Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,324,985 Δ1 bp 6.1% coding (149/1251 nt) PP_2924 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,324,9850T. 6.1% 199.4 / 6.9 66coding (149/1251 nt)PP_2924hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (38/23);  new base . (2/2);  total (40/26)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCTGAAGCGTGCGCGCGGCACGTTGCTGGAGCGGCGGTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACTGAGTATTCA  >  NC_002947/3324841‑3325132
                                                                                                                                                |                                                                                                                                                   
gggCTGAAGCGTGCGCGCGGCACGTTGCTGGAGCGGCGGTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTtggt                                                                                                                                                 >  6:295969/1‑149 (MQ=255)
gggCTGAAGCGTGCGCGCGGCACGTTGCTGGAGCGGCGGTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTtggt                                                                                                                                                 >  2:258354/1‑149 (MQ=255)
      aaGCGTGCGCGCGGCACGTTGCTGGAGCGGCGGTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCa                                                                                                                                             <  3:139596/147‑1 (MQ=255)
          gTGCGCGCGGCACGTTGCTGGAGCGGCGGTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAgg                                                                                                                                       >  6:146077/1‑149 (MQ=255)
                 cgGCACGTTGCTGGAGCGGCGGTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAg                                                                                                                                <  7:348511/149‑1 (MQ=255)
                                gcggcgGTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTgc                                                                                                                 >  1:296274/1‑149 (MQ=255)
                                 cggcggTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCG‑TGGTGGCACCCAGGCCGTCCGCGCCGCGCGAAATtcgc                                                                                                               >  4:48409/1‑149 (MQ=255)
                                 cggcggTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCG‑TGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTgcgc                                                                                                               >  4:54015/1‑149 (MQ=255)
                                  ggcggTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTgcgc                                                                                                               >  2:185537/1‑149 (MQ=255)
                                     ggTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTg                                                                                                            >  6:265755/1‑149 (MQ=255)
                                            gCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTtgg                                                                                                                                                  <  1:430811/104‑1 (MQ=255)
                                            gCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTtgg                                                                                                                                                  >  2:430811/1‑104 (MQ=255)
                                               gTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGcaccac                                                                                                  <  2:376834/149‑1 (MQ=255)
                                                tACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCAct                                                                                                 >  1:386065/1‑149 (MQ=255)
                                                     gCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTgg                                                                                            >  8:148801/1‑149 (MQ=255)
                                                     gCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTgg                                                                                            >  6:414397/1‑149 (MQ=255)
                                                      cAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGgc                                                                                           <  1:258354/149‑1 (MQ=255)
                                                      cAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGgc                                                                                           <  5:295969/149‑1 (MQ=255)
                                                           gTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCagg                                                                                      <  3:95627/149‑1 (MQ=255)
                                                               aGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCaggagac                                                                                  <  5:146077/149‑3 (MQ=255)
                                                                 gCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGcgt                                                                                >  4:184022/1‑149 (MQ=255)
                                                                 gCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGcgt                                                                                >  5:36068/1‑149 (MQ=255)
                                                                         tGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACtt                                                                         <  5:44282/148‑1 (MQ=255)
                                                                            tCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGACAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTggcg                                                                     >  8:130822/1‑149 (MQ=255)
                                                                              agcagTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGATTTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTggcggc                                                                   >  1:310350/1‑149 (MQ=255)
                                                                              agcagTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTggcggc                                                                   <  2:386065/149‑1 (MQ=255)
                                                                                  gTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCg                                                               <  3:184022/149‑1 (MQ=255)
                                                                                      cTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGc                                                             >  7:266416/1‑147 (MQ=255)
                                                                                      cTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGc                                                             >  7:193116/1‑147 (MQ=255)
                                                                                       ttGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGcg                                                                                 >  3:176993/1‑126 (MQ=255)
                                                                                       ttGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGcg                                                                                 <  4:176993/126‑1 (MQ=255)
                                                                                         gAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAgg                                                         <  7:64869/148‑1 (MQ=255)
                                                                                           gCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCTGCAAGGATg                                                      >  6:350032/1‑149 (MQ=255)
                                                                                                   aCGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGttt                                              >  6:208665/1‑149 (MQ=255)
                                                                                                     gccgCGGCCGTCCGGGGAATAGCTGTGACAGATCCCCATGCCG‑TGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGc                                           <  3:48409/146‑1 (MQ=255)
                                                                                                     gCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTgg                                            >  4:359959/1‑149 (MQ=255)
                                                                                                     gCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTgg                                            <  7:148801/149‑1 (MQ=255)
                                                                                                     gCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTgg                                            >  2:221875/1‑149 (MQ=255)
                                                                                                     gCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTgg                                            <  5:265755/149‑1 (MQ=255)
                                                                                                     gCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCG‑TGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGc                                           <  3:54015/149‑1 (MQ=255)
                                                                                                      cAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGc                                           >  8:60006/1‑149 (MQ=255)
                                                                                                       aGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGc                                           >  5:181293/1‑148 (MQ=255)
                                                                                                         cGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGAt                                        >  3:184907/1‑149 (MQ=255)
                                                                                                                       ataGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCGTGGGCGCCCCACGGCTGGCGCAGGAGGCGGCGGACGTGGCGGCGGCGGCAAGGAGGCCGAGTTTGGCGATGAGGTGCAgggtgg                          >  8:270642/1‑149 (MQ=255)
                                                                                                                       ataGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCCCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCCTCGTACTTAGCGGCGTCGGCAAGCATGACGAGTATCGCGATGAGTTGCAtggtg                           >  4:408169/1‑148 (MQ=255)
                                                                                                                           cTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGa                      >  1:413147/1‑149 (MQ=255)
                                                                                                                            tgtgACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGAc                     >  6:86517/1‑149 (MQ=255)
                                                                                                                              tgACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGAc                     <  2:310350/147‑1 (MQ=255)
                                                                                                                              tgACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGAc                     <  1:221875/147‑1 (MQ=255)
                                                                                                                              tgACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGAc                     <  3:359959/147‑1 (MQ=255)
                                                                                                                                aCAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTg                  <  8:266416/148‑1 (MQ=255)
                                                                                                                                  aGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGa               >  2:259615/1‑149 (MQ=255)
                                                                                                                                  aGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGa               >  1:381038/1‑149 (MQ=255)
                                                                                                                                    aTACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATa             >  7:69932/1‑149 (MQ=255)
                                                                                                                                      aCCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCAGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTa                                                                            <  2:370557/84‑1 (MQ=255)
                                                                                                                                      aCCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCAGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTa                                                                            >  1:370557/1‑84 (MQ=255)
                                                                                                                                        ccATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACt           >  2:389697/1‑147 (MQ=255)
                                                                                                                                        ccATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACt           >  3:113375/1‑147 (MQ=255)
                                                                                                                                        ccATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACt           >  7:70852/1‑147 (MQ=255)
                                                                                                                                        ccATGCCGGTGGGGGCACCCAGGCCGGCAGCGCCGCGCGAATGGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACTGa         <  7:270642/149‑1 (MQ=255)
                                                                                                                                         cATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGttt                                              >  1:161285/1‑111 (MQ=255)
                                                                                                                                         cATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGttt                                              <  2:161285/111‑1 (MQ=255)
                                                                                                                                         cATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACTg          >  5:26024/1‑147 (MQ=255)
                                                                                                                                         cATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACTg          <  6:26024/147‑1 (MQ=255)
                                                                                                                                           tGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACTGAGTa      <  2:296274/149‑1 (MQ=255)
                                                                                                                                               gTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACTGAGTATTCa  >  5:360650/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GGGCTGAAGCGTGCGCGCGGCACGTTGCTGGAGCGGCGGTTGACGCAGTACTGGCAGTCGTACAGGCAGAAGTTGGTCAGCAGTACCTTGAGCAGTGATACGCAGCGGCCGTCCGGGGTATAGCTGTGACAGATACCCATGCCGTTGGTGGCACCCAGGCCGTCAGCGCCGCGCGAATTGCGCTTGGGCGCACCACTGCTGGCGCAGGAGGCGTCGTACTTGGCGGCGTCGGCAAGGATGCCGAGTTTGGCGATGAGTTGCATGGTGGCTGACCTGCGATACTGAGTATTCA  >  NC_002947/3324841‑3325132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑