Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	838,806	C→T	A298T (GCG→ACG)	prs ←	ribose‑phosphate pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	838,806	0	C	T	100.0%	116.6 / NA	35	A298T (GCG→ACG)	prs	ribose‑phosphate pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/22);  total (13/22)

CCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACTTCTCGATGTTCTCGATTGCGCGGCCCGACAGGACAGG  >  NC_002947/838676‑838950                                                                                                                                   |                                                                                                                                                 ccAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTg                                                                                                                                 <  5:138932/148‑1 (MQ=255) ccAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTg                                                                                                                                 <  5:32812/148‑1 (MQ=255)         aaCGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATcc                                                                                                                         >  6:113491/1‑148 (MQ=255)                    cAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGAt                                                                                                              >  5:161176/1‑148 (MQ=255)                     aaaaaaGCCCCGCCCCAACACAGGTT‑GGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATAc                                                                                                            >  7:158907/1‑148 (MQ=255)                        aaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACgg                                                                                                          <  6:161176/148‑1 (MQ=255)                        aaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACgg                                                                                                          <  1:68172/148‑1 (MQ=255)                            ccccgccccAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTc                                                                                                        >  1:54921/1‑146 (MQ=255)                             cccgccccAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTcaca                                                                                                     >  3:108180/1‑148 (MQ=255)                             cccgccccAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTcaca                                                                                                     <  1:130517/148‑1 (MQ=255)                               cgccccAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAgg                                                                                                   <  5:85921/148‑1 (MQ=255)                                    cAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGa                                                                                              <  4:108180/148‑1 (MQ=255)                                    cAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGa                                                                                              <  1:62991/148‑1 (MQ=255)                                    cAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGa                                                                                              <  5:199289/148‑1 (MQ=255)                                          aGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCg                                                                                        <  2:105647/148‑1 (MQ=255)                                                         gcTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACg                                                                         <  6:166736/148‑1 (MQ=255)                                                         gcTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACg                                                                         <  2:54921/148‑1 (MQ=255)                                                            tCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCTGCGGCAGACAGCGGAACGgtg                                                                      >  5:788/1‑148 (MQ=255)                                                              acaTCAACTCGTGTCCCGCTTAGCGGAACATCGCGCTTATGGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGgtgt                                                                     <  6:113575/147‑1 (MQ=255)                                                                aTCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTTCCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTgg                                                                  >  7:64966/1‑148 (MQ=255)                                                                           tCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGgtg                                                                      >  3:173757/1‑133 (MQ=255)                                                                            cccGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGgtgt                                                                     <  4:173757/133‑1 (MQ=255)                                                                             ccGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTc                                                     <  5:127657/148‑1 (MQ=255)                                                                              cGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCg                                                    >  4:159803/1‑148 (MQ=255)                                                                                    gCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAg                                               <  4:47689/147‑1 (MQ=255)                                                                                           aTCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACt                                       >  5:51217/1‑148 (MQ=255)                                                                                            tCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTc                                                  >  5:86942/1‑136 (MQ=255)                                                                                             cgcgCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTcc                                                 <  6:86942/136‑1 (MQ=255)                                                                                             cgcgCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACTtc                                     <  2:27468/148‑1 (MQ=255)                                                                                               cgcTGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACTtct                                    <  7:22084/147‑1 (MQ=255)                                                                                                  tGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACTTCTCGAt                                >  4:114695/1‑148 (MQ=255)                                                                                                  tGATCGATTCTTCGTTGCTGATGCGGCGTACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACTTCTCGAt                                <  2:105790/148‑1 (MQ=255)                                                                                                                              tACCGTTTCAGCGACAACCGGTGTGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACTTCTCGATGTTCTCGATTGCGCGGCCCgacaggaca    <  4:64752/148‑1 (MQ=255)                                                                                                                              tACCGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACTTCTCGATGTTCTCGATTGCGCGGCCCgacaggaca    <  2:172656/148‑1 (MQ=255)                                                                                                                                ccGTTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACTTCTCGATGTTCTCGATTGCGCGGCCCGacaggacagg  >  1:23910/1‑148 (MQ=255)                                                                                                                                   |                                                                                                                                                 CCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCAGTACCGACTTCTCGATGTTCTCGATTGCGCGGCCCGACAGGACAGG  >  NC_002947/838676‑838950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑