Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	396,862:1	+G	7.3%	coding (84/924 nt)	PP_0329 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	396,862	1	.	G	7.3%	317.2 / 18.6	82	coding (84/924 nt)	PP_0329	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (36/40);  new base G (4/2);  total (40/42)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCACTACGATTATCT  >  NC_002947/396714‑397008                                                                                                                                                       |                                                                                                                                                   cAAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGAtt                                                                                                                                                     <  1:229788/149‑1 (MQ=255)  aaaGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGAtt                                                                                                                                                     >  8:392804/1‑148 (MQ=255)  aaaGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGAtt                                                                                                                                                     >  3:111535/1‑148 (MQ=255)  aaaGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGAtt                                                                                                                                                     >  5:425594/1‑148 (MQ=255)       gggTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGc                                                                                                                                              <  7:391373/149‑1 (MQ=255)         gtgtAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTT‑CAATCCTGCGGATTGGAACGCtc                                                                                                                                            <  1:176971/149‑2 (MQ=255)          tgtAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTc                                                                                                                                           <  6:269855/149‑1 (MQ=255)                    aTTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGttt                                                                                                                                  <  3:115837/148‑1 (MQ=255)                      ttttttGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTcc                                                                                                                                >  5:105844/1‑148 (MQ=255)                        ttttGCTTTCCGTCAGGCGTTCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGTAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCaa                                                                                                                              <  6:187997/148‑1 (MQ=255)                         tttGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAAt                                                                                                                             <  3:30405/148‑1 (MQ=255)                           tGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                           <  6:425594/148‑1 (MQ=255)                           tGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                           >  5:251835/1‑148 (MQ=255)                            gCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                           >  3:400383/1‑147 (MQ=255)                            gCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCAAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                           >  2:237210/1‑147 (MQ=255)                             cTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGTCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                           >  1:119751/1‑147 (MQ=255)                             cTTTCCGTCAGGCGTGCTAACGACAAGCACTGAGTCCATCTCATCTGTTGCTAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCTTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACa                                                                                                                         <  2:352560/148‑1 (MQ=255)                              tttCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGt                                                                                                                       >  2:438044/1‑149 (MQ=255)                               ttCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTg                                                                                                                      >  5:325310/1‑149 (MQ=255)                                tCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGACCTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTg                                                                                                                      <  1:30587/148‑1 (MQ=255)                                   gTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCa                                                                                                                  <  5:157267/149‑1 (MQ=255)                                        gCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTg                                                                                                             >  3:339231/1‑149 (MQ=255)                                         cGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGc                                                                                                            <  7:392804/149‑1 (MQ=255)                                           tGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctg                                                                                                          <  2:119751/149‑1 (MQ=255)                                              tCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTTGTCAGTTTGctggtc                                                                                                       >  4:262728/1‑149 (MQ=255)                                              tCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtc                                                                                                       >  2:379175/1‑149 (MQ=255)                                              tCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtc                                                                                                       >  5:64892/1‑149 (MQ=255)                                                aCGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctg                                                                                                     <  6:251835/149‑1 (MQ=255)                                                   aCAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctgg                                                                                                    >  5:387002/1‑147 (MQ=255)                                                         aCTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAata                                                                                            >  1:435956/1‑149 (MQ=255)                                                           tGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATAtt                                                                                          >  1:153784/1‑149 (MQ=255)                                                           tGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTCGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctggt                                                                                                   >  6:176618/1‑140 (MQ=255)                                                           tGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTCGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctggt                                                                                                   <  5:176618/140‑1 (MQ=255)                                                              gTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTgg                                                                                       <  6:64892/149‑1 (MQ=255)                                                                 catctcatctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTgggg                                                                                     <  4:400383/148‑1 (MQ=255)                                                                 catctcatctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTgggg                                                                                     <  1:379175/148‑1 (MQ=255)                                                                       atctGTTGCGAAAAG‑CTGGTAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCa                                                                               <  2:322787/148‑1 (MQ=255)                                                                       atctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACa                                                                                                                         <  7:249401/106‑1 (MQ=255)                                                                       atctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACa                                                                                                                         >  8:249401/1‑106 (MQ=255)                                                                       atctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAg                                                                              >  4:71318/1‑149 (MQ=255)                                                                          tGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGAc                                                                           >  8:144608/1‑149 (MQ=255)                                                                           gTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACt                                                                          >  1:407518/1‑149 (MQ=255)                                                                            ttGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACt                                                                          <  2:435956/148‑1 (MQ=255)                                                                               cGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGttt                                                                      <  4:111535/149‑1 (MQ=255)                                                                               cGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGttt                                                                      <  6:325310/149‑1 (MQ=255)                                                                                 aaaaG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGttttt                                                                    <  6:105844/149‑1 (MQ=255)                                                                                  aaaG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTc                                                                   <  4:169878/149‑1 (MQ=255)                                                                                        tGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGa                                                              >  2:124149/1‑149 (MQ=255)                                                                                         gggAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGAc                                                             >  6:223847/1‑149 (MQ=255)                                                                                          ggAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGAc                                                             <  3:71318/148‑1 (MQ=255)                                                                                           gAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAAACCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGt                                                           >  1:102967/1‑149 (MQ=255)                                                                                            aaCTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTg                                                          >  1:424483/1‑149 (MQ=255)                                                                                               ttCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                                       >  1:414529/1‑149 (MQ=255)                                                                                               ttCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                                       >  2:69636/1‑149 (MQ=255)                                                                                                 ccTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                                       <  5:223847/147‑1 (MQ=255)                                                                                                  cTGCTTCTTTGCCAGCGGACTAAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCg                                                    >  4:3466/1‑149 (MQ=255)                                                                                                    gCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGtt                                                  >  8:70548/1‑149 (MQ=255)                                                                                                          ttGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCa                                                                                                                  >  1:268663/1‑79 (MQ=255)                                                                                                          ttGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCa                                                                                                                  <  2:268663/79‑1 (MQ=255)                                                                                                           tGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCt                                              <  4:339231/146‑1 (MQ=255)                                                                                                              ctgCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGtt                                        >  6:430617/3‑149 (MQ=255)                                                                                                               aGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTg                                       <  6:387002/149‑1 (MQ=255)                                                                                                                gCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTgg                                      <  1:438044/149‑1 (MQ=255)                                                                                                                    aCTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                                       >  5:195568/1‑128 (MQ=255)                                                                                                                    aCTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                                       <  6:195568/128‑1 (MQ=255)                                                                                                                      tCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCgg                                <  4:423302/149‑1 (MQ=255)                                                                                                                        aGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCgg                                <  3:262728/147‑1 (MQ=255)                                                                                                                         gCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGaaa                             >  4:10894/1‑149 (MQ=255)                                                                                                                          cAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGt                                                >  5:368227/1‑129 (MQ=255)                                                                                                                          cAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGt                                                <  6:368227/129‑1 (MQ=255)                                                                                                                          cAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAAt                            <  2:407518/149‑1 (MQ=255)                                                                                                                            agagGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAg                          <  2:424483/149‑1 (MQ=255)                                                                                                                             gagGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGaaa                             >  6:387164/1‑145 (MQ=255)                                                                                                                               gggAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGtt                                                  <  7:321488/122‑1 (MQ=255)                                                                                                                               gggAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGtt                                                  >  8:321488/1‑122 (MQ=255)                                                                                                                               gggAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATg                       <  2:342285/149‑1 (MQ=255)                                                                                                                                gaaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATgg                      >  3:324127/3‑149 (MQ=255)                                                                                                                                gaaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATgg                      >  7:64209/3‑149 (MQ=255)                                                                                                                                 aaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGaa                              <  3:86229/139‑1 (MQ=255)                                                                                                                                 aaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGaa                              >  4:86229/2‑140 (MQ=255)                                                                                                                                 aaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCGCCCGGTGTCTGCAGTTGGCCCCGGAAATAGATGGc                     >  8:113789/2‑149 (MQ=255)                                                                                                                                  aGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGcc                    >  2:26220/1‑149 (MQ=255)                                                                                                                                   gCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCt                   <  3:10894/149‑1 (MQ=255)                                                                                                                                          aaTCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCAc             >  2:76940/1‑148 (MQ=255)                                                                                                                                                   gATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCACTACGATTATc   <  1:237210/149‑1 (MQ=255)                                                                                                                                                    aTT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCACTACGATTATCt  <  2:102967/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                   CAAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCACTACGATTATCT  >  NC_002947/396714‑397008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑