| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 3,845,705 | +G | intergenic (‑72/+26) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 95.9% | 156.9 / 0.2 | 49 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (27/20); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01 | |||||||||||
GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAG > NC_002947/3845556‑3845841 | ggCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGGCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTcg‑c < 8:91107/148‑1 (MQ=255) ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑ggg < 2:240611/149‑2 (MQ=255) ctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGG‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑gggc < 6:84415/147‑3 (MQ=255) gTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGAt > 4:329477/1‑149 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGAt < 4:232181/148‑1 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGAt > 7:72840/1‑148 (MQ=255) cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATc < 8:2539/149‑1 (MQ=255) cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATc < 7:95645/149‑1 (MQ=255) gatggcTTGCAGAGCCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGt > 3:345636/6‑148 (MQ=255) gATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGt > 6:305422/1‑148 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTAAGTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGt > 1:98333/1‑147 (MQ=255) tttgaGGCTCTGCAAGCCATCGCGCAGGGCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAACCGTTTCAGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCa < 1:118907/145‑1 (MQ=255) gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑TTGATCCATCGTCAGCCa < 5:108316/146‑1 (MQ=255) gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑TTGATCCATCGTCAGCCa > 6:108316/1‑146 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGa < 3:329477/148‑1 (MQ=255) gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGa < 1:7469/149‑1 (MQ=255) ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGAt < 6:356142/149‑1 (MQ=255) ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGAt > 1:261659/1‑149 (MQ=255) cATGGCGAAGGCCGACTTCATTTATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTTAAGCGTTGC‑GCCAGATAAATAGCTGTATGACGGGCATAAAAGACGTGTTTCT‑C‑GGTCTGATCCATCGTCAGCCACGATGGATCTGCCCgcgc < 2:98333/149‑1 (MQ=255) cATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCgcgc > 2:103729/1‑149 (MQ=255) aCTTCTTTCATTGTT‑ATTGTTGTCCCCGCGGCCTGTCTGTCTCACGGGTGGC‑GCCTCACAAATAGCTGGTTGCCGGCCATAAACGCGGTGTCGCG‑CGGG‑CTCACCCATCGTCCCCCAGTATGCATCAGCCCGCGCCCTGCCAGGCCGGt < 6:89704/149‑1 (MQ=255) cTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCC‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTg < 1:103729/149‑1 (MQ=255) ttCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTg > 8:355736/1‑148 (MQ=255) ttgtt‑attgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGcc > 8:34593/1‑106 (MQ=255) ttgtt‑attgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGcc < 7:34593/106‑1 (MQ=255) ttgttgTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggt > 2:244597/1‑149 (MQ=255) aTCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCCGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGc > 3:99689/1‑149 (MQ=255) aTCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc < 3:44262/145‑1 (MQ=255) aTCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc > 4:44262/1‑145 (MQ=255) tCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAg > 1:65971/1‑147 (MQ=255) tCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATACATCGTCAGCCACGATGGATAAGCCCGCGCCCTGCCACGCCGGTGCACGCACCAACAGGTGGTCAAg > 2:216426/1‑147 (MQ=255) gcgGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGAt < 4:34818/94‑1 (MQ=255) gcgGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGAt > 3:34818/1‑94 (MQ=255) gcgctgtgtctgAAGGGTTGC‑GCCAGACTAAGAGCTGGATTCCGGGCAGAAAAGCCGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGAGGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc < 6:185297/143‑1 (MQ=255) tctgtctgAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc > 5:185297/1‑145 (MQ=255) tgAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAg > 6:360535/1‑87 (MQ=255) tgAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAg < 5:360535/87‑1 (MQ=255) aaGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGt < 6:105151/68‑1 (MQ=255) aaGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGt > 5:105151/1‑68 (MQ=255) cGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCATAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCgaga < 2:261659/149‑1 (MQ=255) ttGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGc < 1:244597/149‑1 (MQ=255) agCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtg < 8:95433/103‑1 (MQ=255) agCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtg > 7:95433/1‑103 (MQ=255) ggATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGACCCCACAGAGAGACCGTGTGCCCGTCGTGCATg > 7:298844/1‑148 (MQ=255) gATGACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg > 6:93873/1‑147 (MQ=255) gACGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGCCAGCGAGAGCGTGTGCCGGTcgc > 5:236385/1‑139 (MQ=255) cGGGCAGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGc > 7:229538/1‑130 (MQ=255) aGAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAt < 5:93873/149‑1 (MQ=255) gAAAAGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAtt < 2:65971/149‑1 (MQ=255) aaaaGACGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGc > 1:134821/1‑136 (MQ=255) gACGTGTTTCG‑CGGG‑CTGATCCATCGTAAGCCAAGATGGATAAGCCCGCGCCCTACCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCAcc > 8:344280/1‑148 (MQ=255) cGTGTTTCG‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAg > 7:276905/1‑149 (MQ=255) | GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTT‑ATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGC‑GCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAG > NC_002947/3845556‑3845841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |