Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,114,336 A→C 7.9% intergenic (+83/+63) PP_3619 → / ← ycaO hypothetical protein/ribosomal protein S12 methylthiotransferase accessory factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,114,3360AC 7.9% 139.0 / 3.2 50intergenic (+83/+63)PP_3619/ycaOhypothetical protein/ribosomal protein S12 methylthiotransferase accessory factor
Reads supporting (aligned to +/- strand):  ref base A (27/18);  new base C (2/2);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

AATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTCGAGCTTCAT  >  NC_002947/4114191‑4114482
                                                                                                                                                 |                                                                                                                                                     
aaTGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGAtct                                                                                                                                                    >  4:87199/1‑149 (MQ=255)
 aTGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGAtctc                                                                                                                                                   <  3:389967/149‑1 (MQ=255)
    tCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGAtctc                                                                                                                                                   >  5:18356/1‑146 (MQ=255)
      cGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAagag                                                                                                                                              <  6:18356/149‑1 (MQ=255)
           aGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTg                                                                                                                                         <  8:172056/149‑1 (MQ=255)
                 accaccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATAc                                                                                                                                   <  8:170462/149‑1 (MQ=255)
                  ccaccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCCTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATAcc                                                                                                                                  >  5:13094/1‑149 (MQ=255)
                   caccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCt                                                                                                                                 <  3:87199/149‑1 (MQ=255)
                        gcggAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTggac                                                                                                                           >  7:311347/1‑147 (MQ=21)
                           gAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTggacgct                                                                                                                        <  8:311347/149‑6 (MQ=18)
                            aGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTg                                                                                                                       <  6:310605/149‑1 (MQ=255)
                                   cGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGcc                                                                                                                >  1:98521/1‑149 (MQ=255)
                                                 cTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                                  >  2:238417/1‑149 (MQ=255)
                                                 cTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                                  >  6:378922/1‑149 (MQ=255)
                                                  tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCaa                                                                                                                                                 >  7:357772/1‑102 (MQ=255)
                                                  tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCaa                                                                                                                                                 <  8:357772/102‑1 (MQ=255)
                                                  tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgcg                                                                                                 >  1:277566/1‑149 (MQ=255)
                                                         gAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGcc                                                                                          >  8:207496/1‑149 (MQ=255)
                                                               tCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACTGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAg                                                                                    >  1:73704/1‑149 (MQ=255)
                                                               tCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAg                                                                                    >  2:263020/1‑149 (MQ=255)
                                                                    agcTGATGTGTTTAGGATGATACCGCTATGGGCGTGGATGCCAACGCGCCACGCCAGGGTCCAGTGTTGCGCCACAGTTCTCAACAGCGGTGAAAAACGTGTGG‑CGGTGGGTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGaa                                                                               <  8:244043/147‑1 (MQ=255)
                                                                    accTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCCGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTCCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGaa                                                                               <  5:207919/149‑1 (MQ=255)
                                                                     ccTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAAc                                                                              <  5:378922/149‑1 (MQ=255)
                                                                      cTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACt                                                                             >  5:192215/1‑149 (MQ=255)
                                                                      cTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGAGATGATTGGGCGCAGCGCCTGTCAGGCGAACt                                                                             >  6:290323/1‑149 (MQ=255)
                                                                                              tATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                                  >  3:396666/1‑104 (MQ=255)
                                                                                                tGCGCCTGGAGGGCAACGCGGCAAGCCAGCGTCCACAGTTTCGCCACATATCTCAAGAGCGTTGCAATACCTGGGG‑CGCGGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGcttct‑t                                                  <  2:98521/148‑1 (MQ=255)
                                                                                                   gcCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAg                                              <  6:192215/149‑1 (MQ=255)
                                                                                                    ccTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCCTG‑CAGCTTAT‑Tg                                                 >  3:223043/1‑145 (MQ=255)
                                                                                                       ggATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGt                                          >  7:220751/1‑149 (MQ=255)
                                                                                                             cAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGAt                                      >  5:364591/1‑147 (MQ=255)
                                                                                                              aaCGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCt                                                                                         >  5:161823/1‑97 (MQ=255)
                                                                                                               aCGCGGCAACCCAGCGTCCACACGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTCCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAgg                                  >  1:180693/1‑149 (MQ=255)
                                                                                                                 gcgGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGgcg                                >  5:233105/1‑149 (MQ=255)
                                                                                                                   ggCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGgcg                                >  7:52477/1‑147 (MQ=255)
                                                                                                                    gCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTGCCAGCTTCT‑TGTAGCTGTCGATCAGGCGCa                              <  1:238417/149‑1 (MQ=255)
                                                                                                                      aaGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTGGACGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGt                                          <  4:358230/134‑1 (MQ=14)
                                                                                                                      aaGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTGGACGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGt                                          >  3:358230/1‑134 (MQ=14)
                                                                                                                         ccAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGc                        >  8:46713/1‑149 (MQ=255)
                                                                                                                             cGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGAt                     >  4:119993/1‑148 (MQ=255)
                                                                                                                               tCCACAGGTTCGGCACAGATATCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGTCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCaa                  <  3:317193/149‑1 (MQ=255)
                                                                                                                                ccACAGGTTCGGCACCGAGCTCACGCGGGGTTCAATACCTGGGG‑CGGGGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAg                 <  8:220751/149‑1 (MQ=255)
                                                                                                                                 cacaTGTTCGGCACATATCCAAATTGCGGTGCAATACCTGTGG‑GGGTGGGTTGCCGGCGATGTTTGGGCGCATCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTATGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAg                 <  1:263020/149‑1 (MQ=255)
                                                                                                                                   caGGTTCGGCACAGATCTCAAGAGCGGTGCATTACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAg                                                         <  4:378421/107‑1 (MQ=255)
                                                                                                                                      gTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTc           >  8:190946/1‑149 (MQ=255)
                                                                                                                                           gCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑Tg                                                 >  6:68790/1‑106 (MQ=255)
                                                                                                                                           gCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑Tg                                                 <  5:68790/106‑1 (MQ=255)
                                                                                                                                           gCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCaa                  >  6:560/1‑137 (MQ=255)
                                                                                                                                           gCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCaa                  <  5:560/137‑1 (MQ=255)
                                                                                                                                               aGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTCGAGCTTCAt  >  3:250810/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
AATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTCGAGCTTCAT  >  NC_002947/4114191‑4114482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑