Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,921,460	G→A	G3494R (GGG→AGG)	PP_2561 →	hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,921,460	0	G	A	97.5%	132.9 / NA	40	G3494R (GGG→AGG)	PP_2561	hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/16);  minor base T (0/1);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATTT  >  NC_002947/2921314‑2921605                                                                                                                                                   |                                                                                                                                                  acacGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACTGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagg                                                                                                                                                 >  7:320493/1‑149 (MQ=255)     gTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagggggg                                                                                                                                             >  4:130424/1‑149 (MQ=255)            ggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCaa                                                                                                                                      <  3:227671/149‑1 (MQ=255)                 ggCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACt                                                                                                                                 <  3:130424/149‑1 (MQ=255)                           gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg                                                                                                                       >  6:168522/1‑149 (MQ=255)                           gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATCACACCCTGCAGGTCGGCGATGGTAACGAAACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg                                                                                                                       >  6:229803/1‑149 (MQ=255)                                ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                  >  3:267102/1‑149 (MQ=255)                                   gAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAg                                                                                                               >  3:120070/1‑149 (MQ=255)                                                       aCAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggt                                                                                           <  5:168522/149‑1 (MQ=255)                                                        cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGgtggtg                                                                                              >  1:106205/1‑145 (MQ=255)                                                        cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGgtggtg                                                                                              <  2:106205/145‑1 (MQ=255)                                                         aGCCTGGATGGCGGCAACGGCAATGACACACTGCAGGGCGGAGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCAGGCAACGACTTCCTAAACGGAGCGGCAGGTAACGAGACGGAGgagggtgg                                                                                         >  6:287275/1‑149 (MQ=255)                                                            atgtaTGGCGTCAACGGCAATGACACCATGCATGGAGGCTATGGTATCTAAACATTGTTCGGCGACGTGGGTACCGACACCGTGATCTGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGc                                                                                      <  8:320493/145‑1 (MQ=255)                                                               gATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTgggg                                                                                       <  5:175917/145‑1 (MQ=255)                                                               gATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTgggg                                                                                       >  6:175917/1‑145 (MQ=255)                                                                         acggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACAcc                                                                         <  4:120070/149‑1 (MQ=255)                                                                           ggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                       >  8:329238/1‑149 (MQ=255)                                                                            gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                       >  3:337532/1‑148 (MQ=255)                                                                               aTGACAAACTTCAGGGCGGCGATGGTAACGACACATTTTTCTTCGACGTGGCTACCGACACCCTTATCAGGGGGGCCGTCAACGACTTCCTCAACGGTGCTGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATc                                                                   <  2:19237/149‑1 (MQ=255)                                                                                   cacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCa                                                               >  7:291082/1‑149 (MQ=255)                                                                                    acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAc                                                              >  6:179339/1‑149 (MQ=255)                                                                                    acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAc                                                              >  8:162800/1‑149 (MQ=255)                                                                                          cAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt                                                          <  4:267102/147‑1 (MQ=255)                                                                                           aGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGAAGGTAACGATACGGTGGTGGGTGGGGCAGGTAACGACCACATGATCGACACCGATgg                                                        >  3:73789/1‑148 (MQ=255)                                                                                                 gcgATGGTAACGACACATTGTTCGGCGACGTGGGGACCGACACCGTTAGCAGGGGGGCCGGCAACTACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGACCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                                 <  5:229803/149‑1 (MQ=255)                                                                                                          aCGACACATTGTTCGGCGACGTGGGTACAGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                           >  5:144792/1‑146 (MQ=255)                                                                                                          aCGACACATTGTTCGGCGACGTGGGTACAGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCa                                         <  6:144792/148‑1 (MQ=255)                                                                                                               acaTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCATCAACGGTGCGGAAGGTAACGATACGTTGGTGGGTGGGGACGGTAACGACACCATGATCGCCAACGATGGAAACTATGTGTTCCAGTTCGc                                   >  4:73465/1‑149 (MQ=255)                                                                                                                     ttCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAgg                             >  4:44370/1‑149 (MQ=255)                                                                                                                         gCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCttt                         <  7:162800/149‑1 (MQ=255)                                                                                                                                gggTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAAc                   >  3:351737/1‑148 (MQ=255)                                                                                                                                  gTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAc                <  4:351737/149‑1 (MQ=255)                                                                                                                                   tACCGTCACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc               >  2:139288/1‑149 (MQ=255)                                                                                                                                      cGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGc                                   <  4:12842/126‑1 (MQ=255)                                                                                                                                      cGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGc                                   >  3:12842/1‑126 (MQ=255)                                                                                                                                        acacCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGAAGGCTTTGGCAACGACCtcag           >  8:282042/1‑145 (MQ=255)                                                                                                                                          cccatGATCAGGGTGGCCTTCAACGACTTCCTAAACTGTTCGGCAGGTAACGTTACGGTGGTGGGTGGGGCCGGTAACTAAACCATGATCGCCACCGATGGAAACTATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat        <  3:73465/145‑1 (MQ=255)                                                                                                                                          acCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGaaa                                                     >  2:213956/1‑104 (MQ=255)                                                                                                                                          acCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGaaa                                                     <  1:213956/104‑1 (MQ=255)                                                                                                                                             cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCaa     >  5:208611/1‑149 (MQ=255)                                                                                                                                                aTCAGGGTGTCCGTCAACGACTTCCTCAACGGTTCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAttt  <  5:287275/149‑1 (MQ=255)                                                                                                                                                aTCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAttt  >  8:206171/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATTT  >  NC_002947/2921314‑2921605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑