| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 97.7% | 145.7 / ‑3.1 | 44 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (25/18); total (26/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT > NC_002947/1932494‑1932780 | cggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATGGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCACTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑cccg > 1:357547/1‑147 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCCACCCCCCTACTAACAAGGATAATCGTCCCCCATATGAGACGGC‑GTGCCCGCGAAggg > 6:330270/1‑149 (MQ=255) gATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGAATTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGAAAGCCGGCTCCTAAAAAGGATTTGCGTCCCGTGTAGGTGGGGGG‑TTGCCCGCGAAGGGCTGCAAGGc < 8:38456/149‑1 (MQ=255) aTCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAgg > 1:300907/1‑147 (MQ=255) gACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGt > 6:172934/1‑149 (MQ=255) aGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGa < 7:62752/149‑1 (MQ=255) ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCCCTCCAACAAAGGATATGCGTACCCTGTAGGAGCCGGCTTTC‑CCGCGAAGGGCTGCAAGGCAGCCCCAAAATGTCGAcaa > 2:328111/1‑147 (MQ=37) gCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCgg > 7:220938/1‑148 (MQ=255) ggctcACGAAAATCGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTGCGC‑GGGCAAGCCCGCTCCTACAACGCATATGCGTCCCCTGTCGGAGCGGGG‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 4:130986/132‑1 (MQ=25) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc > 3:130986/1‑134 (MQ=255) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGa > 1:268302/1‑149 (MQ=255) ccGAAAAAAGCCTTAACCGCTGGGGCCGCTTTGCGGCCCTGCGC‑GGGCAAGCCCGCTCCTACAAAGCATATGCGTCCCCTGTCGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGa < 5:330270/146‑1 (MQ=38) aCGAAAATCGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCgg < 2:286501/139‑1 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCgg > 1:286501/1‑140 (MQ=255) aCGAAAATACCAGTAAACGCGGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATACCCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 7:100004/129‑1 (MQ=37) gCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑Tc > 6:358732/1‑146 (MQ=255) cGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGaa < 2:93658/149‑1 (MQ=255) gCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCATGTAGGAGCGGGA‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCAGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAAt > 6:182329/1‑149 (MQ=38) cTTTGCGGCCTTTCGC‑GGGCAAGCCCGCTCATACAAAGTATATGCGTCCCTTTTTGGTGGGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATc < 2:300907/149‑1 (MQ=255) agCCCTTCGC‑GGGCAAGCCCGCTCCTACAGGGGACGCATATCCTTTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGagcag > 6:182475/2‑149 (MQ=18) gggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGTGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGa > 2:331251/1‑135 (MQ=255) gggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGTGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGa < 1:331251/135‑1 (MQ=255) gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTc > 6:301536/1‑147 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAAtt < 6:380983/75‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAAtt > 5:380983/1‑75 (MQ=255) cccACTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg < 2:357547/149‑1 (MQ=255) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCg < 1:349484/126‑1 (MQ=255) cGCTCCTACAATGGATATGGGTCCCCTGTAGTTGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt < 1:328111/149‑1 (MQ=255) tCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGTAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa > 1:278721/1‑149 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 7:306967/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 4:391636/1‑146 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat < 3:391636/146‑1 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 1:6673/1‑149 (MQ=255) atGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc > 3:317895/1‑149 (MQ=255) tGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 5:358732/148‑1 (MQ=255) cGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt > 3:221983/1‑149 (MQ=255) tcctttGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 5:182475/144‑1 (MQ=255) tCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 5:172934/149‑1 (MQ=255) cccTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc > 8:254284/1‑148 (MQ=255) ggAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca > 5:343417/1‑149 (MQ=255) ggAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca > 4:296149/1‑149 (MQ=255) cGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACata > 3:262505/1‑149 (MQ=255) cGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACata < 2:268302/149‑1 (MQ=255) gggC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAATGTAGGAACGAACAGAGAGGAGCAGCAGCCCTGGTTTTACAGCTCGATATTCACATCATAGTTGCAGATGTTcaca > 1:321568/1‑149 (MQ=255) ggC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAtt > 2:115446/1‑149 (MQ=255) | CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGC‑GGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTG‑ATGT‑TCAGC‑TCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT > NC_002947/1932494‑1932780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |