Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,932,640 +C intergenic (‑107/+43) rluA ← / ← minE bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,932,6381.C96.7% 195.4 / ‑0.4 60intergenic (‑105/+45)rluA/minEbifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (31/27);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT  >  NC_002947/1932491‑1932779
                                                                                                                                                     |                                                                                                                                               
tGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑c                                                                                                                                                <  7:274472/149‑1 (MQ=255)
          ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGCTATGCGTCCCCTGGAGGAGCGGGCTT‑GCCCGCGAAggg                                                                                                                                       >  2:305304/1‑149 (MQ=38)
             gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTg                                                                                                                                    >  8:85403/1‑149 (MQ=255)
                        ggACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGcc‑c                                                                                                                        >  3:43721/1‑149 (MQ=255)
                        ggACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGcc‑c                                                                                                                        >  2:214889/1‑149 (MQ=255)
                                 accgCGGCATTGCAGGTCGCACGAAAATCGCAGTAACCGCTGGGGCCGCTTGGCGGCCCGTCGCGGGCACGCCCGCTCCTACAATGGATATGCGTCCCCTGGAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTc                                                                                                              <  1:305304/146‑1 (MQ=37)
                                 aCAGCAGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTc                                                                                                              >  4:128346/1‑149 (MQ=255)
                                    gCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGACCGCGCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAACGGCTGCAAGGCAGAC‑C‑AAAATTGTCGa                                                                                                            >  8:177181/1‑148 (MQ=38)
                                         aTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTAcgc                                                                                                      >  7:92777/1‑149 (MQ=255)
                                              aGGTCTCACGAAAATAGCATTAACCGCTGGGGCCGCTTTGCGGCCGTTCGCGGGCAAGCCGGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGAt                                                                                                  <  4:43721/148‑1 (MQ=38)
                                                gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCACCTACAAAGGATAATGGTCCCCGTTAGGACCGGGCTT‑GCCCGCGAACGGCTGGCAGGCCGCC‑C‑CAAAATGTCGACAACGCGGATAAg                                                                                               >  1:140003/1‑149 (MQ=25)
                                                     aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTAc                                                                                                        <  1:240892/135‑1 (MQ=255)
                                                     aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTAc                                                                                                        >  2:240892/1‑135 (MQ=255)
                                                     aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATc                                                                                          >  2:72798/1‑149 (MQ=255)
                                                     aCGAAAATAGCAGTAAACGCTCGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTACGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTCTCGACTACGCGGATCAGCGCTc                                                                                          >  7:271156/1‑149 (MQ=37)
                                                      cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATcc                                                                                         <  3:128346/149‑1 (MQ=255)
                                                                     ccGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTAAAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc                                                                          <  7:177181/148‑1 (MQ=255)
                                                                     aCGCGGGGGCCGTTTTGCGGCCCTTCGGGGGCAACCCCGCTCCTACAACGTATATGCGCCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc                                                                          <  2:376222/149‑1 (MQ=38)
                                                                       gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGGAAGGCAGCC‑C‑CAAATTGTCGACTAcgc                                                                                                      >  3:350607/1‑119 (MQ=255)
                                                                       gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg                                                                        >  8:194838/1‑149 (MQ=255)
                                                                        cTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTGCCCTGTAGGAGCGGGCTTGG‑CCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGc                                                                       >  7:344954/1‑149 (MQ=255)
                                                                            ggCCGCTTTGCGGCCCGTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa                                                                   <  3:158869/149‑1 (MQ=255)
                                                                             gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTGCTACAAAGGATATGCGTGCCCTGTAGGAGCGGGCTTGG‑CCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGt                                                                                 >  3:174930/1‑134 (MQ=255)
                                                                                       ggCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg                                                                                   >  4:341438/1‑122 (MQ=255)
                                                                                       ggCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg                                                                                   <  3:341438/122‑1 (MQ=255)
                                                                                           cTTCGCGGGCAAGCCCGCTCCTTAAAAGTAAATGGCTCCCCTTTATGAGGGGGCTT‑GCCCGGGAATGGCTGCAAGGCATCCTCGCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCt                                                      <  1:114687/149‑1 (MQ=255)
                                                                                               gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGGTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg                                                >  1:159440/1‑149 (MQ=255)
                                                                                               gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg                                                <  8:92777/149‑1 (MQ=255)
                                                                                                cgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTTGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTgg                                               <  1:214889/149‑1 (MQ=255)
                                                                                                     aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                                          <  7:194838/149‑1 (MQ=255)
                                                                                                        cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt                                                 >  4:253800/1‑139 (MQ=255)
                                                                                                        cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt                                                 <  3:253800/139‑1 (MQ=255)
                                                                                                         ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTc                                                                                                              >  3:359413/1‑77 (MQ=255)
                                                                                                         ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTc                                                                                                              <  4:359413/77‑1 (MQ=255)
                                                                                                               cTACAAAGGATATGCGTCCCCTGTAGGTGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata                                <  7:177415/149‑1 (MQ=255)
                                                                                                                 aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg                              >  4:78997/1‑149 (MQ=255)
                                                                                                                 aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGCGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg                              >  1:78157/1‑149 (MQ=255)
                                                                                                                  cAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAACAGCTGCCCTGGTTTTCCAGCTCGATATg                              >  7:340094/1‑148 (MQ=255)
                                                                                                                  cAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCACAATCGAGCAGATGCCCTGGTTTTCCAGCTCGATATg                              >  7:13789/1‑148 (MQ=255)
                                                                                                                   aaaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc                                                              >  5:109421/1‑115 (MQ=255)
                                                                                                                   aaaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc                                                              <  6:109421/115‑1 (MQ=255)
                                                                                                                    aaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca                            >  7:94035/1‑148 (MQ=255)
                                                                                                                     aGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcaca                          <  1:72798/149‑1 (MQ=255)
                                                                                                                        atatGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGGAGCC‑G‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca                       <  6:386098/149‑1 (MQ=255)
                                                                                                                           tGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc                     <  2:78157/148‑1 (MQ=255)
                                                                                                                            gCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt                   >  1:76652/1‑149 (MQ=255)
                                                                                                                             ggTCCGCTGTTGTAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt                   <  8:271156/147‑1 (MQ=39)
                                                                                                                             cGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAACAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt                  <  8:340094/149‑1 (MQ=255)
                                                                                                                               tCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc                >  2:374178/1‑149 (MQ=255)
                                                                                                                                 cccTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc               >  3:83107/1‑148 (MQ=255)
                                                                                                                                  ccTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac                           <  6:123555/135‑1 (MQ=255)
                                                                                                                                  ccTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac                           >  5:123555/1‑135 (MQ=255)
                                                                                                                                   cTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc                                      <  1:345804/123‑1 (MQ=255)
                                                                                                                                   cTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc                                      >  2:345804/1‑123 (MQ=255)
                                                                                                                                      tcggAGCGGGGTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt         <  2:159440/147‑1 (MQ=255)
                                                                                                                                       aGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt         <  3:78997/148‑1 (MQ=255)
                                                                                                                                         gAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCGGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca       >  6:140966/1‑148 (MQ=255)
                                                                                                                                          cgggggCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca     <  2:140003/146‑1 (MQ=255)
                                                                                                                                             gggCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACatat  >  5:86835/1‑149 (MQ=255)
                                                                                                                                               gCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc                                    >  4:382082/1‑113 (MQ=255)
                                                                                                                                               gCTT‑GCCCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc                                    <  3:382082/113‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
TGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCC‑C‑CAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT  >  NC_002947/1932491‑1932779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑