| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | 100% | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 100.0% | 95.7 / NA | 33 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/19); total (14/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 95.1 / NA | 33 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (14/19); total (14/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTC > NC_002947/1499338‑1499626 || ccGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCt < 8:53776/149‑1 (MQ=255) aGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttc > 5:30179‑M2/1‑146 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg > 1:100717‑M2/1‑141 (MQ=255) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttagcgggcacgcc < 3:137267‑M2/149‑15 (MQ=255) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccacaga > 1:277126‑M2/1‑122 (MQ=255) agCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAg > 6:80349/1‑149 (MQ=25) gCCTTAACTAAGCCTTGGCACCACAGCAGGACTGAGGCTGCAAAAGGGTGGGAGCGGGGGTGCCCGCGAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAg < 4:137267/148‑1 (MQ=18) cTGAACGACGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 5:149037/149‑1 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc > 3:124153/1‑149 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 5:80349/147‑1 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 3:151607/147‑1 (MQ=21) aCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 5:207638/148‑1 (MQ=21) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc < 8:123177/149‑1 (MQ=21) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgccccctcccac < 3:73806‑M2/109‑24 (MQ=255) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc < 7:94080/146‑1 (MQ=21) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc > 8:94080/1‑146 (MQ=21) ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 4:124153/149‑1 (MQ=21) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt < 1:109579‑M2/65‑2 (MQ=255) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt > 2:109579‑M2/1‑64 (MQ=255) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa > 8:30113/1‑149 (MQ=25) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACg < 7:306713/140‑1 (MQ=25) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACg > 8:306713/1‑140 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:342129/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttgtc < 6:374623‑M2/148‑105 (MQ=255) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa < 2:32859/105‑1 (MQ=18) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa > 1:32859/1‑105 (MQ=18) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCAATAAGCGGGCCTATAGCTACAGTGACGGCCCGTATTTTGTTGCCTCGCTAACGAATCATGACTCCAtt > 7:120785/1‑148 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttgtcg < 5:133225‑M2/148‑106 (MQ=255) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttgtcgaa < 5:101697‑M2/149‑108 (MQ=255) gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 7:100859/149‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 8:100859/1‑149 (MQ=37) ccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 4:109221/1‑148 (MQ=37) cATGGCACCGGCTTTGCCGGTGTTCG‑‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa < 1:342129/149‑1 (MQ=38) aCGGGTTTTGCGGTGTT‑TCGCGGGCAGCC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc < 1:184513/149‑1 (MQ=255) || CCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTC > NC_002947/1499338‑1499626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |