Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,070,248 +GA intergenic (+48/+94) aroP‑I → / ← PP_0928 aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,070,2461.G90.6% 261.9 / 21.0 86intergenic (+46/+96)aroP‑I/PP_0928aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
Reads supporting (aligned to +/- strand):  ref base . (4/4);  new base G (41/36);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0029471,070,2462.A90.7% 262.6 / 20.1 86intergenic (+46/+96)aroP‑I/PP_0928aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
Reads supporting (aligned to +/- strand):  ref base . (4/4);  new base A (42/36);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑T‑‑G‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTAC  >  NC_002947/1070098‑1070378
                                                                                                                                                      ||                                                                                                                                         
ccTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtg‑t                                                                                                                                             <  3:229365/149‑1 (MQ=255)
 cTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtg‑t                                                                                                                                             >  7:40360/1‑148 (MQ=255)
    aCGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑T‑‑G‑‑a                                                                                                                                       <  8:50234/147‑1 (MQ=255)
      gccgccGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtg‑t                                                                                                                                             >  1:117380/1‑143 (MQ=255)
      gccgccGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtg‑t                                                                                                                                             >  8:18277/1‑143 (MQ=255)
      gccgccGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCtg‑t                                                                                                                                             >  7:287809/1‑143 (MQ=255)
       ccgccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑T‑‑G‑‑agatc                                                                                                                                   >  4:55755/1‑144 (MQ=255)
        cgccgATTCGCATCTCGGTAATGCTGATCCCTGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑T‑‑G‑‑agatcga                                                                                                                                 <  7:73147/149‑7 (MQ=255)
         gccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑T‑‑G‑‑agatcgag                                                                                                                                >  7:207799/1‑142 (MQ=255)
          ccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑T‑‑G‑‑agatc                                                                                                                                   >  7:246128/1‑141 (MQ=255)
            gATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑Agcgc                                                                                                                            >  7:62424/1‑149 (MQ=255)
             aTTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGcgcc                                                                                                                           >  7:327548/1‑149 (MQ=255)
                  cATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCcgcgcg                                                                                                                      >  2:75401/1‑149 (MQ=255)
                   aTCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCgcgg                                                                                                                     >  1:226890/1‑149 (MQ=255)
                     ctcGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCgcgggc                                                                                                                   >  5:116658/1‑149 (MQ=255)
                       cGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCgcgggcgg                                                                                                                 >  7:277908/1‑149 (MQ=255)
                        ggTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGggcggc                                                                                                                <  4:325380/149‑1 (MQ=255)
                          tAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCTTTAGGCTTTCATCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCCCGCGCGgcgc                                                                                                              >  5:237398/1‑149 (MQ=37)
                            aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCTTTAGGCTTTCATCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCCCGCGCGGCGCt                                                                                                             <  6:237398/148‑1 (MQ=37)
                            aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCt                                                                                                             <  3:55755/148‑1 (MQ=255)
                            aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTc                                                                                                            <  7:18277/149‑1 (MQ=255)
                             tGCTGATCCCGGCATGGATCGCTGTGCTGTTGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCCCGCGCGGCGCTCg                                                                                                           <  8:272317/149‑1 (MQ=38)
                             tGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCATCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCCCGCGCGGCGCTCg                                                                                                           <  1:14716/149‑1 (MQ=37)
                               cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGAt                                                                                                         <  6:12496/149‑1 (MQ=255)
                               cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGCTTTCATCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCCCGCGCGGCGCTCGAt                                                                                                         <  8:255319/149‑1 (MQ=37)
                                tGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGAGCTCGAtc                                                                                                        >  6:180958/1‑149 (MQ=255)
                                 gATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGAtct                                                                                                       >  1:248359/1‑149 (MQ=255)
                                   tCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                     <  8:277908/149‑1 (MQ=255)
                                     ccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                     >  4:22813/1‑147 (MQ=255)
                                     ccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGATCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGTCGCTCGATCTca                                                                                                     >  4:28092/1‑147 (MQ=39)
                                      cgatcgtgATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCTGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCTCTCGATCTCACAg                                                                                                  <  3:250301/142‑1 (MQ=38)
                                             gATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCAGCGCGGGCGGCGCTCGATCTcacat                                                                                                  >  4:250301/1‑141 (MQ=255)
                                                  ctgtgctgtgGGTGGCTTTCAATCTGAAGAAGGATCGCCAGACCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑T‑‑GATA‑‑TAG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGcc                                                                                      >  7:248344/1‑149 (MQ=38)
                                                  ctgtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑CGCGCCGCGCGGGCGGGGGTGGCTATCCCAGGCGATGAAAGcc                                                                                      >  4:5041/1‑149 (MQ=255)
                                                  ctgtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGcc                                                                                      >  8:369716/1‑149 (MQ=255)
                                                   tgtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCCAAGGCGATGAAAGcc                                                                                      >  1:51625/1‑148 (MQ=38)
                                                       ctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAg                                                                                 <  6:124388/149‑1 (MQ=255)
                                                            ggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATg                                                                            <  8:207799/149‑1 (MQ=255)
                                                                gCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTc                                                                        <  8:40360/149‑1 (MQ=255)
                                                                  tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑ATCGACCCGCGGGAGGCGCACGAGCTCAAAgaggat                                                                                             >  2:42707/1‑121 (MQ=255)
                                                                  tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGaaa                                                                                         >  8:339544/1‑130 (MQ=255)
                                                                  tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGaaa                                                                                         <  7:339544/130‑1 (MQ=255)
                                                                  tttCAAGCTGAAGAAGGCTCGACAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGTGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCTTCGCGCGGGCGGCGCTCGATCTCACAGGCGAt                                                                                             <  1:42707/126‑1 (MQ=37)
                                                                    tCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggc                                                                    >  2:46082/1‑149 (MQ=255)
                                                                      aaGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggcgg                                                                  <  2:248359/149‑1 (MQ=255)
                                                                      aaGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCAGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggcgg                                                                  >  4:236971/1‑149 (MQ=255)
                                                                      aaGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TAG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggcgg                                                                  >  2:192897/1‑149 (MQ=255)
                                                                        gCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCGGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                                <  2:117380/149‑1 (MQ=255)
                                                                        gCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                                >  1:252433/1‑149 (MQ=255)
                                                                        gCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                                <  6:116658/149‑1 (MQ=255)
                                                                        gCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCATCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCCCGCGCGGCGCt                                                                                                             <  2:351669/104‑1 (MQ=21)
                                                                        gCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCATCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCCCGCGCGGCGCt                                                                                                             >  1:351669/1‑104 (MQ=21)
                                                                          tGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCt                                                              >  3:225126/1‑149 (MQ=255)
                                                                            aagaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCCGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGa                                                            <  3:236971/149‑1 (MQ=255)
                                                                              gaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTAGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGACGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAcg                                                          >  4:274683/1‑149 (MQ=38)
                                                                               aagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCgcgggcg                                                                                                                  <  3:224767/92‑1 (MQ=37)
                                                                               aagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCgcgggcg                                                                                                                  >  4:224767/1‑92 (MQ=37)
                                                                                  gCTCTCCAGTCCAAGGTCTTTTCGTTTTTTTTCGTATGGGTTTTGCCGTTTGGTTTTCGGGGGTTGTT‑‑T‑‑T‑‑TTGTAGCGCGGTTTGGGTGGCGTTTGATCTCACAGGTGATGAAAGTCTAAAGGCATGCGTCTGGCGGCCCTGACGCGtt                                                      <  8:248344/149‑1 (MQ=255)
                                                                                  gCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGtt                                                      >  7:276751/1‑149 (MQ=255)
                                                                                   cTCGCCAGGCCAAGGGCTGATCGGGTTTGTTGGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTc                                                     <  8:327548/149‑1 (MQ=255)
                                                                                          ggCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCTCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                              <  3:54779/149‑1 (MQ=255)
                                                                                          ggCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                              <  3:28092/149‑1 (MQ=255)
                                                                                          ggCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc                                              <  3:22813/149‑1 (MQ=255)
                                                                                           gCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGaaa                                                                                         >  3:314064/1‑105 (MQ=255)
                                                                                           gCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGaaa                                                                                         <  4:314064/105‑1 (MQ=255)
                                                                                             cAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATg                                           >  7:307493/1‑149 (MQ=255)
                                                                                               aGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                                >  8:226780/1‑126 (MQ=255)
                                                                                               aGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                                <  7:226780/126‑1 (MQ=255)
                                                                                                gggCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGccc                                        >  2:232137/1‑149 (MQ=255)
                                                                                                       gcGGGTTTGTTGGTAAGGGGTCTGCCGTTAGGTGTGCGGCGCCTG‑G‑‑G‑‑AGCGCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtg                                 <  8:287809/148‑1 (MQ=38)
                                                                                                          ggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTAACCTTCCATGCCCCTCAAagctg                              >  4:126934/1‑149 (MQ=38)
                                                                                                           gTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCt                                                                       >  6:108531/1‑107 (MQ=255)
                                                                                                           gTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCt                                                                       <  5:108531/107‑1 (MQ=255)
                                                                                                           gTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgctgc                             <  8:276751/149‑1 (MQ=255)
                                                                                                             ttGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCt                           >  8:239294/1‑149 (MQ=255)
                                                                                                                 tCGTAAGGGGTATGCCGTTAGGTTTTCGGCCCCTG‑TTAG‑‑A‑‑TAC‑AGCGCCGCGCGGGCGGCGCGAGATCACACAGCCGATTAAAGCCTAAAGGCAAGCCTCAGGCCGCCCAGACCCGGTACCCATCCATGCCCCACAAAGAAGCCACCGc                       >  4:192736/1‑149 (MQ=255)
                                                                                                                   gTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGcc                      <  7:369716/148‑1 (MQ=255)
                                                                                                                   gTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGcc                      <  8:307493/148‑1 (MQ=255)
                                                                                                                   gTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCAAGCCCCTCAATGCTGCCTCCGcc                      >  6:281798/1‑148 (MQ=255)
                                                                                                                     aaGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTGGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGGTACCCTTACATGCCCCTCAAAGCTGGCTCCCCCAAg                   >  6:32095/1‑149 (MQ=37)
                                                                                                                       ggggTATGCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgca                 <  2:252433/149‑1 (MQ=255)
                                                                                                                              gCCGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAg          >  1:100479/1‑149 (MQ=255)
                                                                                                                                cGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg        <  8:62424/149‑1 (MQ=255)
                                                                                                                                cGTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCCGCGCTGGATCTAACAGGCGATTAAACCCTAAAGGCAAGCGTCTGGCGGCCCTGCCGCGTTCCGCTTCCAAGCCCCTCAAAGCTGCCTCCGCCAATCACGCACAgtg        >  2:246944/1‑149 (MQ=25)
                                                                                                                                 gTTAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtgg       <  1:232137/149‑1 (MQ=255)
                                                                                                                                  ttAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggt      >  6:306068/1‑149 (MQ=255)
                                                                                                                                  ttAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggt      >  4:266434/1‑149 (MQ=255)
                                                                                                                                  ttAGGTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggt      <  3:274683/149‑1 (MQ=255)
                                                                                                                                      gTTTTCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTAc  >  7:361441/1‑149 (MQ=255)
                                                                                                                                       ttttCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAt                                  <  4:320387/116‑1 (MQ=255)
                                                                                                                                       ttttCGGCGCCTG‑TGAG‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAt                                  >  3:320387/1‑116 (MQ=255)
                                                                                                                                                      ||                                                                                                                                         
CCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTG‑T‑‑G‑‑A‑‑TCG‑AGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTAC  >  NC_002947/1070098‑1070378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑