Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,114,345 G→T 8.3% intergenic (+92/+54) PP_3619 → / ← ycaO hypothetical protein/ribosomal protein S12 methylthiotransferase accessory factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,114,3450GT 8.3% 141.8 / 3.7 48intergenic (+92/+54)PP_3619/ycaOhypothetical protein/ribosomal protein S12 methylthiotransferase accessory factor
Reads supporting (aligned to +/- strand):  ref base G (27/17);  new base T (2/2);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

CGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTCGAGCTTCATGCTGGTC  >  NC_002947/4114197‑4114489
                                                                                                                                                    |                                                                                                                                                   
cGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAagag                                                                                                                                                     <  6:18356/149‑1 (MQ=255)
     aGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTg                                                                                                                                                <  8:172056/149‑1 (MQ=255)
           accaccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATAc                                                                                                                                          <  8:170462/149‑1 (MQ=255)
            ccaccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCCTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATAcc                                                                                                                                         >  5:13094/1‑149 (MQ=255)
             caccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCt                                                                                                                                        <  3:87199/149‑1 (MQ=255)
                  gcggAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTggac                                                                                                                                  >  7:311347/1‑147 (MQ=21)
                     gAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTggacgct                                                                                                                               <  8:311347/149‑6 (MQ=18)
                      aGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTg                                                                                                                              <  6:310605/149‑1 (MQ=255)
                             cGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGcc                                                                                                                       >  1:98521/1‑149 (MQ=255)
                                           cTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                                         >  2:238417/1‑149 (MQ=255)
                                           cTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                                         >  6:378922/1‑149 (MQ=255)
                                            tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgcg                                                                                                        >  1:277566/1‑149 (MQ=255)
                                                   gAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGcc                                                                                                 >  8:207496/1‑149 (MQ=255)
                                                         tCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACTGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAg                                                                                           >  1:73704/1‑149 (MQ=255)
                                                         tCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAg                                                                                           >  2:263020/1‑149 (MQ=255)
                                                              agcTGATGTGTTTAGGATGATACCGCTATGGGCGTGGATGCCAACGCGCCACGCCAGGGTCCAGTGTTGCGCCACAGTTCTCAACAGCGGTGAAAAACGTGTGG‑CGGTGGGTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGaa                                                                                      <  8:244043/147‑1 (MQ=255)
                                                              accTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCCGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTCCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGaa                                                                                      <  5:207919/149‑1 (MQ=255)
                                                               ccTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAAc                                                                                     <  5:378922/149‑1 (MQ=255)
                                                                cTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACt                                                                                    >  5:192215/1‑149 (MQ=255)
                                                                cTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGAGATGATTGGGCGCAGCGCCTGTCAGGCGAACt                                                                                    >  6:290323/1‑149 (MQ=255)
                                                                                        tATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                                         >  3:396666/1‑104 (MQ=255)
                                                                                          tGCGCCTGGAGGGCAACGCGGCAAGCCAGCGTCCACAGTTTCGCCACATATCTCAAGAGCGTTGCAATACCTGGGG‑CGCGGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGcttct‑t                                                         <  2:98521/148‑1 (MQ=255)
                                                                                             gcCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAg                                                     <  6:192215/149‑1 (MQ=255)
                                                                                              ccTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCCTG‑CAGCTTAT‑Tg                                                        >  3:223043/1‑145 (MQ=255)
                                                                                                 ggATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGt                                                 >  7:220751/1‑149 (MQ=255)
                                                                                                       cAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGAt                                             >  5:364591/1‑147 (MQ=255)
                                                                                                        aaCGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCt                                                                                                >  5:161823/1‑97 (MQ=255)
                                                                                                         aCGCGGCAACCCAGCGTCCACACGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTCCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAgg                                         >  1:180693/1‑149 (MQ=255)
                                                                                                           gcgGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGgcg                                       >  5:233105/1‑149 (MQ=255)
                                                                                                             ggCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGgcg                                       >  7:52477/1‑147 (MQ=255)
                                                                                                              gCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTGCCAGCTTCT‑TGTAGCTGTCGATCAGGCGCa                                     <  1:238417/149‑1 (MQ=255)
                                                                                                                aaGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTGGACGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGt                                                 <  4:358230/134‑1 (MQ=14)
                                                                                                                aaGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTGGACGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGt                                                 >  3:358230/1‑134 (MQ=14)
                                                                                                                   ccAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGc                               >  8:46713/1‑149 (MQ=255)
                                                                                                                       cGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGAt                            >  4:119993/1‑148 (MQ=255)
                                                                                                                         tCCACAGGTTCGGCACAGATATCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGTCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCaa                         <  3:317193/149‑1 (MQ=255)
                                                                                                                          ccACAGGTTCGGCACCGAGCTCACGCGGGGTTCAATACCTGGGG‑CGGGGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAg                        <  8:220751/149‑1 (MQ=255)
                                                                                                                           cacaTGTTCGGCACATATCCAAATTGCGGTGCAATACCTGTGG‑GGGTGGGTTGCCGGCGATGTTTGGGCGCATCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTATGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAg                        <  1:263020/149‑1 (MQ=255)
                                                                                                                             caGGTTCGGCACAGATCTCAAGAGCGGTGCATTACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAg                                                                <  4:378421/107‑1 (MQ=255)
                                                                                                                                gTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTc                  >  8:190946/1‑149 (MQ=255)
                                                                                                                                     gCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑Tg                                                        <  5:68790/106‑1 (MQ=255)
                                                                                                                                     gCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑Tg                                                        >  6:68790/1‑106 (MQ=255)
                                                                                                                                     gCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCaa                         >  6:560/1‑137 (MQ=255)
                                                                                                                                     gCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCaa                         <  5:560/137‑1 (MQ=255)
                                                                                                                                         aGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTCGAGCTTCAt         >  3:250810/1‑149 (MQ=255)
                                                                                                                                              tcAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTCGAGCTTCATGCTgg    >  5:40461/1‑149 (MQ=255)
                                                                                                                                                aaGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCg                                                                                        >  4:58202/1‑65 (MQ=255)
                                                                                                                                                aaGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTCGAGCTTCATGCTGGTc  <  7:190946/149‑1 (MQ=255)
                                                                                                                                                aaGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTCGAGCTTCATGCTGGTc  >  1:18176/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCT‑TGTAGCTGTCGATCAGGCGCAGGTGGCGATCAAGGCCTTCGAGCTTCATGCTGGTC  >  NC_002947/4114197‑4114489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑