Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,282,355	A→G	9.0%	L292P (CTG→CCG)	PP_3753 ←	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,282,355	0	A	G	9.0%	151.7 / 4.0	55	L292P (CTG→CCG)	PP_3753	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (25/25);  new base G (3/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCG  >  NC_002947/4282208‑4282500                                                                                                                                                     |                                                                                                                                                  cTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAg                                                                                                                                                  >  3:214145/1‑149 (MQ=255)  tACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGc                                                                                                                                                 >  7:34079/1‑149 (MQ=255)   aCAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGcc                                                                                                                                                >  2:14982/1‑149 (MQ=255)     aGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGc                                                                                                                                              >  7:268709/1‑149 (MQ=255)      ggTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCt                                                                                                                                             >  7:99722/1‑149 (MQ=255)          gAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCg                                                                                                                                           >  1:151382/1‑147 (MQ=255)          gAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGaa                                                                                                                                         <  1:148473/149‑1 (MQ=255)          gAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGA‑GCGGCTGcgcg                                                                                                                                           >  7:217083/1‑147 (MQ=255)             cGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGA‑GCGGCTGCGCGAAGGc                                                                                                                                      <  8:217083/149‑1 (MQ=255)               cAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGA‑GCGGCTGCGCGAAGGcgc                                                                                                                                    <  7:134392/149‑3 (MQ=255)                aGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCAt                                                                                                                                   <  2:151382/149‑1 (MQ=255)                 ggCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGc                                                                                                                                                 >  4:62953/1‑134 (MQ=255)                 ggCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGc                                                                                                                                                 <  3:62953/134‑1 (MQ=255)                    cGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGcc                                                                                                                               >  2:2484/1‑149 (MQ=255)                     gACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCAt                                                                                                                                   >  7:68185/1‑144 (MQ=255)                     gACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCAt                                                                                                                                   <  8:68185/144‑1 (MQ=255)                                   ggTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATcc                                                                                                                <  8:268709/149‑1 (MQ=255)                                   ggTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCGCGAAGGCCATGGCCCGGCGCACTTCATcc                                                                                                                >  7:369124/1‑149 (MQ=255)                                    gTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCt                                                                                                               <  2:79578/149‑1 (MQ=255)                                           ccTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATTCTCAAAGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGAGCAATTCATCCTTCATct                                                                                                         >  4:260547/1‑148 (MQ=255)                                                gCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGt                                                                                                   <  8:369124/149‑1 (MQ=255)                                                      cccTGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCTGTTCCTTAAACCCCGCCTGCTCGGCATTTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACg                                                                                                <  2:341571/146‑1 (MQ=255)                                                        cGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGc                                                                                           <  6:161991/149‑1 (MQ=255)                                                            ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATc                                                                                                                 <  2:16165/123‑1 (MQ=255)                                                            ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATc                                                                                                                 >  1:16165/1‑123 (MQ=255)                                                            ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGcc                                                                                          >  6:259772/1‑146 (MQ=255)                                                            ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGcc                                                                                          <  5:259772/146‑1 (MQ=255)                                                             cACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTc                                                                                      <  8:99722/149‑1 (MQ=255)                                                              aCTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTcc                                                                                     <  1:14982/149‑1 (MQ=255)                                                                          cccGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGc                                                                         >  7:24689/1‑149 (MQ=255)                                                                                   aGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAg                                                                >  5:84073/1‑149 (MQ=255)                                                                                   aGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAg                                                                <  4:214145/149‑1 (MQ=255)                                                                                        cTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACt                                                           >  2:71957/1‑149 (MQ=255)                                                                                             ttCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCAc                                                      <  8:24689/149‑1 (MQ=255)                                                                                                cTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGAt                                                                                                           >  1:44984/1‑93 (MQ=255)                                                                                                cTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGAt                                                                                                           <  2:44984/93‑1 (MQ=255)                                                                                                 tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCg                                                                    >  4:372417/1‑131 (MQ=255)                                                                                                 tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCg                                                                    <  3:372417/131‑1 (MQ=255)                                                                                                 tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCAc                                                      <  5:137144/145‑1 (MQ=255)                                                                                                 tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCAc                                                      >  6:137144/1‑145 (MQ=255)                                                                                                 tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGAt                                                  >  7:6080/1‑149 (MQ=255)                                                                                                  gAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGAt                                                  >  1:96076/1‑149 (MQ=255)                                                                                                    aaCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATccc                                               <  3:327149/149‑1 (MQ=255)                                                                                                     aCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTg                                                    >  5:126135/1‑143 (MQ=255)                                                                                                     aCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTg                                                    <  6:126135/143‑1 (MQ=255)                                                                                                           ccTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTtgc                                        <  1:2484/149‑1 (MQ=255)                                                                                                               cTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTtgctgc                                     <  8:34079/148‑1 (MQ=255)                                                                                                                  ggCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTgcgc                                   >  3:168693/1‑147 (MQ=255)                                                                                                                           ggCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCa                        <  1:71957/149‑1 (MQ=255)                                                                                                                                           ccTTCGA‑GCGGCTGCGCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTc         >  7:238878/1‑148 (MQ=255)                                                                                                                                            cTTCGA‑GCGGCTGCGCGAAGGCCATGGCCCGGCGCACTTCATGCTTGATCTGCTGGTACCAACGCCCCTCCCGCTCCAACTTCCGGCGAAAGGTACAGGCACTGATCCCCTGTTGCTGAGCCAGTGCCGCCAATGTCCGCCACTGTcc        >  4:373247/1‑148 (MQ=255)                                                                                                                                               cgc‑gCAGCCGCTCGCAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGTTACTGGCACTGATCCGCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATa     <  3:260547/148‑1 (MQ=255)                                                                                                                                                   gcAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCGAACTGGCGGCGAAAGGTACTGGCACTGAt                                                  <  5:339706/100‑1 (MQ=255)                                                                                                                                                   gcAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAgcg  >  4:211008/1‑148 (MQ=255)                                                                                                                                                   gcAGCCGCTCGAAGGCCATCGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTCCCGGCGAAAGGTACTCGCACTGAt                                                  >  6:339706/1‑100 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCG  >  NC_002947/4282208‑4282500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑