| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 5,681,415 | +CGGG | intergenic (‑133/+71) | PP_4986 ← / ← PP_4987 | hemolysin III family channel protein/putative Chemotaxis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,681,415 | 1 | . | C | 92.9% | 28.6 / ‑2.5 | 14 | intergenic (‑133/+71) | PP_4986/PP_4987 | hemolysin III family channel protein/putative Chemotaxis protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (8/5); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 | |||||||||||
| * | NC_002947 | 5,681,415 | 2 | . | G | 92.9% | 32.5 / ‑2.5 | 14 | intergenic (‑133/+71) | PP_4986/PP_4987 | hemolysin III family channel protein/putative Chemotaxis protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (8/5); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
| * | NC_002947 | 5,681,415 | 3 | . | G | 92.9% | 32.9 / ‑2.5 | 14 | intergenic (‑133/+71) | PP_4986/PP_4987 | hemolysin III family channel protein/putative Chemotaxis protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (8/5); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 5,681,415 | 4 | . | G | 92.9% | 32.9 / ‑2.5 | 14 | intergenic (‑133/+71) | PP_4986/PP_4987 | hemolysin III family channel protein/putative Chemotaxis protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (8/5); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTC > NC_002947/5681272‑5681545 |||| ccGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACc‑‑‑‑gccgggg < 2:30137/149‑6 (MQ=255) atgTGGGATGGGTTCGTGTTGTGATGCTGTTGGCTTCGTTCTTTGTGGGAGCGGCGTTGTGTCGCGAAAGGGGCGCGAAGCGGGCGCAGGCTTTCATTGCCTCTGCATTAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAgg < 8:121221/147‑1 (MQ=21) aGGTGGACTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTTTCGCGAAAGGGGCGCGTAGCGGCCCCATGCTTTCATTGGCACTTGATTAACC‑‑‑‑GCCGGGGCTGGTTCGCAGCCCTATAGCGACACaatg > 3:38646/1‑147 (MQ=21) gggTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTTCCACTGCCTGAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCGATAGCGACACAAGGCCGCAcccc > 6:86469/1‑148 (MQ=21) tGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTGCCACTCCAGACCACCAGGccc > 1:38973/1‑149 (MQ=25) ttgtttGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCt < 2:136195/113‑1 (MQ=18) ccgctAATGGGGCGCTACGGGGCCCCTGGTTTTAATTCCCACTTCATTAACCCCGGGG‑‑‑‑GCTGCTGCCCAGCCATATCGCAAAAAATGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCa < 7:244304/147‑1 (MQ=255) cgAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GCCGGGGCTGCTGCGCCGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATc > 5:365185/1‑149 (MQ=38) atggggCGCGAAGCGGCCCAAGGCTTTCATTGCCACTGCATTAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCTACAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCcgc < 2:38973/147‑1 (MQ=255) gggggCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGaccacc < 5:289580/101‑1 (MQ=25) gggggCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGaccacc > 6:289580/2‑102 (MQ=25) gcgAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACt < 5:200038/86‑1 (MQ=21) gcgAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACt > 6:200038/1‑86 (MQ=21) cccAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GCCGGGGCTGCTGGGCAGCCCTATCGCGGCACAAAGCCGCTCCCCCGCCAGACCACCCGGCCCTGCGCCGCACCTCCTAACCCAGGTCAGGGTGCCGATCCGCGGCACGCCCCAGCCgtt > 7:257100/1‑149 (MQ=255) cACTGCATGAACC‑‑‑‑GCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGCCCACCCGGCCCTGCGCCGCACATCCTAACCAACGTCAGGTTGCAGATACGCGTCAGGCACCAGCCGTTCCAATGCGACCAGGTc > 5:238905/1‑149 (MQ=37) |||| CCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACC‑‑‑‑GC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTC > NC_002947/5681272‑5681545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |