Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,740,319 Δ1 bp 7.5% coding (846/1290 nt) gltA → citrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,3190C. 7.5% 210.6 / 9.2 67coding (846/1290 nt)gltAcitrate synthase
Reads supporting (aligned to +/- strand):  ref base C (30/32);  new base . (2/3);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCACCTCCACCGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGC  >  NC_002947/4740175‑4740466
                                                                                                                                                 |                                                                                                                                                    
ctccacctccacCGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGa                                                                                                                                                  <  7:255218/149‑1 (MQ=255)
    acctccaccGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACAt                                                                                                                                             <  4:100269/149‑1 (MQ=255)
    acctccaccGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACAt                                                                                                                                             <  3:67454/149‑1 (MQ=255)
     cctccaccGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATc                                                                                                                                            >  1:265743/1‑149 (MQ=255)
         caccGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGAc                                                                                                                                         >  5:355087/1‑148 (MQ=255)
         caccGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACa                                                                                                                                        <  3:54510/149‑1 (MQ=255)
                 gTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAATATCGACAAATTCATc                                                                                                                                <  4:282674/149‑1 (MQ=255)
                  gcttgCAGGCTCGTCGGGTGCCACCCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTTGGGCCGGTGCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATCAAATCGGCTATGTCTCGAACATCGACAAATTCATCg                                                                                                                               <  8:296866/145‑1 (MQ=255)
                     ggCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCgcc                                                                                                                             >  6:247082/1‑148 (MQ=255)
                      gCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGTTCGAGACATCGCCGATTTCATcca                                                                                                                              <  4:348323/146‑3 (MQ=37)
                         ggCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCgccaaggc                                                                                                                        <  2:133240/149‑1 (MQ=255)
                           cTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGTTCGAGACATCGCCGATTTCATcca                                                                                                                              <  8:345695/141‑3 (MQ=37)
                           cTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGTTCGAGACATCGCCGATTTCATcca                                                                                                                              >  7:345695/1‑139 (MQ=37)
                            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGTTCGAGACATCGCCGATTTCATccagcatg                                                                                                                         >  1:148152/1‑138 (MQ=25)
                            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGTTCGAGACATCGCCGATTTCATccagcatg                                                                                                                         <  2:148152/145‑8 (MQ=25)
                            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAAt                                                                                                                                     <  3:418845/133‑1 (MQ=255)
                            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAAt                                                                                                                                     >  4:418845/1‑133 (MQ=255)
                            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAAGTCATCgccaaggcc                                                                                                                       >  8:295858/1‑147 (MQ=255)
                              gtcgTGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCgccaaggccaag                                                                                                                    >  3:231877/1‑148 (MQ=255)
                               tcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGa                                                                                                                  >  2:1205/1‑149 (MQ=255)
                                      ccAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATc                                                                                                                                >  7:282576/1‑128 (MQ=255)
                                      ccAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATc                                                                                                                                <  8:282576/128‑1 (MQ=255)
                                         aCCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGAc                                                                                                        <  2:265743/149‑1 (MQ=255)
                                                 gCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTc                                                                                                  >  1:229661/1‑147 (MQ=255)
                                                 gCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCaa                                                                                                <  4:390568/149‑1 (MQ=255)
                                                     gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACaaa                                                                                                                                      <  6:132170/107‑1 (MQ=255)
                                                     gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACaaa                                                                                                                                      >  5:132170/1‑107 (MQ=255)
                                                     gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCgc                                                                                                                              <  6:427988/115‑1 (MQ=255)
                                                     gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCgc                                                                                                                              >  5:427988/1‑115 (MQ=255)
                                                      tATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGa                                                                                           <  7:371307/149‑1 (MQ=255)
                                                      tATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGa                                                                                           <  7:391829/149‑1 (MQ=255)
                                                        tCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCgccaaggccaa                                                                                                                     >  7:6564/1‑121 (MQ=255)
                                                        tCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCgccaaggccaa                                                                                                                     <  8:6564/121‑1 (MQ=255)
                                                        tCGCAGACGTTATCGAAGAACTGTGGGTCCAGGCCAAATTCAGTGCGAACGAAGACGTACTGACCATGCTGGATGTAATCGGCGATGTCTCGAACATCGACAAATTCATCGACAAGGCAAAGGACAAGAACGACCCCTTCTAGCTGAtc                                                                                         >  4:70823/1‑148 (MQ=255)
                                                         cgccgcCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGACAAGGCCAAGGACAAGAACGACCCGTTCAAGCGGATgg                                                                                        >  8:290221/1‑149 (MQ=255)
                                                            cgccgGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCCAGCTGATGGGCt                                                                                     >  6:202496/1‑149 (MQ=255)
                                                             gccgGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCtt                                                                                    <  1:98589/149‑1 (MQ=255)
                                                               cgGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCg                                                                                  <  2:290653/149‑1 (MQ=255)
                                                                 gTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGt                                                                                >  1:317769/1‑149 (MQ=255)
                                                                  tATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGt                                                                                >  1:420081/1‑148 (MQ=255)
                                                                    tCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCa                                                                              <  2:342488/148‑1 (MQ=255)
                                                                       ccgcACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCGAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACcgc                                                                          >  7:141271/1‑149 (MQ=255)
                                                                         gcACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCgt                                                                        <  2:229661/149‑1 (MQ=255)
                                                                            gccgtgggccgggccCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCgtg                                                                       >  6:431244/6‑148 (MQ=255)
                                                                                ggggCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTGAAGCTGATGGGCTTCGGTCACCGCGTGTAc                                                                    >  1:116012/1‑146 (MQ=255)
                                                                                ggggCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAg                                                                 <  5:15440/149‑1 (MQ=255)
                                                                                  ggcccggcccACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCCAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGaa                                                               >  7:102704/1‑149 (MQ=255)
                                                                                   gcccggcccACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAAc                                                              >  6:431162/1‑149 (MQ=255)
                                                                                            cACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGAccc                                                      >  8:197359/1‑149 (MQ=255)
                                                                                                      cgaacgaaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCAcc                                            >  6:36195/1‑149 (MQ=255)
                                                                                                          cgaaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGtga                                        <  1:71911/149‑1 (MQ=255)
                                                                                                           gaaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGtgat                                       >  6:376579/1‑149 (MQ=255)
                                                                                                            aaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGtgatg                                      <  5:247082/149‑1 (MQ=255)
                                                                                                               ccGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAg                                   <  7:197359/149‑1 (MQ=255)
                                                                                                                   aCTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGa                               >  8:364174/1‑149 (MQ=255)
                                                                                                                    cTGACCATGCTCGTTGAAATCCGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTTTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGAc                              <  4:238617/149‑1 (MQ=255)
                                                                                                                       aCCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCt                            <  5:431162/148‑1 (MQ=255)
                                                                                                                        ccATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGc                          <  5:431244/149‑1 (MQ=255)
                                                                                                                         cATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGcg                         >  3:33868/1‑149 (MQ=255)
                                                                                                                          aTGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGcga                        <  7:364174/149‑1 (MQ=255)
                                                                                                                             cTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGcgacg                      <  2:317769/148‑1 (MQ=255)
                                                                                                                              tGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGaa                    <  2:116012/149‑1 (MQ=255)
                                                                                                                                gATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCCACGaa                    <  7:392771/147‑1 (MQ=255)
                                                                                                                                   gAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCt               >  3:213457/1‑149 (MQ=255)
                                                                                                                                    aaaTCGGCGTTGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTg              >  6:411107/1‑149 (MQ=255)
                                                                                                                                           cGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGc       >  8:219437/1‑149 (MQ=255)
                                                                                                                                           cGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGc       <  2:420081/149‑1 (MQ=255)
                                                                                                                                                 ctcGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGCGCTTCGGTCACCGCGTGTCCAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTCCGACGAAGTCCTGCGCGAGCTGGGc  >  7:280407/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTCCACCTCCACCGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGGCCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGAACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGC  >  NC_002947/4740175‑4740466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: