| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 95.3% | 116.3 / ‑4.1 | 43 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (20/21); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 97.7% | 124.9 / ‑6.7 | 43 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (21/21); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > NC_002947/1499339‑1499611 || cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCtt > 7:291172/1‑149 (MQ=255) gACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGAAACAGGCGCCCCGGGGCATGGCCCC‑GGCgtt > 8:18262/1‑149 (MQ=255) gggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGcc < 2:123541/149‑1 (MQ=255) gggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGcc > 3:102650/1‑149 (MQ=255) ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgt < 4:278027/149‑1 (MQ=255) ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgt < 4:102650/149‑1 (MQ=255) gCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCCTGGCCGCGAAAAAGGCGCCGCGGTGCAATGCACC‑CGCATTGCCGctgt > 2:107698/1‑149 (MQ=255) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCAcg‑c < 1:114300/144‑1 (MQ=37) cgCCCGTTATGCGGCCATTCAGAGCCTTAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCAcg‑cccgctc < 8:291172/148‑6 (MQ=21) cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGa > 7:375680/1‑149 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCGTTTCCGGTGTTAGCGGGCACGCCCGCTCCCACAGACGGATTGGCATTAAGCgggg > 4:81223/1‑148 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 8:375680/148‑1 (MQ=21) gCCTTGGCACCACATCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgtg > 7:339371/1‑96 (MQ=255) gCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgtg < 8:339371/96‑1 (MQ=255) gCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGGGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc < 8:313515/149‑1 (MQ=25) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGttcg < 3:260006/92‑3 (MQ=255) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGttcg > 4:260006/1‑90 (MQ=255) cagcagGACTGAGGCTGCCAAACGGGGGGAGCGGGCGTCCCCGCTAAACAGGCCCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc < 3:225294/149‑1 (MQ=25) agccgGACTGAGGCTGCCAACCGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGAACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc < 5:210461/149‑1 (MQ=21) agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGAGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc > 8:31610/1‑149 (MQ=21) tggCTGCCAAACGGGGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 8:152567/146‑1 (MQ=21) cggCTGCCAAAGGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACG‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 3:242834/146‑1 (MQ=21) ggCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 7:18262/149‑1 (MQ=25) cttccAAACGGTGGGAGGGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACG‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc < 3:81223/146‑1 (MQ=25) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 5:290591/141‑1 (MQ=21) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt > 6:290591/1‑141 (MQ=21) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑CGCTTTGCCGGTGTTAGCGGGCCCGCCCGCTCCCACAGACTGATTTCCATTAAGCCGGCCTATCGCTACAGTTCCGGCCCGGTTTTTGTTGCCtg > 6:298543/1‑148 (MQ=18) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc > 1:293549/1‑149 (MQ=21) gAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 5:298543/149‑1 (MQ=25) cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTAGCGGGCACGCCCGCTCCCCCAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt > 4:168697/1‑148 (MQ=25) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACT‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt < 1:314069/110‑1 (MQ=18) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACT‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt > 2:314069/1‑110 (MQ=18) gTGCCCGCAAAACCGCCGCCGCGGTGCAT‑GCCACGGGCTTTGCGGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc < 7:140856/149‑1 (MQ=37) tGCCCGCGAACCAGGCGCCGCGGGGCATGGAACC‑GGGTTTGCTGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc < 7:276061/149‑1 (MQ=25) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 6:23022/1‑149 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 7:199125/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAAc < 8:180296/132‑1 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAAc > 7:180296/1‑132 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 4:323066/1‑146 (MQ=37) cgAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa > 3:119209/1‑149 (MQ=37) gcgcCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt > 4:327541/1‑137 (MQ=37) gcgcCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt < 3:327541/137‑1 (MQ=37) cgGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 8:306210/1‑145 (MQ=37) || CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > NC_002947/1499339‑1499611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |