Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,740,471	0	A	T	30.9%	39.0 / 30.9	44	intergenic (+36/+35)	hutF/PP_5037	formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (12/17);  new base T (7/6);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.18e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGT  >  NC_002947/5740323‑5740617                                                                                                                                                     |                                                                                                                                                   aGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCaa                                                                                                                                                    <  6:268317/149‑1 (MQ=255) aGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCaa                                                                                                                                                    <  8:309330/149‑1 (MQ=255)   gTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGc                                                                                                                                                  >  6:277363/1‑149 (MQ=255)      cgcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCcg                                                                                                                                               <  5:277363/149‑1 (MQ=255)             tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCTAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGGTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAgg                                                                                                                                         <  5:234050/148‑1 (MQ=255)             tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAggg                                                                                                                                        <  4:376313/149‑1 (MQ=255)             tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGGGCTTGCACGCGAAggg                                                                                                                                        <  1:78763/149‑1 (MQ=255)               aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCATCCCATAGCGTGCAATCCCCCCAAGGGtt                                                                                                                                      >  6:160261/1‑149 (MQ=255)                   tGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaa                                                                                                                                  <  4:139194/149‑1 (MQ=255)                     aaTGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGGGCTTGCACGCGAAGGGCTgcaac                                                                                                                                 <  6:234172/148‑1 (MQ=38)                     aaTGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAGGGCTgcaac                                                                                                                                 <  6:52199/148‑1 (MQ=38)                     aaTGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAGGGCTgcaac                                                                                                                                 >  5:52199/1‑148 (MQ=38)                             aTGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGGTTGACCCCATCGCGGGGCACGCCGCGCAGGGTTGGCACGCCAcccc                                                                                                                         >  1:293066/1‑148 (MQ=255)                                      gcgcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTCCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCa                                                                                                               <  1:213201/149‑1 (MQ=255)                                       cgcCCGGGGCGGCTTGCCCAGGAATTTTAATGGGGGCGGGTGTTTGCAATGGTTTTGCCCCATTTTTGGGGCTCAGCTTACGGGGTTTGCTTTGCCGCCCTTCGCGTGAACGCCCGCTATGGTTTGAAACGCAACCCCAAAAAACTCa                                                                                                               <  4:210036/148‑1 (MQ=255)                                        gcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGGCTTTGAGCCCATAGGGTGGAAACCCCCGGAGGGGTGGAACGGCACCCCaaaaaaatacag                                                                                                             >  7:331801/1‑143 (MQ=255)                                              ggcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaacgcaac                                                                                                                            <  7:20956/128‑1 (MQ=255)                                              ggcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCGTCGCGTGGAAGCCCGCGAAGGGTTggaacccaac                                                                                                                            >  8:20956/1‑128 (MQ=255)                                                        ccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg                                                                                             <  5:160261/149‑1 (MQ=255)                                                        ccAGGAAGATCAACGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTCAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGCAACGCAACCCCAAAAAACTCa                                                                                                               <  7:13957/131‑1 (MQ=255)                                                                 tGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCa                                                                                                               <  5:161157/122‑1 (MQ=255)                                                                  gAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAGGGCTgcaac                                                                                                                                 >  7:4680/1‑103 (MQ=21)                                                                  gAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAGGGCTgcaac                                                                                                                                 <  8:4680/103‑1 (MQ=21)                                                                                                ccccAGCTTTGGGGCTGAGCGTACCGGGTTTGCGTTCCACCCCTGCGCGGCCACGCCCGCTATGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAc                                                     <  1:354319/147‑1 (MQ=255)                                                                                                 gcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCATAGCGTGCAACCCCCCCAACGGTTGGCACCCCAACCCAAAAAAAAAAGTGCAATTTTTATATTTTATGATAGGGCGCCCCCCACATCAAGCGGCAGGTGTCaaaac                                                    >  2:262527/1‑145 (MQ=255)                                                                                                                 aGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCTGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGcc                                      <  1:262527/147‑1 (MQ=255)                                                                                                                 aGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                         <  7:2450/144‑1 (MQ=255)                                                                                                                  gCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt                                    <  7:228287/148‑1 (MQ=255)                                                                                                                       ccGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcag                              <  7:217016/149‑1 (MQ=255)                                                                                                                                  ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCaa                                                                     <  2:341752/99‑1 (MQ=255)                                                                                                                                  ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCaa                                                                     >  1:341752/1‑99 (MQ=255)                                                                                                                                     cAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCt                >  8:89039/1‑149 (MQ=255)                                                                                                                                      aacccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAc                                                     >  4:131532/3‑111 (MQ=37)                                                                                                                                      aacccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc                     >  2:271447/3‑143 (MQ=255)                                                                                                                                      aacccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc               >  2:54807/3‑149 (MQ=255)                                                                                                                                      aacccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCCGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc                     <  1:271447/141‑1 (MQ=38)                                                                                                                                      aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTg                                                              <  2:339881/102‑1 (MQ=255)                                                                                                                                      aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTg                                                              >  1:339881/1‑102 (MQ=255)                                                                                                                                       acccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGCCGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc              >  7:86636/2‑149 (MQ=39)                                                                                                                                        cccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc              >  5:48769/1‑148 (MQ=255)                                                                                                                                          cTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg           >  8:111884/1‑149 (MQ=255)                                                                                                                                          cTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                    >  2:312200/1‑140 (MQ=39)                                                                                                                                          cTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                    <  1:312200/140‑1 (MQ=39)                                                                                                                                                   cAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCAttttt                                                                                                     <  6:2820/50‑1 (MQ=255)                                                                                                                                                   cAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCAttttt                                                                                                     >  5:2820/1‑50 (MQ=255)                                                                                                                                                   cAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGTGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAgt  <  7:38846/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGT  >  NC_002947/5740323‑5740617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑