| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 2,921,460 | G→A | G3494R (GGG→AGG) | PP_2561 → | hemolysin‑type calcium‑binding bacteriocin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,921,460 | 0 | G | A | 97.3% | 113.9 / NA | 37 | G3494R (GGG→AGG) | PP_2561 | hemolysin‑type calcium‑binding bacteriocin |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (19/17); minor base T (0/1); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC > NC_002947/2921322‑2921600 | cagggggAGGCCGGTAACGACAACCTGAACGGGGCCGCTGGCGATGACAGCCTGGATGGCGGCAAGCGCAATGACACCCGGCAGGGCGGCGATGGTAACGACACAATGTTCGGCGACGTGGGTACCGACGCCCGGATCAGGGGGGCg‑gg > 4:302173/3‑149 (MQ=255) ccGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGAGTTc < 5:103620/149‑1 (MQ=255) ggcacccaCAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCATCGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCTGGGGGGCC‑GGCAACGACTTCCt < 3:302173/142‑1 (MQ=255) ggTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCt > 8:317247/1‑149 (MQ=255) aCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAAc > 6:335391/1‑149 (MQ=255) cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCgg > 3:368208/1‑148 (MQ=255) gggggCGCTGGCGATGACAGCCTGGATGGCGGCAATGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTaa > 2:144318/1‑149 (MQ=255) ggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGa < 4:368208/149‑1 (MQ=255) gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtg < 1:168080/149‑1 (MQ=255) gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtg > 8:12710/1‑149 (MQ=255) gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTCCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggt < 5:335391/148‑1 (MQ=255) gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtg > 4:151414/1‑147 (MQ=255) gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtg < 3:151414/147‑1 (MQ=255) gCCTGGATGGCGGCAATGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGtgggtggg < 1:144318/149‑1 (MQ=255) gATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCgg < 7:209065/149‑1 (MQ=255) ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGCCACCCTGATCAGGGGGGCC‑GGCAACGACTTCCt > 6:252306/1‑101 (MQ=255) ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCt < 5:252306/101‑1 (MQ=255) ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGa > 6:394890/1‑149 (MQ=255) gcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGa > 2:154808/1‑148 (MQ=255) aacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGacac < 1:154808/149‑1 (MQ=255) cggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCa < 7:317247/149‑1 (MQ=255) gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGt > 1:152419/1‑110 (MQ=255) gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGt < 2:152419/110‑1 (MQ=255) acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAc > 7:144108/1‑149 (MQ=255) ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGgtggtg < 2:199391/115‑1 (MQ=255) ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGgtggtg > 1:199391/1‑115 (MQ=255) cTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCa < 7:335548/145‑1 (MQ=255) cTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCa > 8:335548/1‑145 (MQ=255) cGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGt > 2:359905/1‑149 (MQ=255) acaTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGGAACGACACCATGCTCGCCACCGATGGAAACGATGTGTGCCAGTTCGc > 8:86743/1‑149 (MQ=255) gACGTGGGTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTgg < 8:144108/149‑1 (MQ=255) gTACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGGGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAc < 7:86743/149‑1 (MQ=255) tACCGACACCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc > 5:217820/1‑149 (MQ=255) aCCGACACCCTGATCAGGGGGGCCGGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc > 1:201203/1‑149 (MQ=255) acacCCTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatc < 1:359905/149‑1 (MQ=255) ccTGATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc > 1:270240/1‑148 (MQ=255) gATCAGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg < 8:240585/119‑1 (MQ=255) | CTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCC‑GGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC > NC_002947/2921322‑2921600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |