Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,962,486 Δ1 bp 8.2% coding (508/777 nt) PP_2592 ← ferric siderophore ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,962,4860G. 8.2% 150.1 / 7.4 49coding (508/777 nt)PP_2592ferric siderophore ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base . (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

GCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAGGCCTGGCGCAGCGGCTCCAGGGACAGA  >  NC_002947/2962343‑2962628
                                                                                                                                               |                                                                                                                                              
gctgcAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCa                                                                                                                                           >  6:77717/1‑149 (MQ=255)
gctgcAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCa                                                                                                                                           >  7:1181/1‑149 (MQ=255)
    cAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTc                                                                                                                                       >  4:300935/1‑149 (MQ=255)
       tGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAg                                                                                                                                    <  8:115958/149‑1 (MQ=255)
         cacaCGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGgt                                                                                                                                  <  4:194973/149‑1 (MQ=255)
           cacGGCCACCCTTCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGgttg                                                                                                                                <  7:400581/149‑1 (MQ=255)
           cacGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGgttg                                                                                                                                <  2:51300/149‑1 (MQ=255)
              ggCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTc                                                                                                                             <  5:77717/149‑1 (MQ=255)
               gCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTc                                                                                                                             >  5:1935/1‑148 (MQ=255)
                   cccTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACgg                                                                                                                        >  8:101973/1‑149 (MQ=255)
                       gCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCg                                                                                                                    >  7:130666/1‑149 (MQ=255)
                        cATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGGCGAGGTTGTTCAACGGCTc                                                                                                                     <  8:294/147‑1 (MQ=255)
                          tGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTcc                                                                                                                 <  8:130666/149‑1 (MQ=255)
                               acgaTGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCcagcag                                                                                                            <  6:1935/149‑1 (MQ=255)
                                    gCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGa                                                                                                        >  8:214783/1‑148 (MQ=255)
                                      aGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAg                                                                                                     <  1:351985/149‑1 (MQ=255)
                                      aGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAg                                                                                                     <  2:137167/149‑1 (MQ=255)
                                           gAGTTGGCGGCTTCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCTCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGGTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGt                                                                                                <  5:363901/149‑1 (MQ=255)
                                           gAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGt                                                                                                <  3:300935/149‑1 (MQ=255)
                                                   ggcTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGT‑GCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGc                                                                                       >  3:94766/1‑149 (MQ=255)
                                                   ggcTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGT‑GCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGc                                                                                       >  3:100669/1‑149 (MQ=255)
                                                        cAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATg                                                                                   <  7:126338/149‑1 (MQ=255)
                                                         aaaGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATgg                                                                                  >  6:189362/1‑149 (MQ=255)
                                                                 tGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGc                                                                           >  2:422724/1‑148 (MQ=255)
                                                                          ccaccaAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGc                                                                  >  6:140583/1‑148 (MQ=255)
                                                                           caccaAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCa                                                                >  8:6369/1‑149 (MQ=255)
                                                                           caccaAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCa                                                                >  5:111149/1‑149 (MQ=255)
                                                                             ccaAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCAtt                                                              >  2:33587/1‑149 (MQ=255)
                                                                              caAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCAttt                                                             >  4:419399/1‑149 (MQ=255)
                                                                                     aCCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCt                                                       <  5:189362/148‑1 (MQ=255)
                                                                                     aCCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTg                                                      >  2:295082/1‑149 (MQ=255)
                                                                                     aCCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTg                                                      >  6:310812/1‑149 (MQ=255)
                                                                                              ccGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCt                                             >  2:158366/1‑149 (MQ=255)
                                                                                               cGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGT‑GCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGa                                           <  4:100669/149‑1 (MQ=255)
                                                                                               cGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGT‑GCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGa                                           <  4:94766/149‑1 (MQ=255)
                                                                                                  aCTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGt                                         >  2:17003/1‑149 (MQ=255)
                                                                                                         gCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTg                                   <  7:214783/148‑1 (MQ=255)
                                                                                                          cacaGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAg                                 >  5:311507/1‑149 (MQ=255)
                                                                                                             aGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTgg                                                     <  5:153839/126‑1 (MQ=255)
                                                                                                             aGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTgg                                                     >  6:153839/1‑126 (MQ=255)
                                                                                                              gCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAg                             <  5:140583/149‑1 (MQ=255)
                                                                                                              gCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAg                             <  7:6369/149‑1 (MQ=255)
                                                                                                                      aGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAGGCCTGgcg                     >  1:63795/1‑149 (MQ=255)
                                                                                                                      aGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAGGCCTGgcg                     >  6:336596/1‑149 (MQ=255)
                                                                                                                        ggCGCGCATGATCAGCACGTCGTGGGGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAGGCCTGGCGCa                   <  4:294207/149‑1 (MQ=255)
                                                                                                                                   tCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAGGCCTGGCGCAGCGGCTCCAgg        >  2:185269/1‑149 (MQ=255)
                                                                                                                                   tCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAGGCCTGGCGCAGCGGCTCCAg         <  5:310812/148‑1 (MQ=255)
                                                                                                                                        aCGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAGGCCTGGCGCAGCGGCTCCAGGGa      >  5:186228/1‑146 (MQ=255)
                                                                                                                                         cGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAGGCCTGGAGCCGCTGCGCCAggcctacc  <  7:373608/149‑7 (MQ=255)
                                                                                                                                               |                                                                                                                                              
GCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCTCGTCCAGCAGCAGATAGTCGGTCTGTTGGGCGATGGTCATGGCCAGAAAGGCCATTTGCCGCTGGCCGCCGCTGAGTTCATTGAGGTAGGCCTGGCGCAGCGGCTCCAGGGACAGA  >  NC_002947/2962343‑2962628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑