| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,429 | Δ1 bp | 6.2% | intergenic (‑47/+42) | trmE ← / ← oxaA | GTPase/membrane protein insertase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,429 | 0 | A | . | 6.2% | 290.0 / 13.6 | 97 | intergenic (‑47/+42) | trmE/oxaA | GTPase/membrane protein insertase |
| Reads supporting (aligned to +/- strand): ref base A (42/49); new base . (4/2); total (46/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01 | |||||||||||
GCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTACAGCACCAGACCGG > NC_002947/6281‑6574 | gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa > 1:72871/1‑149 (MQ=255) ggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGAAAAAACGcaccac > 4:219595/1‑147 (MQ=255) ccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑g > 3:201105/1‑149 (MQ=255) ccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑g < 5:278700/149‑1 (MQ=255) ccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑g < 1:391075/149‑1 (MQ=255) cTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑gg > 8:96487/1‑149 (MQ=255) ggCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑ggg < 3:340436/147‑1 (MQ=255) ggCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGc > 4:344695/1‑149 (MQ=255) aGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑tt < 4:404754/149‑1 (MQ=255) ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑tttt < 3:219595/149‑1 (MQ=255) ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑tttt < 4:1696/149‑1 (MQ=255) cAGAGGGCCGGACAACCTGACAATTCCCACCCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATCGCAAAAACGCCCC‑CTAGTGGGGCG‑ttttt < 1:404979/149‑1 (MQ=255) cAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑ggg > 6:391373/1‑141 (MQ=255) cAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑ggg < 5:391373/141‑1 (MQ=255) gagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTa < 2:86158/149‑1 (MQ=255) gagGGCCGGACAACCTGACAATTCCAACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGTCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTa < 5:103239/149‑1 (MQ=255) ccGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTca > 7:251847/1‑148 (MQ=255) ccGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTca > 7:209409/1‑148 (MQ=255) cGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACTCCCCACTAG‑GGGGCG‑TTTTTTATTcac > 4:316850/1‑148 (MQ=255) ggACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAg > 7:132856/1‑149 (MQ=255) gACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAgg > 7:138360/1‑149 (MQ=255) aCAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑TGGGCG‑TTTTTTATTCACAGGc > 2:255772/1‑149 (MQ=255) aaCCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTa < 5:189210/149‑1 (MQ=255) ccTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACTCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGc > 2:370514/1‑149 (MQ=255) ccTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGc < 4:201105/149‑1 (MQ=255) cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCg > 8:19346/1‑149 (MQ=255) cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCg > 1:415285/1‑149 (MQ=255) aCAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGt < 1:424283/147‑1 (MQ=255) cAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTAtt < 2:415285/149‑1 (MQ=255) cAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTAtt < 7:96487/149‑1 (MQ=255) aaTTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTa < 6:404568/149‑1 (MQ=255) ttCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACTGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGc < 2:162245/149‑1 (MQ=255) ttCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGc > 3:156459/1‑149 (MQ=255) ttCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGc < 3:344695/149‑1 (MQ=255) tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGcc > 8:278168/1‑149 (MQ=255) ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGccc < 4:394135/148‑1 (MQ=255) ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGccc < 1:137792/148‑1 (MQ=255) cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCGCGt > 4:134866/1‑149 (MQ=255) cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGt < 8:300968/149‑1 (MQ=255) catcGCCACGGCCCAGGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGCGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTc > 6:442845/4‑148 (MQ=255) cacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCa < 2:72871/149‑1 (MQ=255) ccGCCCCGGCCCTGGGCCGTGGCGATGGTGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag < 4:209251/149‑1 (MQ=255) ccGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag > 6:68411/1‑149 (MQ=255) ccccGTCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag > 4:229897/1‑149 (MQ=255) ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag > 3:54022/1‑149 (MQ=255) cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagc < 3:316850/149‑1 (MQ=255) ggCCGTGGCGATGGCGGCGATGGTTTCACTCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCtttttt < 1:370514/148‑1 (MQ=255) ggCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCttttt < 4:156459/147‑1 (MQ=255) cGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTa < 1:51497/149‑1 (MQ=255) tGGCGATGGCGGCGATGGGTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACTCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAgc < 7:355860/149‑1 (MQ=255) cGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTTATTCACAGGCTAGTGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAgctg < 1:336757/149‑1 (MQ=255) cGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAgctgc < 8:209409/149‑1 (MQ=255) gATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAgctgct > 5:416919/1‑149 (MQ=255) aTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCtt < 7:134473/149‑1 (MQ=255) gTTTCACGCACAGTGTTCATTATCTAAAGCCTATAAGACAAAAAAGACAGATAGCAAAAACGACCCACTAT‑GTGTAG‑TTTTTTATTCACATGCTAGAGTATTAGCCCGTCAAGCAGCATCTTTTTTGGTAGCTGCTTCGATACGGcta < 8:138360/148‑3 (MQ=255) tttCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCAGTAGAGCAACATCTTTTTTTGTAGCTGCTTCGATACGGCGact > 1:363752/1‑147 (MQ=255) ttCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGt > 4:171648/1‑148 (MQ=255) aCGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGAt < 6:416919/148‑1 (MQ=255) aCGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATg < 2:302151/149‑1 (MQ=255) gCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAgc > 2:394300/1‑125 (MQ=255) gCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAgc < 1:394300/125‑1 (MQ=255) gCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTa > 2:252843/1‑149 (MQ=255) acaGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTAc > 7:181118/1‑148 (MQ=255) aGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCAc > 1:50062/1‑149 (MQ=255) tgtTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCActg < 4:54022/149‑1 (MQ=255) ttCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCActgct < 8:132856/149‑1 (MQ=255) ttCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCActgct < 1:223288/149‑1 (MQ=255) aTGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACtgctgc > 1:274628/1‑148 (MQ=255) tGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCg > 5:98080/1‑148 (MQ=255) cTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGaa < 2:425557/148‑1 (MQ=255) cTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGaa < 7:19346/148‑1 (MQ=255) gAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATc < 5:442845/148‑1 (MQ=255) gAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATc > 6:334014/1‑148 (MQ=255) gCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAg < 3:335299/149‑1 (MQ=255) ctctACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAgg > 4:342624/1‑148 (MQ=255) ctACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAg > 6:367992/1‑149 (MQ=255) ctACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAg > 8:137922/1‑149 (MQ=255) ctACGACAAAAACGACAGATAGCAAAAACGCCCCACAAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCCTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGAAACGGCGATTGATGTACCACTGATGCGAAATCTACACGCAg > 8:131757/1‑149 (MQ=255) tACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCa < 3:229897/147‑1 (MQ=255) cGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAgttg < 3:342624/149‑1 (MQ=255) cAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAgttgtt > 7:114959/1‑148 (MQ=255) aaaaaCGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTca > 4:249095/1‑149 (MQ=255) aaaaaCGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTca > 6:123533/1‑149 (MQ=255) aaaaaCGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTca < 5:334014/149‑1 (MQ=255) aaaCGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTA‑TAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACaa < 6:118280/149‑1 (MQ=255) cGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTATCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAAcc < 8:114959/149‑1 (MQ=255) tgaataaaaaaCGCCCC‑CTAGTGGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTa > 8:209398/7‑149 (MQ=255) tAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTAc < 8:181118/148‑1 (MQ=255) aataaaaaaCGCCCC‑CTAGTGGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTa > 1:31412/5‑147 (MQ=255) ataaaaaaCGCCCC‑CTAGTGGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTACAg > 3:193670/4‑149 (MQ=255) gCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTACAg > 7:89241/1‑148 (MQ=255) aaaaaaCGCCCC‑CTAGTGGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTACAGCa < 7:209398/148‑1 (MQ=255) aaaaaCGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTACAGCAc > 6:375266/1‑149 (MQ=255) aaaaCGCCCCACTAG‑GCGGCG‑TTTTTTATTCACAGGCTAGCGAATTAGCCCGTCAAGCAGCAGCTTATTTGCTAGCTGCTTCGATACGGCGAGTGATGTAACACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTAcaccac > 3:128972/1‑148 (MQ=255) aaaaCGCCCC‑CTAGTGGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTACAGCAc > 4:176925/1‑148 (MQ=255) aaaCGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAGCCCAGTACAGCACCa < 1:255772/149‑1 (MQ=255) cccACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTACAGCACCAGACCgg > 2:124613/1‑149 (MQ=255) cACTAT‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTACAGCACCAGACCg < 3:6270/146‑1 (MQ=255) cACTAG‑GGGGCG‑TTTTTTATTAACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTAACAACCCAGTACAGCAACAGaact > 4:6270/1‑143 (MQ=255) | GCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCG‑TTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAGCTGCTTCGATACGGCGAGTGATGTACCACTGCTGCGAAATCGACAGGCAGTTGTTCACAACCCAGTACAGCACCAGACCGG > NC_002947/6281‑6574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |