| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 90.0% | 63.8 / ‑3.6 | 31 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (10/17); minor base A (3/0); total (13/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 96.7% | 71.0 / ‑4.3 | 31 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (12/17); minor base A (1/0); total (13/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑‑TCGGGCACG‑CCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCA > NC_002947/1499367‑1499639 || gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCATCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTcc > 1:74017/1‑149 (MQ=25)gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACCGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCCCAA‑CAGTCGCCGCGGTGCAAGGCCCCCGCGTTGCCGGTGT‑TC‑GCGGGCAcc‑cccccgcc > 5:289016/1‑143 (MQ=255) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TA‑GCGGGCACGCCCGCTCCca > 2:100375/1‑148 (MQ=25) gTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCcaca < 5:1435/149‑1 (MQ=21) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGAc < 1:308303/149‑1 (MQ=21) aaTTCATAGCCTTAACCAAGCCTTGGAACCACACCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAA‑AAGGCGCCGCGGTGCAGGGCACCGGCTTGGCCGGTTTGC‑‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGc < 2:11437/148‑1 (MQ=18) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCg > 8:89542/1‑149 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGc < 7:89542/148‑1 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCCCAA‑CACGCGCCCCCGTGCAATGCACCCGCGTTGGCGGTGT‑TA‑GCGGGCCCGCCCCCACCCCCAGACAGATTTCCCATTAACCGGGcta > 8:15027/1‑146 (MQ=255) aaGCCTTGCCACCACAGCTGGACTTCGGCTCCCAAACGGTGGGAGCGGGCGTCCCCGCGAAA‑CAGGCGCCGGGGTGCATGGCACGGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATc < 7:324099/149‑1 (MQ=21) ttGCCACCACCGCCGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGAc < 3:69026/120‑1 (MQ=14) gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGTCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACa < 1:100375/148‑1 (MQ=25) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCAGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGa > 7:204099/1‑149 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 7:359346/147‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGTCTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 1:91240/145‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt > 2:91240/1‑145 (MQ=21) cTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAA‑CCCGCGCCGCGGTGCCTGGCACCGGGGTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc < 5:367781/149‑1 (MQ=21) aaCGGTGGGAGCGGGCGGGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc < 3:353454/129‑1 (MQ=18) aCGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACGGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACa < 5:71002/120‑1 (MQ=17) gTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TA‑ACGGGCACGCCCGCGCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAAc > 7:347154/1‑148 (MQ=25) cccGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGAACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 2:312896/148‑1 (MQ=37) ccGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTAt > 6:214450/1‑94 (MQ=17) ccGCGAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTAt < 5:214450/94‑1 (MQ=17) cgcgAAA‑CAGGCGCCGCGGTGCCTGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttt > 7:246076/1‑115 (MQ=21) cgcgAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttt < 8:246076/115‑1 (MQ=21) gcgAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 4:66978/119‑1 (MQ=21) gcgAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt > 3:66978/1‑119 (MQ=21) cgAAA‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa < 7:15027/149‑1 (MQ=37) aa‑CAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 6:303859/1‑149 (MQ=37) gcgGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 7:150151/1‑143 (MQ=37) gcgGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑GCGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 8:150151/143‑1 (MQ=37) gtgttcgcGGGCACGCCCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa > 8:101755/8‑149 (MQ=255) || GCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAA‑CAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑‑TCGGGCACG‑CCGCTCCCACAGACTGA‑TTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCA > NC_002947/1499367‑1499639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |