| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 307,866 | +C | intergenic (+881/‑988) | hslO → / → PP_0254 | molecular chaperone HslO/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 307,865 | 1 | . | C | 98.7% | 246.8 / ‑4.4 | 75 | intergenic (+880/‑989) | hslO/PP_0254 | molecular chaperone HslO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (31/42); minor base A (1/0); total (33/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01 | |||||||||||
AAAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑C‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAG > NC_002947/307717‑308004 | aaaGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAg < 3:371925/149‑1 (MQ=255) cATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgtt < 2:68248/149‑5 (MQ=255) aTGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgtta > 4:119134/1‑144 (MQ=255) gTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTTTGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑cc > 2:85617/1‑149 (MQ=255) gTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGTCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑c < 8:66871/148‑1 (MQ=255) ttCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGTCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCt < 8:276943/149‑1 (MQ=255) ttCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCt < 4:24191/149‑1 (MQ=255) ctcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGa > 6:258840/1‑149 (MQ=255) cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTga > 4:332499/1‑149 (MQ=255) cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTg < 6:37689/148‑1 (MQ=255) aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTgac < 6:171844/149‑1 (MQ=255) aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTgac > 7:244078/1‑149 (MQ=255) gAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTgacg < 2:363365/149‑1 (MQ=255) cTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTgacgac > 8:320165/1‑148 (MQ=255) cTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGacgacg > 4:53874/1‑149 (MQ=255) ctgctgCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCa < 4:210566/149‑1 (MQ=255) ctgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACgg > 6:78912/1‑149 (MQ=255) tgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGt > 2:78116/1‑149 (MQ=255) tgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGt > 6:341336/1‑149 (MQ=255) ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCCAGCCTACCCTTGAGGCGGACGGACGCC‑GGTA‑CCTCATCGGTGACGCCGCGGCCGGTTggg > 4:291529/1‑149 (MQ=255) ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTggg < 5:258840/149‑1 (MQ=255) ccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCTGCACTGGCAATACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGaa < 4:330861/149‑1 (MQ=255) ccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCg > 8:247568/1‑147 (MQ=255) tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt < 1:109648/149‑1 (MQ=255) tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGt < 7:247568/148‑1 (MQ=255) aCTGTCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCCTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt < 2:353165/148‑1 (MQ=255) gCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt > 4:15624/1‑149 (MQ=255) ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACTAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt < 1:285857/149‑1 (MQ=255) ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc < 1:78116/147‑1 (MQ=255) ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt < 8:244078/149‑1 (MQ=255) ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt < 3:15624/148‑1 (MQ=255) cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc < 3:332499/149‑1 (MQ=255) tATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGAAAGACTACCATTTAGGCTGACGAAAGAC‑GTTA‑CCTGATCGGTGACGACGATCGCGGTTGGGGCGATGGCGtt > 6:399880/1‑142 (MQ=255) tATCCACTGCGCGGCCAACATCGGCTAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCCAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGAAGACGAGCACGGTTGGTGCGAAGGCGtt < 5:399880/142‑1 (MQ=255) tGCACTGGGCGGCCAACATGGGCGAAGCAGGCGATGGGACCGTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTTCCCTTTCGGCAGACGAAAGCC‑GTTACCCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa < 1:286432/149‑1 (MQ=255) tGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CAGTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATgg > 2:58074/1‑148 (MQ=255) gcgcGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc > 1:59801/1‑140 (MQ=255) gcgcGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc < 2:59801/140‑1 (MQ=255) cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGTCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAg < 2:350925/149‑1 (MQ=255) cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa < 5:78912/148‑1 (MQ=255) cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa < 3:119134/148‑1 (MQ=255) cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa < 3:53874/148‑1 (MQ=255) cgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggc > 8:129290/1‑149 (MQ=255) gCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCATCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACTGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcgg < 7:362358/149‑1 (MQ=255) cGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt < 7:29735/118‑1 (MQ=255) cGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt > 8:29735/1‑118 (MQ=255) gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAATGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa < 1:58074/149‑1 (MQ=255) aagaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAg < 1:85617/149‑1 (MQ=255) agaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGTCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt < 7:320165/149‑1 (MQ=255) aagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt < 5:116295/113‑1 (MQ=255) aagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt > 6:116295/1‑113 (MQ=255) tgtgACCTTGTTCTTCGGCCTGTCAGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAcc > 7:327799/1‑149 (MQ=255) aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc < 1:228696/144‑1 (MQ=255) aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc > 2:228696/1‑144 (MQ=255) aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCATTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGt < 5:341336/148‑1 (MQ=255) cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc > 5:361783/1‑148 (MQ=255) cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc > 3:211766/1‑148 (MQ=255) cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCg < 8:33233/149‑1 (MQ=255) tcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa > 7:253772/1‑110 (MQ=255) tcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa < 8:253772/110‑1 (MQ=255) cttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag < 7:129290/148‑1 (MQ=255) ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGTCTGCTACGCCAAGTGCATCGAACTGTCCGAgaagaa > 3:215289/1‑149 (MQ=255) ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa > 3:49320/1‑149 (MQ=255) cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTAc > 8:364449/1‑114 (MQ=255) cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTAc < 7:364449/114‑1 (MQ=255) cGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCa < 4:211766/149‑1 (MQ=255) ggCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCAt > 3:325433/1‑149 (MQ=255) tGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg > 7:277946/1‑139 (MQ=255) tGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg < 8:277946/139‑1 (MQ=255) cAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGGTGTCTTCAACATGGAAGGCGGCTGCGACGCCAAGGGCCTCGACCTGTCCGAGAAGAACGAGGCGGGCATCTGGaacg > 4:213749/1‑147 (MQ=255) aCCCTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc > 4:284372/1‑147 (MQ=255) ccTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGAATCGACCTGTCCGAGAAGAACGAGc > 6:376096/1‑125 (MQ=255) ccTTTCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGAATCGACCTGTCCGAGAAGAACGAGc < 5:376096/125‑1 (MQ=255) tttCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc < 4:118171/143‑1 (MQ=255) tttCGGCTGACGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc > 3:118171/1‑143 (MQ=255) aCGAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCTGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg < 3:291529/147‑1 (MQ=255) gAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCa < 4:279822/144‑1 (MQ=255) gAAAGCC‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCa > 3:279822/1‑144 (MQ=255) | AAAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑C‑GTTA‑CCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAG > NC_002947/307717‑308004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |