Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,029,411	G→T	G384V (GGG→GTG)	wbpM →	polysaccharide biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,029,411	0	G	T	100.0%	84.0 / NA	26	G384V (GGG→GTG)	wbpM	polysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/14);  total (12/14)

GAGCTTTGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGGGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGTT  >  NC_002947/2029266‑2029546                                                                                                                                                  |                                                                                                                                        gAGCTTTGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGgtgt                                                                                                                                       >  2:50498/1‑148 (MQ=255)  aGCTTTGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTc                                                                                                                                      <  1:50498/148‑1 (MQ=255)  aGCTTTGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTc                                                                                                                                      >  8:19117/1‑148 (MQ=255)     tttGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGCCCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGt                                                                                                                                   >  6:30307/1‑148 (MQ=255)         ccgccAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACg                                                                                                                                >  3:74137/1‑147 (MQ=255)          cgccAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCAGGTGTCGGTACGCa                                                                                                                              >  3:160114/1‑148 (MQ=255)                aTCATATGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATcagc                                                                                                                        <  5:121259/148‑1 (MQ=255)                 tCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATcagca                                                                                                                       >  7:58311/1‑148 (MQ=255)                  cATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATcagcag                                                                                                                      <  5:97762/148‑1 (MQ=255)                                   cGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTAt                                                                                                     <  1:65946/148‑1 (MQ=255)                                      cATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTc                                                                                                  <  6:47761/148‑1 (MQ=255)                                            tgctgTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACtt                                                                                             <  7:146071/147‑1 (MQ=255)                                                    gACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCATCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTa                                                                                     <  8:90359/147‑1 (MQ=255)                                                               gTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGGCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTAc                                                                         <  6:95765/148‑1 (MQ=255)                                                                            gCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCggcggc                                                              <  7:19117/146‑1 (MQ=255)                                                                                tCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACaa                                                        >  6:191562/1‑148 (MQ=255)                                                                                tCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACaa                                                        >  1:80343/1‑148 (MQ=255)                                                                                   tCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACa                                                     >  1:166262/1‑148 (MQ=255)                                                                                          gCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGccc                                              >  7:1718/1‑148 (MQ=255)                                                                                           cTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGCGCCCa                                             <  8:58311/148‑1 (MQ=255)                                                                                                gCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGtt                                        <  2:166262/148‑1 (MQ=255)                                                                                                  aaCGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGa                                      <  8:119973/148‑1 (MQ=255)                                                                                                     gcgTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCCGCAGCATCGTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTCCCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGc                                    >  5:155466/1‑147 (MQ=255)                                                                                                       gTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGcaca                                 >  8:14200/1‑148 (MQ=255)                                                                                                                      cTGCCGGTGCGACTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGta                  <  3:25748/148‑1 (MQ=255)                                                                                                                                 aCTGCTGCCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAAt       <  4:160114/148‑1 (MQ=255)                                                                                                                                      tgcCCATTCTGGTGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGtt  >  8:184647/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                        GAGCTTTGCCGCCAGATCATAGGGCTTGGGCCAACGACATTGCTGCTGTTCGACCACAGCGAGTACAACCTCTACGCTATCCTCAGTGAGCTGGAGCAACGCGTTGCGCGCGAGTCGCTGCCGGTGCGACTGCTGCCCATTCTGGGGTCGGTACGCAATCAGCAGCATCTTGCCCACGTTATGTCCACTTGGCGGGTAGCCACCGTCTACCACGCGGCGGCTTACAAACATGTGCCCATGGTTGAGCACAACATGGCGGAGGGTATACTCAACAATGTGTT  >  NC_002947/2029266‑2029546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑