Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 445,087 G→A 7.3% intergenic (+60/‑228) PP_0367 → / → PP_0368 hypothetical protein/acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947445,0870GA 7.3% 155.2 / 2.1 55intergenic (+60/‑228)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base A (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

TGGGCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGA  >  NC_002947/444942‑445229
                                                                                                                                                 |                                                                                                                                              
tGGGCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGt                                                                                                                                             <  1:379559/149‑1 (MQ=255)
    cAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGa                                                                                                                                         <  1:109375/149‑1 (MQ=255)
    cAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGa                                                                                                                                         >  6:194308/1‑149 (MQ=255)
       gACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTCTAGGAGAGGGCTTGACCGGGAATGCGTCCGaag                                                                                                                                       >  5:118820/1‑146 (MQ=255)
               tCGATCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGAcaacaa                                                                                                                              <  8:98612/149‑1 (MQ=255)
               tCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGa                                                                                                                                         >  8:360811/1‑138 (MQ=255)
               tCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGa                                                                                                                                         <  7:360811/138‑1 (MQ=255)
                   gCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAAtttt                                                                                                                          >  4:29504/1‑149 (MQ=255)
                       ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGt                                                                                                                        <  2:144513/147‑1 (MQ=255)
                       ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt                                                                                                                      <  3:29504/149‑1 (MQ=255)
                       ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCCCGAATTCGTCCGATCAGACAAAAATTTTGtgt                                                                                                                      >  7:27770/1‑147 (MQ=255)
                               ggTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGc                                                                                                              <  5:194308/149‑1 (MQ=255)
                                 tcgtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATc                                                                                                                                       <  7:32243/122‑1 (MQ=255)
                                 tcgtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATc                                                                                                                                       >  8:32243/1‑122 (MQ=255)
                                   gtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGAt                                                                                                                                        <  7:148340/119‑1 (MQ=255)
                                   gtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGAt                                                                                                                                        >  8:148340/1‑119 (MQ=255)
                                         gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg                                                                                                    <  6:83789/149‑1 (MQ=18)
                                              cgacgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGTATGCGTCCGATCAGACAACAATTTTGTCTGGt                                                                                                                   <  7:25324/129‑1 (MQ=255)
                                              cgacgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGTATGCGTCCGATCAGACAACAATTTTGTCTGGt                                                                                                                   >  8:25324/1‑129 (MQ=255)
                                                acgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGGATTCCCGCGAATGCGGCCGAAGAGCCAAAAAATTTTTATGGTGGGGGCTCGTAGCGGGGCAGcc                                                                                             >  5:226943/1‑149 (MQ=255)
                                                        cTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg                                                                                                    <  1:283880/134‑1 (MQ=17)
                                                        cTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg                                                                                                    >  2:283880/1‑134 (MQ=17)
                                                                gATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGCATGCGTCCGATCAGACAACAATTTTGTGTGGTCGGGCCTCTTCGCGGGCAAGCCCGCGCCTAAAAggggt                                                                             >  3:322540/1‑147 (MQ=255)
                                                                  tcatcGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGAATGCGTCCGATAAGACAACAATTTTTTCTGGTCGGGCCTCTTAGCGGGCAATCCCGCTCCGACAACGGTTtg                                                                           >  6:101027/1‑149 (MQ=255)
                                                                        acacCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGTTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGtggcat                                                                     >  5:88123/1‑149 (MQ=255)
                                                                        acacCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt                                                                                                                      <  2:342555/100‑1 (MQ=255)
                                                                                   tGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAAtttt                                                                                                                          >  2:78828/1‑85 (MQ=255)
                                                                                      aTACTTGCGTCATCCCTTATTTCTGCGCCTGCCTTGTTGGTGGGGGCTTGCCGGCTATTGCGTCCTATCACAAAAAATTTTTGTCTGGTCGGGCCTCTTCGCGGGAAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAttt                                                       <  2:341892/149‑1 (MQ=255)
                                                                                          ttGCGTCATCCACTATTTCAGCGCCTGCCTTGTAGGAGCGGGCATGCCCGCGAATGAGTCCCATAACACAACAAAATTTTCTGGTGGGGCCTCGTACCGGGCAAGCCAGGGCCTACAAGCGTTTGAGGCATTGCATGCCAGCTTTggc                                                    >  2:263719/1‑146 (MQ=255)
                                                                                                 aTCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGTTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCg                                            <  6:88123/149‑1 (MQ=255)
                                                                                                   ccccTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGAATTCGTCCCATAAGACAACAATTTTTTATGGTCGGGCCACTTAGCGGGCCAGCCCCCTCCGAAAAGGGTTTTTGGCATGGCAAGTCTGATTTTGCATTTTTAGTg                                          >  2:32186/1‑149 (MQ=255)
                                                                                                           tCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTcg                                                                                                       >  4:278652/1‑80 (MQ=255)
                                                                                                           tCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTcg                                                                                                       <  3:278652/80‑1 (MQ=255)
                                                                                                                ccctgcctTGTTGCTTCGGGCTTGCCCGCGAATGCGTCCGATCATCAAACAATTTTGGCTGGTCGGGACTCTTCGCGGGCATGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCa                              <  1:263719/147‑1 (MQ=255)
                                                                                                                              cgCGGGCTGCCCCGCTAAGGCGTCCGATCCCACAAAAATTTTGTCTGGCGGGGCCTCTCCGCGGGAAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCtt               <  6:226943/148‑1 (MQ=255)
                                                                                                                                cGGGTTTGCCCGCTAATGCGGCCGATCACAAAACAATTTTGTCGGGGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa             <  8:27770/149‑1 (MQ=255)
                                                                                                                                       gCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGt                       >  6:347114/1‑132 (MQ=255)
                                                                                                                                       gCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGt                       <  5:347114/132‑1 (MQ=255)
                                                                                                                                        cccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCTTAAATGTCACCATAGTCCATCCTTGACAGAAAGc     >  2:174557/1‑149 (MQ=255)
                                                                                                                                        cccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAg      <  1:47634/148‑1 (MQ=255)
                                                                                                                                        cccGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc     >  5:39090/1‑149 (MQ=18)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCt                >  6:327631/1‑137 (MQ=255)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCt                <  5:327631/137‑1 (MQ=255)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa             >  8:350400/1‑140 (MQ=255)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa             <  7:350400/140‑1 (MQ=255)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt    >  1:229380/1‑149 (MQ=255)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt    >  5:275895/1‑149 (MQ=255)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt    >  5:134588/1‑149 (MQ=255)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt    >  4:178987/1‑149 (MQ=255)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt    >  4:21296/1‑149 (MQ=255)
                                                                                                                                         ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTACTACAAGGGTTTGTGGCATGGCATGACTGATTTGCAATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAgaa    >  4:230645/1‑147 (MQ=255)
                                                                                                                                           gctaaTGCGTCCGATCAGACAACAATTTTGTCTGGGCGGGCCTATTCGCGGGAAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa  <  1:221988/146‑1 (MQ=255)
                                                                                                                                           gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCa                    >  6:423140/1‑131 (MQ=255)
                                                                                                                                           gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCa                    <  5:423140/131‑1 (MQ=255)
                                                                                                                                           gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt    >  2:106113/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TGGGCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGA  >  NC_002947/444942‑445229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑