Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,819	2 bp→CT	intergenic (+56/+696)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,819	0	T	C	100.0%	131.2 / NA	41	intergenic (+56/+697)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/16);  total (25/16)
*	NC_002947	4,740,820	0	G	T	100.0%	133.3 / NA	41	intergenic (+57/+696)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/16);  total (25/16)

CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA  >  NC_002947/4740679‑4740954                                                                                                                                             ||                                                                                                                                        cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGACAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                                    <  7:132311/146‑1 (MQ=255) cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                 >  3:146241/1‑149 (MQ=255)       gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                           <  4:146241/149‑1 (MQ=255)       gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                           >  8:21417/1‑149 (MQ=255)        tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcg                                                                                                                          >  3:34518/1‑149 (MQ=255)           aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTCCGGCCTATTAGCgg                                                                                                                       >  8:266204/1‑149 (MQ=255)           aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                       >  1:33110/1‑149 (MQ=255)           aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGCGCCGGCCTCTTCGCgg                                                                                                                       <  4:239918/149‑1 (MQ=255)               tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                                   <  5:268798/149‑1 (MQ=255)               tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                                   <  7:354392/149‑1 (MQ=255)                     gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                   <  4:187665/127‑1 (MQ=255)                     gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                   >  3:187665/1‑127 (MQ=255)                      tCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcg                                                                                                                          >  4:242238/1‑135 (MQ=255)                      tCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcg                                                                                                                          <  3:242238/135‑1 (MQ=255)                                               cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaa                                                                                    >  1:4695/1‑148 (MQ=255)                                                 gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACCAAAc                                                                                 >  7:301840/1‑149 (MQ=255)                                                  aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                          >  5:177125/1‑123 (MQ=255)                                                  aaGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                          <  6:177125/123‑1 (MQ=255)                                                       cgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGt                                                                           >  1:233243/1‑149 (MQ=255)                                                                   ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                               <  2:233243/149‑1 (MQ=255)                                                                     tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccgc                                                             >  6:199640/1‑149 (MQ=255)                                                                     tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccgc                                                             >  2:214069/1‑149 (MQ=255)                                                                     tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCATAATCGCGGCCATGGCCCGGAAGAGGCCGGAACAGACAACACAAAACCTAAGTTTTTATcagcccc                                                              >  7:53636/1‑147 (MQ=255)                                                                          taCCGAAAGTAAGCTGCAGTAGCCTAACGAAAGAAGGCTGCCCTCGGGCTG‑CTTTTTTTATTTGTCCTGTGCCGGCCTATTCGCGGCTTTGCCCGGTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑Cg                                                       <  2:4695/148‑1 (MQ=255)                                                                               cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTCATACCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGATTTTATCCGCCCCCTCA‑CGCAccc                                                  >  3:262538/1‑149 (MQ=255)                                                                                          cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTa                                       <  1:214069/149‑1 (MQ=255)                                                                                             tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTAtt                                     >  1:88495/1‑148 (MQ=255)                                                                                                ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGAATACCGGGCGTGGCCCCCCAGAGGCCGGCCCCCACACCCCAAAAACGCACGTTTTGTACCCCCCCACACCCCACCCCTTGAAGGGTAttaa                                   >  4:186369/1‑146 (MQ=255)                                                                                                    aCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTGTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGgcg                             >  1:231961/1‑149 (MQ=255)                                                                                                    aCGCAACCAGGCGGCCCGCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGgcg                             <  8:301840/149‑1 (MQ=255)                                                                                                     ataaaaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCGTTCAGGGTATTGAGCGgcgc                            >  6:374020/7‑149 (MQ=255)                                                                                                      taaaaaagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCa                           >  5:101565/6‑149 (MQ=255)                                                                                                            agGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                      >  3:187563/1‑148 (MQ=255)                                                                                                             ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca                    >  5:158922/1‑149 (MQ=255)                                                                                                                 gCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                                 <  7:86844/85‑1 (MQ=37)                                                                                                                 gCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                                 >  8:86844/1‑85 (MQ=37)                                                                                                                     tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGCAGAGGCCGGGCCACACCCCCCAAAACCTCAGTTTTTATACGCCCCCCCA‑CGCCCCCCGTTGAGGGTATTGAACGGCGCATCCACCACCAACttt            >  6:19833/1‑148 (MQ=255)                                                                                                                        ggCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg         <  6:101565/149‑1 (MQ=255)                                                                                                                         atgtgccttttttATTTGTCCTGGGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg         <  5:37796/142‑1 (MQ=255)                                                                                                                          cAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGc       <  2:33110/149‑1 (MQ=255)                                                                                                                                ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGGGCGGCACAGTCACCACAAAACCGCAGTTTTTGTCCGCCCGGTCA‑CGCAGCCCTTTGAGGGTATTGAACGGGGGCTGCACCACGCACTTGTTGGccacca  >  3:367749/1‑149 (MQ=255)                                                                                                                                             ||                                                                                                                                        CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCA‑CGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA  >  NC_002947/4740679‑4740954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑