ycaO hypothetical protein/ribosomal protein S12 methylthiotransferase accessory factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,114,3290GT 8.7% 140.9 / 4.3 46intergenic (+76/+70)PP_3619/ycaOhypothetical protein/ribosomal protein S12 methylthiotransferase accessory factor
Reads supporting (aligned to +/- strand):  ref base G (24/18);  new base T (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

AAGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTC  >  NC_002947/4114182‑4114473
                                                                                                                                                   |                                                                                                                                                   
aaGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCgg                                                                                                                                                    >  4:389967/1‑149 (MQ=255)
      ttCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGAAAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAg                                                                                                                                               <  7:75020/148‑1 (MQ=255)
         aaTGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGAtct                                                                                                                                           >  4:87199/1‑149 (MQ=255)
          aTGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGAtctc                                                                                                                                          <  3:389967/149‑1 (MQ=255)
             tCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGAtctc                                                                                                                                          >  5:18356/1‑146 (MQ=255)
               cGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAagag                                                                                                                                     <  6:18356/149‑1 (MQ=255)
                    aGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTg                                                                                                                                <  8:172056/149‑1 (MQ=255)
                          accaccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATAc                                                                                                                          <  8:170462/149‑1 (MQ=255)
                           ccaccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCCTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATAcc                                                                                                                         >  5:13094/1‑149 (MQ=255)
                            caccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCt                                                                                                                        <  3:87199/149‑1 (MQ=255)
                                 gcggAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTggac                                                                                                                  >  7:311347/1‑147 (MQ=21)
                                    gAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTggacgct                                                                                                               <  8:311347/149‑6 (MQ=18)
                                     aGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTg                                                                                                              <  6:310605/149‑1 (MQ=255)
                                            cGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGcc                                                                                                       >  1:98521/1‑149 (MQ=255)
                                                          cTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                         >  6:378922/1‑149 (MQ=255)
                                                          cTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                         >  2:238417/1‑149 (MQ=255)
                                                           tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCaa                                                                                                                                        >  7:357772/1‑102 (MQ=255)
                                                           tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCaa                                                                                                                                        <  8:357772/102‑1 (MQ=255)
                                                           tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgcg                                                                                        >  1:277566/1‑149 (MQ=255)
                                                                  gAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGcc                                                                                 >  8:207496/1‑149 (MQ=255)
                                                                        tCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACTGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAg                                                                           >  1:73704/1‑149 (MQ=255)
                                                                        tCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAg                                                                           >  2:263020/1‑149 (MQ=255)
                                                                             agcTGATGTGTTTAGGATGATACCGCTATGGGCGTGGATGCCAACGCGCCACGCCAGGGTCCAGTGTTGCGCCACAGTTCTCAACAGCGGTGAAAAACGTGTGG‑CGGTGGGTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGaa                                                                      <  8:244043/147‑1 (MQ=255)
                                                                             accTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCCGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTCCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGaa                                                                      <  5:207919/149‑1 (MQ=255)
                                                                              ccTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAAc                                                                     <  5:378922/149‑1 (MQ=255)
                                                                               cTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACt                                                                    >  5:192215/1‑149 (MQ=255)
                                                                               cTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGAGATGATTGGGCGCAGCGCCTGTCAGGCGAACt                                                                    >  6:290323/1‑149 (MQ=255)
                                                                                                       tATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                         >  3:396666/1‑104 (MQ=255)
                                                                                                         tGCGCCTGGAGGGCAACGCGGCAAGCCAGCGTCCACAGTTTCGCCACATATCTCAAGAGCGTTGCAATACCTGGGG‑CGCGGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGcttct‑t                                         <  2:98521/148‑1 (MQ=255)
                                                                                                            gcCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAg                                     <  6:192215/149‑1 (MQ=255)
                                                                                                             ccTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCCTG‑CAGCTTAT‑Tg                                        >  3:223043/1‑145 (MQ=255)
                                                                                                                ggATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGt                                 >  7:220751/1‑149 (MQ=255)
                                                                                                                      cAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGAt                             >  5:364591/1‑147 (MQ=255)
                                                                                                                       aaCGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCt                                                                                >  5:161823/1‑97 (MQ=255)
                                                                                                                        aCGCGGCAACCCAGCGTCCACACGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTCCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAgg                         >  1:180693/1‑149 (MQ=255)
                                                                                                                          gcgGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGgcg                       >  5:233105/1‑149 (MQ=255)
                                                                                                                            ggCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGgcg                       >  7:52477/1‑147 (MQ=255)
                                                                                                                             gCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTGCCAGCTTCT‑TGTAGCTGTCGATCAGGCGCa                     <  1:238417/149‑1 (MQ=255)
                                                                                                                               aaGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTGGACGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGt                                 <  4:358230/134‑1 (MQ=14)
                                                                                                                               aaGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTGGACGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGt                                 >  3:358230/1‑134 (MQ=14)
                                                                                                                                  ccAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGc               >  8:46713/1‑149 (MQ=255)
                                                                                                                                      cGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGAt            >  4:119993/1‑148 (MQ=255)
                                                                                                                                        tCCACAGGTTCGGCACAGATATCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGTCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCaa         <  3:317193/149‑1 (MQ=255)
                                                                                                                                         ccACAGGTTCGGCACCGAGCTCACGCGGGGTTCAATACCTGGGG‑CGGGGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAg        <  8:220751/149‑1 (MQ=255)
                                                                                                                                          cacaTGTTCGGCACATATCCAAATTGCGGTGCAATACCTGTGG‑GGGTGGGTTGCCGGCGATGTTTGGGCGCATCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTATGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAg        <  1:263020/149‑1 (MQ=255)
                                                                                                                                            caGGTTCGGCACAGATCTCAAGAGCGGTGCATTACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAg                                                <  4:378421/107‑1 (MQ=255)
                                                                                                                                               gTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTc  >  8:190946/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTC  >  NC_002947/4114182‑4114473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑