Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 6,013,885 T→C intergenic (+90/+20) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,013,8850TC97.7% 141.6 / NA 44intergenic (+90/+20)xpt/PP_5266xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (21/22);  minor base A (1/0);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATC  >  NC_002947/6013740‑6014027
                                                                                                                                                   |                                                                                                                                              
aaTCGCGGGCCCGGGGGAAGGCGCGGGATGGGGGGGTGGTCAGCTTCATCGAATAAACGCGACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGc                                                                                                                                              <  8:160967/148‑1 (MQ=14)
      tGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCT‑GCTTCGC‑GGGCCCCCCCCCTCCCCCCGGTGCAGCACAGGGGTCGGAAGCGGTGCCGTTCCTGTGGGAGCGGGCGTGGCCGCGAATTGc                                                                                                                                       >  7:244044/1‑149 (MQ=255)
                    tCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCT‑GCTTCGC‑GGGCACCCCCCCACCCCCCGGTGCCGCACAGGTGTGGAACCGGGTGGGGGACCTGTGGGGGCGGGCGGGCCCGCGAATTGCCCCCAccagtttt                                                                                                                          >  8:364859/1‑142 (MQ=255)
                     cGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAACCGCGACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATc                                                                                                                        <  3:172477/149‑1 (MQ=255)
                      gCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGGCTTCGC‑GGGCACGCCCCCACCCACAGGTGCAGCACAGGTAGCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGGGCCCGCGCATGGCCg                                                                                                                                     >  1:88167/1‑136 (MQ=255)
                      gCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCg                                                                                                                                     <  2:88167/135‑1 (MQ=255)
                          gAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCT‑GCTTCGC‑GGGCACCCCCGCTCCCACAGGTGCAGCACAGGTGTCGGACGCGGGGCCCGACCTGTGGGGGCGGGGGTGCCCCCGAATGGCCGCCACCCTGTTATATGGtt                                                                                                                   >  4:227700/1‑149 (MQ=255)
                              gtggtgtggtCAGCTTCATCGAATAAACGCGACACCT‑GCTTCGCGGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATTGCCGCAACCCATTTATATGGTTgcc                                                                                                                >  1:280170/1‑149 (MQ=38)
                               tggtgtggtCAGCTTCATCGAATAAACGCGACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgccgc                                                                                                              <  1:311114/149‑1 (MQ=255)
                                             tCATCGAATAAACGCGACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaac                                                                                                 >  5:300528/1‑148 (MQ=255)
                                                  gAATAAACGCGACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaacaa                                                                                            <  6:300528/148‑1 (MQ=255)
                                                       aaCGCGACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                                      >  5:85202/1‑149 (MQ=255)
                                                          gcgACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCAGTGCCGGACCGGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTggg                                                                                    >  7:309678/1‑147 (MQ=255)
                                                            gACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTaaa                                                                                 <  3:227700/149‑1 (MQ=255)
                                                                     cTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttctt                                                                           <  7:172014/147‑1 (MQ=255)
                                                                        cgc‑gGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTcttctt                                                                        <  2:280170/147‑1 (MQ=255)
                                                                        cgc‑gGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCttt                                                                       <  8:244044/148‑1 (MQ=255)
                                                                             ggCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCAGCGAATGGCCGCAGCCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGGTCTGCTATAAGcc                                                                  >  4:332683/1‑149 (MQ=38)
                                                                                  gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTCCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                                             <  1:166886/149‑1 (MQ=255)
                                                                                      gCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCt                                                         >  7:361879/1‑149 (MQ=255)
                                                                                             caGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCaa                                                  >  3:126315/1‑149 (MQ=255)
                                                                                                gTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgccgcc                                                                                                             <  5:275368/87‑1 (MQ=255)
                                                                                                gTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgccgcc                                                                                                             >  6:275368/1‑87 (MQ=255)
                                                                                                            tctcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAg                                   <  6:85202/149‑1 (MQ=255)
                                                                                                                gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg                               >  3:14252/1‑149 (MQ=255)
                                                                                                                gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg                               >  6:368628/1‑149 (MQ=255)
                                                                                                                gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg                               >  8:321410/1‑149 (MQ=255)
                                                                                                                gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCAGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTATAAGCCCCTGCGGCTGCCCAAACTGCATCCGcccaacgagg                               >  4:355527/1‑142 (MQ=39)
                                                                                                                   cGCTGTGCGGTACGTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCtt                            <  5:256661/149‑1 (MQ=255)
                                                                                                                   cGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGc                                        <  8:321356/137‑1 (MQ=255)
                                                                                                                   cGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGc                                        >  7:321356/1‑137 (MQ=255)
                                                                                                                    gCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCttt                           <  4:14252/149‑1 (MQ=255)
                                                                                                                        tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa                         <  5:368628/147‑1 (MQ=255)
                                                                                                                         gCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAc                      >  7:153740/1‑149 (MQ=255)
                                                                                                                          ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAc                      <  8:361879/148‑1 (MQ=255)
                                                                                                                           cGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc                    <  7:364859/149‑1 (MQ=255)
                                                                                                                            gTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAcccc                   <  4:387293/149‑1 (MQ=255)
                                                                                                                                      gAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTAACTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCg         >  6:121865/1‑149 (MQ=255)
                                                                                                                                        gCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGt       >  1:110276/1‑149 (MQ=255)
                                                                                                                                          gggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAc                      >  2:174989/1‑132 (MQ=255)
                                                                                                                                          gggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAc                      <  1:174989/132‑1 (MQ=255)
                                                                                                                                            gCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                                                       <  3:348108/97‑1 (MQ=255)
                                                                                                                                            gCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                                                       >  4:348108/1‑97 (MQ=255)
                                                                                                                                             cGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATc  <  3:265097/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
AATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCT‑GCTTCGC‑GGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATC  >  NC_002947/6013740‑6014027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑