Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,580,997 T→C K134E (AAG→GAG)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,580,9970TC97.4% 128.7 / NA 39K134E (AAG→GAG) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (20/18);  minor base A (0/1);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCATGCTG  >  NC_002947/1580861‑1581139
                                                                                                                                        |                                                                                                                                              
ccGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTgg                                                                                                                                     <  2:204963/148‑1 (MQ=255)
 cGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGt                                                                                                                                    <  2:48591/148‑1 (MQ=255)
   aaCACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGa                                                                                                                                  >  2:136477/1‑148 (MQ=255)
            aaGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATa                                                                                                                          <  7:110524/147‑1 (MQ=255)
            aaGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATAc                                                                                                                         <  3:151815/148‑1 (MQ=255)
                 aCCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTtgc                                                                                                                    >  2:190375/1‑148 (MQ=255)
                   cTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTtgctg                                                                                                                  >  4:155106/1‑148 (MQ=255)
                   cTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTtgctg                                                                                                                  >  1:94786/1‑148 (MQ=255)
                                 cgcgAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGATGCTGCACTTCCTGCg                                                                                                    >  5:103826/1‑148 (MQ=255)
                                    gagaTCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGgca                                                                                                 <  3:15367/148‑1 (MQ=255)
                                     agaTCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGgca                                                                                                 >  8:15879/1‑147 (MQ=255)
                                            gTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTGCTGCGGCAGCAGCggg                                                                                         <  2:31200/148‑1 (MQ=255)
                                            gTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCggg                                                                                         <  7:179159/148‑1 (MQ=255)
                                               gtGCATGTTCGAGACGATTTAGTTGGCCAGGTCGTCCTTTGTCATGCTGCCGTCTTCGGAGACGAGGCCGATCACGAACATGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTg                                                                                      <  2:172442/147‑1 (MQ=255)
                                                tGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTgg                                                                                     >  2:101280/1‑148 (MQ=255)
                                                tGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACAAGGCCGATCACCAGCAGGAAGTTCTCCCCTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTgg                                                                                     >  7:72295/1‑148 (MQ=255)
                                                         gagaCGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTc                                                                             <  3:155106/147‑1 (MQ=255)
                                                              gATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCa                                                                                  >  2:148611/1‑137 (MQ=255)
                                                              gATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTACTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTg                                                                       >  3:30807/1‑148 (MQ=255)
                                                               atatAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAg                                                                                 <  1:148611/137‑1 (MQ=255)
                                                                 ataGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTc                                                                    <  6:34163/148‑1 (MQ=255)
                                                                          cAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTctgtacctgt                                                           >  4:101077/1‑148 (MQ=255)
                                                                                 tcCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTgcg                                                    >  7:180185/1‑148 (MQ=255)
                                                                                           tgctgcCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCggg                                          <  1:101280/148‑1 (MQ=255)
                                                                                                ccGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGtt                                       >  5:182063/1‑146 (MQ=255)
                                                                                                 cGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTg                                      <  6:182063/146‑1 (MQ=255)
                                                                                                 cGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTg                                      <  8:146805/146‑1 (MQ=255)
                                                                                                     ttCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGccc                                >  8:112466/1‑148 (MQ=255)
                                                                                                          agaCCAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGtt                            >  5:139462/1‑147 (MQ=255)
                                                                                                              cAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAg                       <  3:101077/148‑1 (MQ=255)
                                                                                                              cAGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAg                       >  6:95937/1‑148 (MQ=255)
                                                                                                               aGGCCGATCACCAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAgg                      >  8:167648/1‑148 (MQ=255)
                                                                                                                         ccAGCAGGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTaa             <  2:94786/147‑1 (MQ=255)
                                                                                                                          cagcagGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATg           >  1:31385/1‑148 (MQ=255)
                                                                                                                          cagcagGAAGTTCTACACTGCTTTGGTGACGCTGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCTGTGTCGTGGTTGGTGCCCTGTTCGAAGGTGGAGGTAATg           <  6:103826/148‑1 (MQ=255)
                                                                                                                             cagGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTc        <  1:136477/148‑1 (MQ=255)
                                                                                                                              agGAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCa       >  5:88325/1‑148 (MQ=255)
                                                                                                                                gAAGTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCAtg     >  2:8965/1‑148 (MQ=255)
                                                                                                                                   gTTCTCCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCAtgctg  <  1:190375/148‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                              
CCGAACACCTGGAAGTCACCTACACCCGCGGTACGCGAGATCGGGTCCTGCATGTTCGAGACGATATAGTTGGCCAGGTCGTCCTTGGTCATGCTGCCGTCTTCGGAGACCAGGCCGATCACCAGCAGGAAGTTCTTCACTGCTTTGGTGACGCGGATACCTTGCTGCTGCACTTCCTGCGGCAGCAGCGGGGTGGCCAGGTTCAGCTTGTTCTGTACCTGTACCTGCGCGGTGTCGGGGTTGGTGCCCTGTTCGAAGGTGGCGGTAATGGTCATGCTG  >  NC_002947/1580861‑1581139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑