| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,796,617 | 0 | G | C | 34.0% | 56.6 / 45.2 | 53 | intergenic (‑175/+43) | pvdH/PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base G (17/18); new base C (9/9); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGC > NC_002947/4796474‑4796755 | gCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGAGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGc < 8:312307/148‑1 (MQ=255) ggTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg > 5:157161/1‑149 (MQ=255) gTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGt < 3:222188/149‑1 (MQ=38) gACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCa < 3:40591/149‑1 (MQ=255) tGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcg < 6:183685/149‑1 (MQ=255) gTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc > 8:117693/1‑147 (MQ=255) gTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc < 7:117693/147‑1 (MQ=255) tCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGTAGTATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCcgc < 4:280982/142‑1 (MQ=37) tCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGagatcg > 3:280982/1‑144 (MQ=37) tCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAc < 2:60004/138‑1 (MQ=37) tCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAc > 1:60004/1‑138 (MQ=37) tCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc > 6:366915/1‑147 (MQ=255) gCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAAACCGCCCCTTGGAGCGGGTTCACCCGCGAACCGGccccgaacg > 4:210800/1‑142 (MQ=255) aaGCGGATGGGCGGTTATCGCTTTGTAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑c < 2:58518/149‑1 (MQ=255) aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCgg < 2:175995/121‑1 (MQ=255) aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCgg > 1:175995/1‑121 (MQ=255) gCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTAGGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGTAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATc < 4:291569/149‑1 (MQ=255) gtttATCGCTTTGGAAACGCTTGTGTGGGGGGTGTATTTTGGGGTTGTTACGCTATTTTATTTTTGTCTGTGCTGGCGTCTTCGGGGGTAAACCCGCCCGTAGGAGCGGGTTCACCGGCGAAGAGGCCAGCACAGG‑CGATAAACATCa < 6:26530/146‑1 (MQ=38) gTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGCGCCCAGCACAGG‑CGATAAACaaaaat > 6:210627/1‑144 (MQ=255) tATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGAGAAGAGGCCAGCACAGG‑CGATAAACATGaaag > 5:26530/1‑146 (MQ=255) cgttttGGTATCGGTTGAGTGGGGGGAGTATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTGGCGGTTAAACCCGCCCGTAGGAGCGGGTTCACCGGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGccc < 4:187322/145‑1 (MQ=38) gAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGAcgc < 5:366915/148‑1 (MQ=255) aCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTGCCCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAgg > 3:243088/1‑141 (MQ=255) aCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCg > 4:282281/1‑149 (MQ=255) tGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCCGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGc < 6:157161/148‑1 (MQ=255) gggggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCg > 6:191606/1‑81 (MQ=255) gggggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCg < 5:191606/81‑1 (MQ=255) gaggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACaaaa < 7:162643/149‑1 (MQ=255) ggaTTTAGAGGTTGTTACGCTATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAAcc < 6:360964/149‑1 (MQ=255) aTTTAGAGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAACTGGTTAACCCGCGAAGAGGACAGCACAGG‑CGATAAACATCAATGCCCAGGACGCCCCCGTACCTGCAACAAAACCCg > 3:360283/1‑149 (MQ=38) tttAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACAAAAATGCCCAGGACGCGCCGGTACCTGCAACAAAAcccc > 4:140679/1‑147 (MQ=255) gttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCa < 3:140679/148‑1 (MQ=255) ttttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAAccc > 3:319490/1‑128 (MQ=255) ttttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAAccc < 4:319490/128‑1 (MQ=255) tatcgccTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGA‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAgc > 5:148976/7‑149 (MQ=21) gtgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTa > 3:312877/1‑149 (MQ=255) gCCTCATCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAACCATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGca < 3:210800/149‑1 (MQ=255) gcgGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTg > 5:360203/1‑149 (MQ=255) gtgaaCCCGCTCCTACGGGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAgc < 1:254121/120‑1 (MQ=37) gtgaaCCCGCTCCTACGGGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAgc > 2:254121/4‑123 (MQ=37) gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 1:319910/1‑148 (MQ=255) tgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAAcc < 1:79990/93‑1 (MQ=18) tgaaCCCGCTCATACGGGCGGGTTTACACGCGAAGAGGCAAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAAcc > 2:79990/3‑94 (MQ=255) aaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt > 4:289304/1‑114 (MQ=37) aaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt < 3:289304/114‑1 (MQ=37) aaCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATc > 3:254037/1‑149 (MQ=255) cccgctccTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGcc > 2:45637/1‑98 (MQ=25) cccgctccTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGcc < 1:45637/98‑1 (MQ=25) cccgctccTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc > 2:300918/1‑149 (MQ=38) cccgctccTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGA‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc < 6:148976/149‑1 (MQ=38) cccgcccgTAGGAGCGGGTTCACCGGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc < 4:360283/149‑1 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAg > 7:32366/1‑121 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAg < 8:32366/121‑1 (MQ=255) | GCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGC > NC_002947/4796474‑4796755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |