Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,948,277 C→T R66H (CGT→CAT)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,948,2770CT80.4% 110.8 / 22.5 56R66H (CGT→CAT) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base C (7/4);  new base T (24/21);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGG  >  NC_002947/5948138‑5948421
                                                                                                                                           |                                                                                                                                                
ggCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGgaaac                                                                                                                                         >  2:394787/1‑149 (MQ=255)
 gCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGgaaacc                                                                                                                                        >  2:144603/1‑149 (MQ=255)
 gCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGgaaacc                                                                                                                                        >  3:122908/1‑149 (MQ=255)
     aGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGgaaaccgaaa                                                                                                                                    <  7:329989/149‑1 (MQ=255)
       tAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGgaaaccgaaac                                                                                                                                   >  8:357089/1‑148 (MQ=255)
         aCGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCAt                                                                                                                                >  7:3215/1‑149 (MQ=255)
           gCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATcc                                                                                                                              >  2:160868/1‑149 (MQ=255)
              cccaTTCATTCGGTGGGCGGATCTTGCCGACGATGGTGTAGCCGGTACGAAGCTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCCGCCAGGTAGCACGCATCCGCCGAATGCAAGAAACCGAaaccaacc                                                                                                                              >  8:364562/1‑146 (MQ=255)
               cccTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTg                                                                                                                            >  2:144386/1‑147 (MQ=255)
               cccTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGga                                                                                                                          <  1:144603/149‑1 (MQ=255)
                 cttccttcGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGgag                                                                                                                         >  3:372734/1‑148 (MQ=255)
                         ggTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCa                                                                                                                 <  1:144386/148‑1 (MQ=255)
                                  aTCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTc                                                                                                       >  3:15408/1‑149 (MQ=255)
                                       gCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTc                                                                                                       <  7:357089/144‑1 (MQ=255)
                                       gCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTc                                                                                                       <  7:2953/144‑1 (MQ=255)
                                       gCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTc                                                                                                       <  4:122908/144‑1 (MQ=255)
                                       gCCGACGATGGGGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCAccc                                                                                                   <  3:50963/148‑1 (MQ=255)
                                                        cGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCt                                                                                                                             >  7:62399/1‑105 (MQ=255)
                                                        cGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCt                                                                                                                             <  8:62399/105‑1 (MQ=255)
                                                        cGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGAtct                                                                                            >  2:200255/1‑138 (MQ=255)
                                                        cGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGAtct                                                                                            <  1:200255/138‑1 (MQ=255)
                                                              gCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCa                                                                           >  5:72636/1‑149 (MQ=255)
                                                               cAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCAt                                                                          <  1:160868/149‑1 (MQ=255)
                                                                 ggTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGc                                                                        <  6:72636/149‑1 (MQ=255)
                                                                   ttGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGctt                                                                      >  2:17120/1‑149 (MQ=255)
                                                                        aCGGCGGATCTGGCTGGGCGAGACATATATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCgag                                                                                                <  8:85790/118‑1 (MQ=255)
                                                                        aCGGCGGATCTGGCTGGGCGAGACATAGATATTGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGcttctt                                                                   >  6:54233/1‑147 (MQ=255)
                                                                        aCGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCgag                                                                                                >  7:85790/1‑118 (MQ=255)
                                                                           gcggATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGTCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTcagca                                                              <  8:3215/149‑1 (MQ=255)
                                                                              gATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAggg                                                           >  2:309598/1‑149 (MQ=255)
                                                                                   ggctggGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGaa                                                       >  3:134968/1‑148 (MQ=255)
                                                                                    gctggGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGaa                                                       <  1:17120/147‑1 (MQ=255)
                                                                                        ggCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCAc                                                 <  1:394787/149‑1 (MQ=255)
                                                                                               cATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGt                                          <  4:134968/149‑1 (MQ=255)
                                                                                                    atatCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAAt                                                    <  2:107337/134‑1 (MQ=255)
                                                                                                    atatCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAAt                                                    >  1:107337/1‑134 (MQ=255)
                                                                                                         gTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGaa                                 <  1:309598/148‑1 (MQ=255)
                                                                                                                 cggccAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATg                        >  8:128628/1‑149 (MQ=255)
                                                                                                                     cAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGtttt                    >  6:354723/1‑149 (MQ=255)
                                                                                                                     cAGGTAGGACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGtttt                    >  6:382192/1‑149 (MQ=255)
                                                                                                                        gTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCg                  >  5:273087/1‑148 (MQ=255)
                                                                                                                           ggACGCATCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGc              <  5:382192/149‑1 (MQ=255)
                                                                                                                            gACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGcc             >  1:16821/1‑149 (MQ=255)
                                                                                                                            gACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGcc             >  3:329304/1‑149 (MQ=255)
                                                                                                                             actcATCAGCCGAATGCAGCAAACCGACACCATCCTGTAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCTCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGccc            <  8:148301/146‑1 (MQ=255)
                                                                                                                              tattccAGTCGAATGCAGTACACCGAAACCATCCTGGAGAATCTCCAGAACGCCGTCAGCCGAGATCTCTTCGCCGCTTTTCGCATTCTTCTTAAGCAGGGAGAAAATCACGTCCTGTTTGAGCGAACGTGCCATGTTTTCGATGCCCa           <  6:226752/144‑1 (MQ=255)
                                                                                                                                 aTCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCt        >  5:212635/1‑149 (MQ=255)
                                                                                                                                 aTCAGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCt        >  8:325456/1‑149 (MQ=255)
                                                                                                                                  tCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCCTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTg       >  2:329932/1‑149 (MQ=255)
                                                                                                                                   cAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAAt                                                    <  2:2861/103‑1 (MQ=255)
                                                                                                                                    aGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGtt     <  4:372734/149‑1 (MQ=255)
                                                                                                                                    aGCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGtt     >  3:253204/1‑149 (MQ=255)
                                                                                                                                     gCCGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTAGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTc    >  5:66126/1‑149 (MQ=255)
                                                                                                                                      ccGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCg   >  6:162044/1‑149 (MQ=255)
                                                                                                                                      ccGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCg   <  4:253204/149‑1 (MQ=255)
                                                                                                                                       cGAATGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCgg  <  5:354723/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                
GGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAACCGAAACCATCCTGGAGAATCTCCAGCACGCCGTCACCCGAGATCTCTTCGCCGCTTTTCGCATGCTTCTTCAGCAGGGCGAAAATCACGTCCTGTTTGCGCGAACGGGCCATGTTTTCGATGCCCATCTGTTCGG  >  NC_002947/5948138‑5948421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑