Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,417,332:1 +A 8.2% coding (580/759 nt) PP_1239 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,417,3321.A 8.2% 278.6 / 19.0 73coding (580/759 nt)PP_1239metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base . (33/34);  new base A (2/4);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

GCTGTTGCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGGCGAAT  >  NC_002947/1417184‑1417475
                                                                                                                                                      |                                                                                                                                               
gcTGTTGCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTgcgc                                                                                                                                                  <  6:263254/149‑1 (MQ=255)
     tGCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATGGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTg                                                                                                                                            >  7:193818/1‑149 (MQ=255)
      gCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTg                                                                                                                                            <  1:134692/148‑1 (MQ=255)
      gCTGGAGCGGTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTg                                                                                                                                            <  4:135983/148‑1 (MQ=255)
        tGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTg                                                                                                                                         >  7:379653/1‑149 (MQ=255)
        tGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTg                                                                                                                                         >  1:95310/1‑149 (MQ=255)
          gAGCGTTACCTGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCGGAGGCAGCGGGTGGGTGGCATGCAT‑GGGCATTTCAACAACCACCAGGCGGGGC‑GGCTGGTGCa                                                                                                                                       >  8:296691/1‑149 (MQ=255)
          gAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTg                                                                                                                                         <  1:11408/147‑1 (MQ=255)
          gAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGC‑GCGCAGCCTGGTg                                                                                                                                         <  2:334497/147‑1 (MQ=255)
          gAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGC‑GCGCAGCCTGGTg                                                                                                                                         <  7:51466/147‑1 (MQ=255)
           aGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCAc                                                                                                                                      <  2:295246/149‑1 (MQ=255)
           aGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGC‑GCGCAGCCTGGTggtt                                                                                                                                      <  5:110975/149‑4 (MQ=255)
               ttACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGt                                                                                                                                  >  5:420098/1‑149 (MQ=255)
               ttACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGt                                                                                                                                  <  8:116337/149‑1 (MQ=255)
               ttACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGt                                                                                                                                  <  8:122480/149‑1 (MQ=255)
                  ccAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGa                                                                                                                                    >  4:424351/1‑144 (MQ=255)
                  ccAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGa                                                                                                                                    <  3:424351/144‑1 (MQ=255)
                   cAGTGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACTACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTggg                                                                                                                              <  5:292915/149‑1 (MQ=255)
                     gggCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCt                                                                                                                            <  4:221834/149‑1 (MQ=255)
                      ggCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGGTGGCAGGTGGTCACTACGCGGCGTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACTACGACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTg                                                                                                                           <  7:168610/149‑1 (MQ=255)
                         cTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGaa                                                                                                                        >  2:317656/1‑149 (MQ=255)
                            gATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAACGGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGaa                                                                                                                        <  1:114797/147‑1 (MQ=255)
                            gATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGaacaa                                                                                                                     <  2:95310/149‑1 (MQ=255)
                             aTGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGaacaac                                                                                                                    <  5:245521/149‑1 (MQ=255)
                              tGCACTGCTGATCGAGGCTAATCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAAcc                                                                                                                   >  7:76066/1‑149 (MQ=255)
                                cACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTg                                                                                                                 <  3:120682/149‑1 (MQ=255)
                                 aCTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTg                                                                                                                 <  8:345635/148‑1 (MQ=255)
                                 aCTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTg                                                                                                                 >  7:345635/1‑148 (MQ=255)
                                       gATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACAcc                                                                                                          >  2:327506/1‑149 (MQ=255)
                                        aTCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCt                                                                                                         >  5:292695/1‑149 (MQ=255)
                                         tCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTg                                                                                                        >  2:319417/1‑149 (MQ=255)
                                          cGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTg                                                                                                        <  8:379653/148‑1 (MQ=255)
                                                  aCCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGcc                                                                                               >  8:39125/1‑149 (MQ=255)
                                                  aCCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGC‑GCGCAGCCTGGTggt                                                                                                                                       <  3:422666/109‑3 (MQ=255)
                                                  aCCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGC‑GCGCAGCCTGGTggt                                                                                                                                       >  4:422666/1‑107 (MQ=255)
                                                    cACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCa                                                                                             >  2:55110/1‑149 (MQ=255)
                                                     aCTGTCGCGACCTGCTGGCAAGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGTGTGGCATGCAA‑TGGCATTTTAACAACCACCAGGCTGCGC‑GCCTGGTGAACGAGTTTGGCTGTAACAACCTGCAACACCTGGTGCTGGCCCAc                                                                                            <  6:439906/149‑1 (MQ=255)
                                                         tCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCaa                                                                                                              <  7:75715/127‑1 (MQ=255)
                                                         tCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCaa                                                                                                              >  8:75715/1‑127 (MQ=255)
                                                           gcgACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTcag                                                                                       >  3:155354/1‑148 (MQ=255)
                                                            cgACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCt                                                                                                   <  5:49725/135‑1 (MQ=255)
                                                            cgACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCt                                                                                                   >  6:49725/1‑135 (MQ=255)
                                                               ccTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAgcagca                                                                                  <  6:420098/149‑1 (MQ=255)
                                                                ctgctgGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCATCAGGCTGCGC‑GCCTGGt                                                                                                                                          >  4:389032/1‑92 (MQ=255)
                                                                ctgctgGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCATCAGGCTGCGC‑GCCTGGt                                                                                                                                          <  3:389032/92‑1 (MQ=255)
                                                                   ctgGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAg                                                                                <  3:50552/147‑1 (MQ=255)
                                                                   ctgGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGaa                                                                              <  5:135211/149‑1 (MQ=255)
                                                                      gCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGaacaa                                                                           >  1:262342/1‑149 (MQ=255)
                                                                      gCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGaac                                                                             <  1:319417/147‑1 (MQ=255)
                                                                             gTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGTCCACCTCAGCAGCAAGAACAACCTGCca                                                                    >  3:187015/1‑149 (MQ=255)
                                                                               cACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCcaca                                                                  >  1:5310/1‑149 (MQ=255)
                                                                                   aCCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTg                                                              >  1:410622/1‑149 (MQ=255)
                                                                                     ccGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGc                                                            >  1:87643/1‑149 (MQ=255)
                                                                                        gCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCg                                                         >  6:384493/1‑149 (MQ=255)
                                                                                         cTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGc                                                        <  6:292695/149‑1 (MQ=255)
                                                                                                  agcagcGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCttt                                               <  1:55110/149‑1 (MQ=255)
                                                                                                     agcGTGTGGGTGGCATGCAA‑GGGCATTTGAACAACCAGCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCGCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTc                                            <  8:81918/149‑1 (MQ=255)
                                                                                                        gggtgggtGTCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGATCAACCTGCTACACCTGGTGCTGGCCCACCTCAGCAGCTAGAACAACCTGCCAGACTTGGCGCGCCAGTGCTTTGTCGac                                         <  8:193818/149‑1 (MQ=255)
                                                                                                              gtggCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTc                                   <  2:5310/149‑1 (MQ=255)
                                                                                                                 gCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCgg                                 <  3:131730/148‑1 (MQ=255)
                                                                                                                       aa‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGAc                          >  6:388493/1‑149 (MQ=255)
                                                                                                                       aa‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGAc                          <  1:401887/149‑1 (MQ=255)
                                                                                                                              aTTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGAc                    >  1:403168/1‑149 (MQ=255)
                                                                                                                               tttGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGTACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGACCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACt                   >  1:95858/1‑149 (MQ=255)
                                                                                                                               tttGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACt                   >  7:326565/1‑149 (MQ=255)
                                                                                                                                    acaacCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCt                                                                        >  5:422259/1‑91 (MQ=255)
                                                                                                                                    acaacCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCt                                                                        <  6:422259/91‑1 (MQ=255)
                                                                                                                                     caacCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTc              >  3:104923/1‑148 (MQ=255)
                                                                                                                                       tgcaccaGGC‑GCGCAGCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAg           >  1:213/3‑149 (MQ=255)
                                                                                                                                        ccaccaGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAgg          >  6:114788/1‑149 (MQ=255)
                                                                                                                                         caccaGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGt         >  5:189421/1‑149 (MQ=255)
                                                                                                                                            caGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGGc      <  4:155354/149‑1 (MQ=255)
                                                                                                                                              ggCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGGCGa    <  7:64178/149‑1 (MQ=255)
                                                                                                                                                 tGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGGCGAAt  >  7:319708/1‑148 (MQ=255)
                                                                                                                                                      |                                                                                                                                               
GCTGTTGCTGGAGCGTTACCAGGGCCTGGATGCACTGCTGATCGAGGCTAACCACTGTCGCGACCTGCTGGCACGTGGTCACTACCCGGCTTTTCTGAAGCAGCGGGTGGGTGGCATGCAA‑GGGCATTTGAACAACCACCAGGCTGCGC‑GCCTGGTGCACGAGTTGGGCTGGAACAACCTGCAACACCTGGTGCTGGCCCACCTCAGCAGCAAGAACAACCTGCCACACTTGGCCCGCCAGTGCTTTGTCGACACCCTCGGGTGTGACCCGGACTGGCTCCAGGTGGCGAAT  >  NC_002947/1417184‑1417475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑