Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,282,361 T→G 10.2% E290A (GAG→GCG)  PP_3753 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,282,3610TG10.2% 131.8 / 4.9 48E290A (GAG→GCG) PP_3753AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (19/24);  new base G (3/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATG  >  NC_002947/4282213‑4282505
                                                                                                                                                     |                                                                                                                                                
ggTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCt                                                                                                                                                  >  7:99722/1‑149 (MQ=255)
    gAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCg                                                                                                                                                >  1:151382/1‑147 (MQ=255)
    gAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGaa                                                                                                                                              <  1:148473/149‑1 (MQ=255)
    gAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGA‑GCGGCTGcgcg                                                                                                                                                >  7:217083/1‑147 (MQ=255)
       cGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGA‑GCGGCTGCGCGAAGGc                                                                                                                                           <  8:217083/149‑1 (MQ=255)
         cAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGA‑GCGGCTGCGCGAAGGcgc                                                                                                                                         <  7:134392/149‑3 (MQ=255)
          aGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCAt                                                                                                                                        <  2:151382/149‑1 (MQ=255)
              cGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGcc                                                                                                                                    >  2:2484/1‑149 (MQ=255)
               gACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCAt                                                                                                                                        <  8:68185/144‑1 (MQ=255)
               gACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCAt                                                                                                                                        >  7:68185/1‑144 (MQ=255)
                             ggTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATcc                                                                                                                     <  8:268709/149‑1 (MQ=255)
                             ggTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCGCGAAGGCCATGGCCCGGCGCACTTCATcc                                                                                                                     >  7:369124/1‑149 (MQ=255)
                              gTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCt                                                                                                                    <  2:79578/149‑1 (MQ=255)
                                     ccTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATTCTCAAAGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGAGCAATTCATCCTTCATct                                                                                                              >  4:260547/1‑148 (MQ=255)
                                          gCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGt                                                                                                        <  8:369124/149‑1 (MQ=255)
                                                cccTGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCTGTTCCTTAAACCCCGCCTGCTCGGCATTTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACg                                                                                                     <  2:341571/146‑1 (MQ=255)
                                                  cGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGc                                                                                                <  6:161991/149‑1 (MQ=255)
                                                      ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATc                                                                                                                      <  2:16165/123‑1 (MQ=255)
                                                      ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATc                                                                                                                      >  1:16165/1‑123 (MQ=255)
                                                      ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGcc                                                                                               >  6:259772/1‑146 (MQ=255)
                                                      ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGcc                                                                                               <  5:259772/146‑1 (MQ=255)
                                                       cACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTc                                                                                           <  8:99722/149‑1 (MQ=255)
                                                        aCTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTcc                                                                                          <  1:14982/149‑1 (MQ=255)
                                                                    cccGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGc                                                                              >  7:24689/1‑149 (MQ=255)
                                                                             aGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAg                                                                     >  5:84073/1‑149 (MQ=255)
                                                                             aGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAg                                                                     <  4:214145/149‑1 (MQ=255)
                                                                                  cTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACt                                                                >  2:71957/1‑149 (MQ=255)
                                                                                       ttCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCAc                                                           <  8:24689/149‑1 (MQ=255)
                                                                                          cTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGAt                                                                                                                >  1:44984/1‑93 (MQ=255)
                                                                                          cTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGAt                                                                                                                <  2:44984/93‑1 (MQ=255)
                                                                                           tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCg                                                                         >  4:372417/1‑131 (MQ=255)
                                                                                           tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCg                                                                         <  3:372417/131‑1 (MQ=255)
                                                                                           tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCAc                                                           <  5:137144/145‑1 (MQ=255)
                                                                                           tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCAc                                                           >  6:137144/1‑145 (MQ=255)
                                                                                           tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGAt                                                       >  7:6080/1‑149 (MQ=255)
                                                                                            gAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGAt                                                       >  1:96076/1‑149 (MQ=255)
                                                                                              aaCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATccc                                                    <  3:327149/149‑1 (MQ=255)
                                                                                               aCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTg                                                         >  5:126135/1‑143 (MQ=255)
                                                                                               aCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTg                                                         <  6:126135/143‑1 (MQ=255)
                                                                                                     ccTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTtgc                                             <  1:2484/149‑1 (MQ=255)
                                                                                                         cTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTtgctgc                                          <  8:34079/148‑1 (MQ=255)
                                                                                                            ggCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTgcgc                                        >  3:168693/1‑147 (MQ=255)
                                                                                                                     ggCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCa                             <  1:71957/149‑1 (MQ=255)
                                                                                                                                     ccTTCGA‑GCGGCTGCGCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTc              >  7:238878/1‑148 (MQ=255)
                                                                                                                                      cTTCGA‑GCGGCTGCGCGAAGGCCATGGCCCGGCGCACTTCATGCTTGATCTGCTGGTACCAACGCCCCTCCCGCTCCAACTTCCGGCGAAAGGTACAGGCACTGATCCCCTGTTGCTGAGCCAGTGCCGCCAATGTCCGCCACTGTcc             >  4:373247/1‑148 (MQ=255)
                                                                                                                                         cgc‑gCAGCCGCTCGCAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGTTACTGGCACTGATCCGCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATa          <  3:260547/148‑1 (MQ=255)
                                                                                                                                             gcAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCGAACTGGCGGCGAAAGGTACTGGCACTGAt                                                       <  5:339706/100‑1 (MQ=255)
                                                                                                                                             gcAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAgcg       >  4:211008/1‑148 (MQ=255)
                                                                                                                                             gcAGCCGCTCGAAGGCCATCGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTCCCGGCGAAAGGTACTCGCACTGAt                                                       >  6:339706/1‑100 (MQ=255)
                                                                                                                                                 ccGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATg  >  6:263713/1‑149 (MQ=255)
                                                                                                                                                     tCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTc              <  6:25766/133‑1 (MQ=255)
                                                                                                                                                     tCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGCTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTc              >  5:25766/1‑133 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGC‑GCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATG  >  NC_002947/4282213‑4282505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑