Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 353,067 A→G intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947353,0670AG98.1% 175.2 / NA 53intergenic (+34/+84)hisF/cbcVimidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (27/25);  minor base T (0/1);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGA  >  NC_002947/352921‑353200
                                                                                                                                                  |                                                                                                                                      
ttCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCggg                                                                                                                                      <  2:180524/149‑1 (MQ=255)
    ggAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACg                                                                                                                                  >  7:179149/1‑149 (MQ=255)
      aaGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGcc                                                                                                                                <  1:251566/149‑1 (MQ=255)
            ccgCCAGCACCGCGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTcc                                                                                                                          >  7:293868/3‑149 (MQ=255)
                 aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                                     <  8:317785/149‑1 (MQ=255)
                     cgGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGttt                                                                                                                 >  4:232236/1‑149 (MQ=255)
                       gTGCTGGCGGCCAGCATCTTCCACTTTGGCTAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGGTTGc                                                                                                               <  2:220227/149‑1 (MQ=255)
                       gTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGc                                                                                                               <  4:362079/149‑1 (MQ=255)
                           tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTg                                                                                                             <  8:312631/147‑1 (MQ=255)
                           tGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTg                                                                                                             >  7:312631/1‑147 (MQ=255)
                                  cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCtg                                                                                                    >  8:227927/1‑149 (MQ=255)
                                  cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCtg                                                                                                    >  2:339993/1‑149 (MQ=255)
                                    gCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCtgtg                                                                                                  <  3:42980/149‑1 (MQ=255)
                                     cATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTgg                                                                                                 >  6:134915/1‑149 (MQ=255)
                                       tCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTgg                                                                                                 >  7:20981/1‑147 (MQ=255)
                                                   gCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGccc                                                                                   <  7:227927/149‑1 (MQ=255)
                                                          cacaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCtgt                                                                                                   <  7:362946/126‑1 (MQ=255)
                                                          cacaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCtgt                                                                                                   >  8:362946/1‑126 (MQ=255)
                                                           acaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGCTGTCTGTGGGAGCGGGCGGGCCCGCGAAggg                                                                           >  8:256737/1‑149 (MQ=255)
                                                             aGTGACGGAGGCCAAGGCCTACATCGCTTCGCGCGGGATCGTAGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCGCCCCCACGGTTGCTGCGATCTCTGTGGGAGAGGGCGGGCCCGCGCAGGGcc                                                                         >  6:402088/1‑149 (MQ=37)
                                                               tGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCcg                                                                        <  8:20981/148‑1 (MQ=255)
                                                                   ggAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAg                                                                   >  6:407587/1‑149 (MQ=255)
                                                                        ccAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGcc                                                             <  8:293868/149‑1 (MQ=255)
                                                                         catggCCTAAATGGCGTCGCGCGGGTGCGGGGTTCGCTTATCCATTCCCGGGGGCGGCTTTCCCGCCGTGCGGGGGCACGCCCGCCCCCACCGGTTGGCTGAGATCTCTGGGGGAGCGGGGGTGCCCGCGAAGGGCCGCGAAGC‑GGccc                                                            <  4:160514/146‑1 (MQ=255)
                                                                          aaGGCCTACATGGCTTCGCGCGGCAGCGCCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCAAAGC‑AGcccc                                                           <  2:319520/149‑1 (MQ=37)
                                                                            ggCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCaa                                                         >  7:233652/1‑149 (MQ=255)
                                                                             gCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCCCCCGCTCCCCCAGGTTTGGGGAGGTGTCTGTGGGGGCGGGGGTGGCCGCGCAGGGCCGCGCAGCGGGCCCCaa                                                         >  1:380802/1‑149 (MQ=25)
                                                                                 aCATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTg                                                                                                             <  2:384040/93‑1 (MQ=255)
                                                                                  cATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCTGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCt                                                    <  7:256737/148‑1 (MQ=255)
                                                                                  cATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCt                                                    >  2:34551/1‑148 (MQ=255)
                                                                                  cATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGGTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCtt                                                   >  2:40576/1‑149 (MQ=255)
                                                                                     ggCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCACCCCCAGGTTTGCTGAGATCTATGTTGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGGCCCCATTGCTTaaa                                                >  8:229632/1‑149 (MQ=38)
                                                                                        ttCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCGCCCCCAGGTTTGGTGTGATCTGTGTGGGGGCGGGGGGGCCCGCGCAGGGCCCCGCAGC‑GGGCCCCATTGGTTAAAccc                                             >  7:148937/1‑148 (MQ=25)
                                                                                           gcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTg                                           <  5:134915/148‑1 (MQ=255)
                                                                                           gcgcgGGATCGTCGTTCGCTGAGCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTgg                                          <  5:378084/149‑1 (MQ=255)
                                                                                            cgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCGCCCCCACGTGTGGTGTGATAACGGTGGGGGCGGGGGGGGCCGCGCAGGGCCGGCGCGC‑GGCCCCAAATGCTTCAAACGTggg                                         >  8:146451/1‑148 (MQ=255)
                                                                                             gcgGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGCTCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGtt                                        <  8:179149/149‑1 (MQ=255)
                                                                                                            gCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATGTCTGTGGGAGCGGGCGGGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAgcgc                         >  2:109133/1‑149 (MQ=255)
                                                                                                             cttaTCCATTGCCCGGGTCTGCTTTGCAGCCCTTCGCGGCCACGCCCGCTCCCACTGGTTTGCTGAGATCTCTGTGGGTGCGGGGGGGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGcgcg                        <  5:330421/146‑1 (MQ=38)
                                                                                                              tGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGt                       >  7:341422/1‑149 (MQ=255)
                                                                                                              tGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCGGAGATCTCTGTGGGAGCGGGCGTGCCCGCGCAGGGCCGCGAAGC‑GGCCCCAATTGCTTGAACCGTGGTTCTTGCCGCGCAGCGCGt                       >  3:141712/1‑149 (MQ=38)
                                                                                                                   cttggcccGGGGCTGCTTTGCAGCCCTGCGCGGGCAGGCCCGCTCCCACCGGTTGGGTGAGAGCTCTGGGGGAGCGGGGGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTaa                  <  7:146451/143‑1 (MQ=37)
                                                                                                                   cATTGACGGGGGCTGCTTTGCAGCCCTGCGCGGGCACGCCCGCGCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTaa                  <  7:196092/149‑1 (MQ=255)
                                                                                                                    aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCtt                                     >  5:299361/1‑129 (MQ=255)
                                                                                                                    aTTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCtt                                     <  6:299361/129‑1 (MQ=255)
                                                                                                                       gACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCt              >  8:1672/1‑149 (MQ=255)
                                                                                                                        cccGGGGGTGCTTGCCAGCCCTCCGCGGCCCCGCCCGCTCCCACTGGTTTGCTCATATCCCTGGGGGAGGGGGCGGGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCt              <  5:35037/147‑1 (MQ=37)
                                                                                                                        aCCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTGTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGGTTCAACCGGGGTTGTTGCCGAGCAGCGCGTGGTAAAGCTc             >  7:281764/1‑149 (MQ=38)
                                                                                                                         ccGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCg            <  7:1672/149‑1 (MQ=255)
                                                                                                                         ccGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCg            <  1:339993/149‑1 (MQ=255)
                                                                                                                         ccGGGGCGCGTTTGCCGCCCTTCGCGGGCACCCCCGCTCCCCCCGGTTTGCTGCGCTCTCTGTGGGCGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCg            <  8:281764/149‑1 (MQ=37)
                                                                                                                               ctgctTTGCAGCCCTTCGCGGGCACCCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCCCGCAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAATCTCCGTATcc      >  8:200863/1‑149 (MQ=38)
                                                                                                                                tgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATccc     >  8:254259/1‑149 (MQ=255)
                                                                                                                                     tGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGa  <  8:233652/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                      
TTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGC‑GGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGA  >  NC_002947/352921‑353200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑