Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,987,522	G→T	G202C (GGT→TGT)	kefB‑III →	glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,987,522	0	G	T	100.0%	127.5 / NA	38	G202C (GGT→TGT)	kefB‑III	glutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/19);  total (19/19)

TGTTCCAGGACATCGCCGCGATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCGGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATGGAAGAAGCGGGTATCTCCAT  >  NC_002947/5987385‑5987649                                                                                                                                          |                                                                                                                                tgttCCAGGACATCGCCGCGATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTAc                                                                                                                         <  3:135409/146‑1 (MQ=255)    tCCAGGACATCGCCGCGATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGtt                                                                                                                    <  1:118208/148‑1 (MQ=255)    tCCAGGACATCGCCGCGATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGtt                                                                                                                    <  3:106547/148‑1 (MQ=255)     ccAGGACATCGCCGGGTTCCCGCTGATCGCCTGTGTAGCGCGGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGtt                                                                                                                    <  2:67528/147‑1 (MQ=255)     ccAGGACATCGCCGCGATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTg                                                                                                                   >  2:168789/1‑148 (MQ=255)         gACATCGCCGCGATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGcc                                                                                                               >  2:206472/1‑148 (MQ=255)             tCGCCGCGATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGgt                                                                                                           <  3:152501/148‑1 (MQ=255)              cgccgcGATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGgtg                                                                                                          >  3:189647/1‑148 (MQ=255)                 cgcgATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGt                                                                                                                               >  8:158682/1‑124 (MQ=255)                 cgcgATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTc                                                                                                       <  1:95240/148‑1 (MQ=255)                  gcgATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTc                                                                                                                              <  7:158682/124‑1 (MQ=255)                  gcgATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGAGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTcc                                                                                                      <  7:162661/148‑1 (MQ=255)                      tCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCAc                                                                                                  >  8:99434/1‑148 (MQ=255)                          gCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTg                                                                                              <  4:55797/148‑1 (MQ=255)                          gCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGAAACACGGCCTGAAGGTATTAGCCAGCATCGCCGTGCTGATCATCGGCTCTCGCTACCTGTTGCGCCCGGTCTTCCGCACCGTg                                                                                              >  8:161213/1‑148 (MQ=255)                               tCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGccag                                                                                         >  3:42700/1‑146 (MQ=255)                                   cTTGGTACCGCTGCTGGCCGCCAGGGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACg                                                                                     <  7:108414/148‑1 (MQ=255)                                    ttGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACgg                                                                                    <  6:28042/148‑1 (MQ=255)                                                 tgGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCGGCAACACGGCGTGAAGTTATTCGCCAGCAGCGCCGTGGTGATGATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGaa                                                                         <  7:136570/146‑1 (MQ=255)                                                        cAGTGGCCCAGACACCAGCCATGGCGACAGCCTGCATCACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTAGCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTagt                                                                <  7:161213/148‑3 (MQ=255)                                                           tGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGcc                                                             >  1:57744/1‑148 (MQ=255)                                                                     acCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCActgc                                                   >  6:103463/1‑148 (MQ=255)                                                                       cAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCActgctg                                                 >  1:98893/1‑148 (MQ=255)                                                                                 gACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCg                                       <  6:105842/148‑1 (MQ=255)                                                                                     gCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCAc                                   <  1:1019/148‑1 (MQ=255)                                                                                         gCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGcc                               >  3:185812/1‑148 (MQ=255)                                                                                            acacGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTgg                            <  7:74968/148‑1 (MQ=255)                                                                                             cacGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGc                           <  3:189287/148‑1 (MQ=255)                                                                                             cacGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGc                           <  1:168789/148‑1 (MQ=255)                                                                                                     gAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATg                      >  1:19703/1‑145 (MQ=255)                                                                                                      aaGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATGgaa                   >  5:5459/1‑147 (MQ=255)                                                                                                      aaGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATGgaa                   >  8:112711/1‑147 (MQ=255)                                                                                                      aaGCTATTCGCCAGCATCGCCGTGGTCATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTACCGCACCGTGGCCCCCACGCGCCTGCCGCAACTGTCTACTGCCACCGCACTGC‑AGTACTGATCGCCACCGCCACGCAGATGgaa                   >  4:148373/1‑146 (MQ=255)                                                                                                       aGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATGgaa                   >  2:94990/1‑146 (MQ=255)                                                                                                         gTATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATGGaagaag               >  6:94280/1‑148 (MQ=255)                                                                                                          tATTCGCCAGCATCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATGGAAGAAGc              >  7:95446/1‑148 (MQ=255)                                                                                                                     aTCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATGGAAGAAGCGGGTATCTCCa   >  5:5661/1‑148 (MQ=255)                                                                                                                      tCGCCGTGGTGATCATCGGCTGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATGGAAGAAGCGGGTATCTCCAt  <  4:189647/148‑1 (MQ=255)                                                                                                                                          |                                                                                                                                TGTTCCAGGACATCGCCGCGATCCCGCTGATCGCCTTGGTACCGCTGCTGGCCGCCAGTGGCCCAGACACCAGCCAAGGCGACAGCCTGCAACACGGCCTGAAGGTATTCGCCAGCATCGCCGTGGTGATCATCGGCGGTCGCTACCTGTTGCGCCCGGTGTTCCGCACCGTGGCCCGCACGGGCCTGCCGGAAGTGTCTACTGCCACCGCACTGCTGGTAGTGATCGGCACCGCCTGGCTGATGGAAGAAGCGGGTATCTCCAT  >  NC_002947/5987385‑5987649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑