| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 92.0% | 74.1 / 0.4 | 25 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (10/13); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAT > NC_002947/1932496‑1932769 | gcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTGTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TG‑G‑cccgc < 8:13237/148‑4 (MQ=255) cTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAg < 4:158804/148‑1 (MQ=255) ttCGAAGAGGATATGGACATTCGAAAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCAAAAAATGATATTCGTCACCTGTAGGACCTGGCGTT‑C‑CCGCCAAgg > 3:184008/1‑148 (MQ=37) tttCTACTGCGGAATTGCTGGTCTAACAAAATTCGCTTTAACGGGGGGGGCGGGTTGGGGGCCGTTGGGGGGAACGCCCGCTCCTACAAAGTTTTTGGGCCCCTTGTCGGCGGGGTT‑TTCC‑CCGCGAAGGGCTGCAAGGCAGCCCCaa < 4:10032/147‑1 (MQ=255) gACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTg > 2:32879/1‑148 (MQ=255) ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCAACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCAGCAAGGCAGCCCCAAAATGTCGAca > 2:52941/1‑147 (MQ=38) gctcACTAAAATCGCAGTCACCGCGGGGGCCGCTTGGCGGCCCGTCGCGGGCAAGCCCGCTCCTACAAAGGAAATGCGTCCCCTGTAGTCGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 6:118970/147‑1 (MQ=25) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGt > 5:31903/1‑126 (MQ=255) aaaaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 6:31903/126‑1 (MQ=255) aaaCGGGGGGGCCGCTTGGCGGCCCTGCGCGGGCAAGCCCGCGCCTACAAAGCATATGCGGCCCCTGTAGGAGCGGGC‑TG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 5:175205/148‑1 (MQ=38) gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCa < 2:115153/148‑1 (MQ=255) gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa < 8:82457/148‑1 (MQ=255) cGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGCGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCCGCCCCAAATTGTCGCCTACGCGGCTCAGCGCTCCGGCAGGGTGATGTTCAGCTGCAGa > 7:48882/1‑148 (MQ=37) ggCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca < 1:32879/148‑1 (MQ=255) gCCCTTGGCGGGCAAGCCCGCTCCAACAACGTATATGCGGCCCCTGTTGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag < 3:93711/148‑1 (MQ=38) tgcgcgGGCAAGCCCGCTCCTACAAAGGATATGCGGCCCCTGTCGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGc < 1:111122/146‑1 (MQ=255) gCAAGCCCGGTCCTCCAAAGGTTTTGCGTCCCGTGTAGGTGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtt < 1:52941/148‑1 (MQ=38) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt > 2:10095/1‑148 (MQ=255) aGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 3:155139/148‑1 (MQ=255) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 1:57338/1‑148 (MQ=255) cAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 2:3182/1‑147 (MQ=255) ggATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca > 6:196588/1‑148 (MQ=255) gATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcat < 2:64953/148‑1 (MQ=255) gATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTACCCTGGTTTTCCAGCTCGATATGcaca > 3:8504/1‑147 (MQ=255) tatGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca < 1:10095/148‑1 (MQ=255) tGTAGGAGCGGGC‑TT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 4:147949/1‑148 (MQ=255) | GCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAT > NC_002947/1932496‑1932769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |