BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,098,177	Δ1 bp	6.3%	coding (1215/2091 nt)	acsA‑I ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,098,177	0	G	.	6.3%	305.3 / 13.5	95	coding (1215/2091 nt)	acsA‑I	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base G (46/43); new base . (2/4); total (48/47)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

Only 100 of 101 total aligned reads displayed.

TGTCGACGATCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGT  >  NC_002947/5098029‑5098325

tgtCGACGATCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGgcg                                                                                                                                                      >  5:208636/1‑149 (MQ=255)

  tCGACGATCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCACGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGgcgc                                                                                                                                                     >  6:403758/1‑148 (MQ=255)

     acgaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAAt                                                                                                                                                 >  2:9998/1‑149 (MQ=255)

       gaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCTTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                               >  4:418314/1‑149 (MQ=255)

       gaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                               <  6:279356/149‑1 (MQ=255)

       gaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                               <  7:400941/149‑1 (MQ=255)

        aTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                               >  2:315972/1‑148 (MQ=255)

         tCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCa                                                                                                                                             <  1:9998/149‑1 (MQ=255)

         tCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCATTCGCGCCATGATGGC‑CGAAGGGCa                                                                                                                                             <  3:119081/149‑1 (MQ=255)

           gggCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACTCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCATTCGCGCCATGATGGC‑CGAAGGGCAgg                                                                                                                                           <  8:271932/149‑2 (MQ=255)

           gggCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCATTCGCGCCATGATGGC‑CGAAGGGCAgg                                                                                                                                           <  5:266388/149‑2 (MQ=255)

                    tCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCtgt                                                                                                                                  >  7:284342/1‑149 (MQ=255)

                       ttGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCtgtg                                                                                                                                 >  5:345494/1‑147 (MQ=255)

                         gCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTa                                                                                                                               <  5:55746/147‑1 (MQ=255)

                           cGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTaga                                                                                                                             <  7:162684/147‑1 (MQ=255)

                           cGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTaga                                                                                                                             >  8:162684/1‑147 (MQ=255)

                            gACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGa                                                                                                                          >  4:203843/1‑149 (MQ=255)

                             aCGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGAt                                                                                                                         >  5:298832/1‑149 (MQ=255)

                              cGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATg                                                                                                                        <  5:37148/149‑1 (MQ=255)

                                     gtagtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGAcc                                                                                                                  <  3:395826/148‑1 (MQ=255)

                                     gtagtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCt                                                                                                                 >  4:102936/1‑149 (MQ=255)

                                       agtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtg                                                                                                               >  7:371414/1‑149 (MQ=255)

                                        gtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgt                                                                                                              <  6:208636/149‑1 (MQ=255)

                                         taCCAGTTCCAGGCCTCAGGGTTTATCGGCTCACCCACGGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCACCAGCTGGCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgt                                                                                                              <  5:359110/148‑1 (MQ=255)

                                         taCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgtg                                                                                                             <  5:403758/149‑1 (MQ=255)

                                            cAGTTCCAGGCGTCTCGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCtt                                                                                                          <  4:321488/149‑1 (MQ=255)

                                             aGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACAGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTg                                                                                                         >  5:149796/1‑149 (MQ=255)

                                              gTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                               <  6:56306/110‑1 (MQ=255)

                                              gTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                               >  5:56306/1‑110 (MQ=255)

                                                 ccAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgtg                                                                                                             <  2:86603/141‑1 (MQ=255)

                                                 ccAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgtg                                                                                                             >  1:86603/1‑141 (MQ=255)

                                                  cAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTcgac                                                                                                    >  8:149468/1‑149 (MQ=255)

                                                    ggCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCtt                                                                                                          >  8:388325/1‑141 (MQ=255)

                                                    ggCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCtt                                                                                                          <  7:388325/141‑1 (MQ=255)

                                                     gCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGAt                                                                                                 >  2:153465/1‑149 (MQ=255)

                                                      ccTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATc                                                                                                <  5:6923/149‑1 (MQ=255)

                                                       cTCAGGGTAGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTAG‑GCCATCATGGCGCGAATCGCAGTTGGCGCTGAGTAGAGGATGTTGACCTAGTGCTTGTCGACGATc                                                                                                >  6:173116/1‑148 (MQ=255)

                                                              ttGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCtt                                                                                                                <  7:124110/125‑1 (MQ=255)

                                                              ttGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCtt                                                                                                                >  8:124110/1‑125 (MQ=255)

                                                                 aTCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCtt                                                                                                                >  7:158625/1‑122 (MQ=255)

                                                                 aTCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCtt                                                                                                                <  8:158625/122‑1 (MQ=255)

                                                                 aTCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATAcgc                                                                                      <  3:135616/148‑1 (MQ=255)

                                                                    ggCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGt                                                                                  <  7:286346/149‑1 (MQ=255)

                                                                     gCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCGTGTCGACGATCTTCGATACGCGGGt                                                                                  >  6:123608/1‑148 (MQ=255)

                                                                         acccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGTGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAAt                                                                               <  1:315972/147‑1 (MQ=255)

                                                                         acccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCTGGTAATg                                                                              <  5:173116/148‑1 (MQ=255)

                                                                           ccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTg                                                                                                                                         >  7:228224/1‑87 (MQ=255)

                                                                           ccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTg                                                                                                                                         <  8:228224/87‑1 (MQ=255)

                                                                             accGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCgg                                                                         <  2:45249/149‑1 (MQ=255)

                                                                              ccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCggg                                                                        <  2:9597/149‑1 (MQ=255)

                                                                                     cagcagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTgg                                                                 <  6:298832/149‑1 (MQ=255)

                                                                                     cagcagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTgg                                                                 <  6:149796/149‑1 (MQ=255)

                                                                                      agcagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTggg                                                                >  5:331041/1‑149 (MQ=255)

                                                                                        cagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCa                                                              <  3:203843/149‑1 (MQ=255)

                                                                                         agACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCAc                                                             <  3:102936/149‑1 (MQ=255)

                                                                                           aCGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCggg                                                                        <  3:72951/136‑1 (MQ=255)

                                                                                           aCGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCggg                                                                        >  4:72951/1‑136 (MQ=255)

                                                                                           aCGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGc                                                           >  7:61862/1‑149 (MQ=255)

                                                                                           aCGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGc                                                           >  7:64725/1‑149 (MQ=255)

                                                                                             gCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTaa                                                                                <  8:311825/126‑1 (MQ=255)

                                                                                             gCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTaa                                                                                >  7:311825/1‑126 (MQ=255)

                                                                                             gCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCt                                                         >  3:421178/1‑149 (MQ=255)

                                                                                                     gAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAAc                                                   <  7:149468/147‑1 (MQ=255)

                                                                                                     gAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAg                                                 >  4:104839/1‑149 (MQ=255)

                                                                                                      aaCCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCgg                                                                                    >  4:321484/1‑113 (MQ=255)

                                                                                                      aaCCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAg                                                 >  2:303433/1‑148 (MQ=255)

                                                                                                       aCCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGc                                                >  4:203581/1‑148 (MQ=255)

                                                                                                              gcgcCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAggtggt                                        <  8:64725/149‑1 (MQ=255)

                                                                                                               cgcCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGgtggtg                                       <  3:203581/149‑1 (MQ=255)

                                                                                                                gcCTTCAACATCTTCCTGCCCTTCG‑GCCATCATGGGGCGAATTGCAGTTGGCTCTGTGTAGATGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGgtggtg                                       <  3:116074/148‑1 (MQ=255)

                                                                                                                gcCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGtggtgg                                      <  6:331041/149‑1 (MQ=255)

                                                                                                                  cTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGgcg                                    >  3:129366/1‑149 (MQ=255)

                                                                                                                    tCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAggt                                           >  6:350200/1‑140 (MQ=255)

                                                                                                                    tCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAggt                                           <  5:350200/140‑1 (MQ=255)

                                                                                                                    tCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGcc                                  >  8:361052/1‑149 (MQ=255)

                                                                                                                       aCAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGtt                               <  4:421178/149‑1 (MQ=255)

                                                                                                                       aCAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGtt                               <  3:104839/149‑1 (MQ=255)

                                                                                                                       aCAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGtt                               >  5:54015/1‑149 (MQ=255)

                                                                                                                         aGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTgg                             >  7:36511/1‑149 (MQ=255)

                                                                                                                           ctgcctgcCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGc                            >  6:334912/1‑148 (MQ=255)

                                                                                                                              cctgccCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGc                        >  3:214162/1‑149 (MQ=255)

                                                                                                                                  cccTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGc                    >  7:11885/1‑149 (MQ=255)

                                                                                                                                   ccTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGcc                   >  1:387232/1‑149 (MQ=255)

                                                                                                                                   cattcgcgCCATGATGGC‑CGAAGGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGc                            >  5:225411/8‑140 (MQ=38)

                                                                                                                                   cattcgcgCCATGATGGC‑CGAAGGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGc                            <  6:225411/133‑1 (MQ=38)

                                                                                                                                   cattcgcgCCATGATGGC‑CGAAGGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGcc                   >  5:240845/8‑149 (MQ=38)

                                                                                                                                     ttCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGTCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCg                  >  6:328033/1‑148 (MQ=255)

                                                                                                                                     ttCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGcc                                  <  2:133994/132‑1 (MQ=255)

                                                                                                                                     ttCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGcc                                  >  1:133994/1‑132 (MQ=255)

                                                                                                                                       cG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTa                <  5:328033/148‑1 (MQ=255)

                                                                                                                                           ccATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAg                                             >  3:136412/1‑116 (MQ=255)

                                                                                                                                           ccATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAg                                             <  4:136412/116‑1 (MQ=255)

                                                                                                                                            catcatGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGa           >  2:76483/1‑149 (MQ=255)

                                                                                                                                            catcatGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGa           <  6:54015/149‑1 (MQ=255)

                                                                                                                                            catcatGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGa           >  2:363657/1‑149 (MQ=255)

                                                                                                                                                 tGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGa              <  3:175682/141‑1 (MQ=255)

                                                                                                                                                 tGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGa              >  4:175682/1‑141 (MQ=255)

                                                                                                                                                   gcgcGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGt                                                                    <  7:70882/85‑1 (MQ=255)

                                                                                                                                                     gcgAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCtgt  <  2:387232/149‑1 (MQ=255)

                                                                                                                                                     gcgAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCtgt  <  8:61862/149‑1 (MQ=255)

TGTCGACGATCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGT  >  NC_002947/5098029‑5098325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`