Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,811 (T)7→6 intergenic (+48/+705) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8050T.97.4% 168.3 / ‑2.7 39intergenic (+42/+711)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (24/14);  total (24/15)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGC  >  NC_002947/4740658‑4740949
                                                                                                                                                   |                                                                                                                                                 
gCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCGAGGGCAGCCtt                                                                                                                                                  <  1:47682/149‑1 (MQ=255)
   cTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttt                                                                                                                                               <  3:289073/149‑1 (MQ=255)
      gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCtttttta                                                                                                                                             <  7:334545/148‑2 (MQ=255)
      gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttt                                                                                                                                               <  4:310096/146‑1 (MQ=255)
      gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttt                                                                                                                                               >  3:310096/1‑146 (MQ=255)
      gaaagaaaTGCACTCCAGCCAGTACAAGATCGGCCGTCCGCGCAAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGACCTCAGGCAACCattttaat                                                                                                                                            >  3:76771/1‑146 (MQ=255)
       aaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGACAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatt                                                                                                                                           >  8:132311/1‑146 (MQ=255)
         agaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttattt                                                                                                                                          <  3:348092/148‑5 (MQ=255)
           aaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc                                                                                                                                       <  8:129493/149‑8 (MQ=255)
            aaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc                                                                                                                                       <  8:135062/148‑8 (MQ=255)
                ctctCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc                                                                                                                                       >  1:150459/1‑137 (MQ=255)
                ctctCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc                                                                                                                                       <  2:150459/144‑8 (MQ=255)
                     cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGACAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                               <  7:132311/146‑1 (MQ=255)
                     cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                            >  3:146241/1‑149 (MQ=255)
                           gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                      <  4:146241/149‑1 (MQ=255)
                           gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                      >  8:21417/1‑149 (MQ=255)
                            tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcg                                                                                                                     >  3:34518/1‑149 (MQ=255)
                               aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTCCGGCCTATTAGCgg                                                                                                                  >  8:266204/1‑149 (MQ=255)
                               aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                  >  1:33110/1‑149 (MQ=255)
                               aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGCGCCGGCCTCTTCGCgg                                                                                                                  <  4:239918/149‑1 (MQ=255)
                                   tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                              <  7:354392/149‑1 (MQ=255)
                                   tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                              <  5:268798/149‑1 (MQ=255)
                                         gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                              <  4:187665/127‑1 (MQ=255)
                                         gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                              >  3:187665/1‑127 (MQ=255)
                                          tCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcg                                                                                                                     <  3:242238/135‑1 (MQ=255)
                                          tCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcg                                                                                                                     >  4:242238/1‑135 (MQ=255)
                                                                   cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaa                                                                               >  1:4695/1‑148 (MQ=255)
                                                                     gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACCAAAc                                                                            >  7:301840/1‑149 (MQ=255)
                                                                      aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                     <  6:177125/123‑1 (MQ=255)
                                                                      aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                     >  5:177125/1‑123 (MQ=255)
                                                                           cgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGt                                                                      >  1:233243/1‑149 (MQ=255)
                                                                                       ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                          <  2:233243/149‑1 (MQ=255)
                                                                                         tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccgc                                                        >  6:199640/1‑149 (MQ=255)
                                                                                         tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccgc                                                        >  2:214069/1‑149 (MQ=255)
                                                                                         tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCATAATCGCGGCCATGGCCCGGAAGAGGCCGGAACAGACAACACAAAACCTAAGTTTTTATcagcccc                                                         >  7:53636/1‑147 (MQ=255)
                                                                                              taCCGAAAGTAAGCTGCAGTAGCCTAACGAAAGAAGGCTGCCCTCGGGCTG‑CTTTTTTTATTTGTCCTGTGCCGGCCTATTCGCGGCTTTGCCCGGTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑Cg                                                  <  2:4695/148‑1 (MQ=255)
                                                                                                   cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTCATACCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGATTTTATCCGCCCCCTCA‑CGCAccc                                             >  3:262538/1‑149 (MQ=255)
                                                                                                              cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTa                                  <  1:214069/149‑1 (MQ=255)
                                                                                                                 tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTAtt                                >  1:88495/1‑148 (MQ=255)
                                                                                                                    ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGAATACCGGGCGTGGCCCCCCAGAGGCCGGCCCCCACACCCCAAAAACGCACGTTTTGTACCCCCCCACACCCCACCCCTTGAAGGGTAttaa                              >  4:186369/1‑146 (MQ=255)
                                                                                                                        aCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTGTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGgcg                        >  1:231961/1‑149 (MQ=255)
                                                                                                                        aCGCAACCAGGCGGCCCGCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGgcg                        <  8:301840/149‑1 (MQ=255)
                                                                                                                         ataaaaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCGTTCAGGGTATTGAGCGgcgc                       >  6:374020/7‑149 (MQ=255)
                                                                                                                          taaaaaagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCa                      >  5:101565/6‑149 (MQ=255)
                                                                                                                                agGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                 >  3:187563/1‑148 (MQ=255)
                                                                                                                                 ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca               >  5:158922/1‑149 (MQ=255)
                                                                                                                                     gCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                            <  7:86844/85‑1 (MQ=37)
                                                                                                                                     gCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                            >  8:86844/1‑85 (MQ=37)
                                                                                                                                         tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGCAGAGGCCGGGCCACACCCCCCAAAACCTCAGTTTTTATACGCCCCCCCA‑CGCCCCCCGTTGAGGGTATTGAACGGCGCATCCACCACCAACttt       >  6:19833/1‑148 (MQ=255)
                                                                                                                                            ggCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg    <  6:101565/149‑1 (MQ=255)
                                                                                                                                              cAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGc  <  2:33110/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGC  >  NC_002947/4740658‑4740949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑