Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,945,621:1	+C	12.1%	coding (398/531 nt)	PP_2578 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,945,621	1	.	C	12.1%	222.4 / 28.1	66	coding (398/531 nt)	PP_2578	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (23/35);  new base C (5/3);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGCCCTGCAGGACCTGTTGGGCGGCCCGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCATGAACCTTGGCCT  >  NC_002947/2945475‑2945764                                                                                                                                                    |                                                                                                                                                cAGCGCCCTGCAGGACCTGTTGGGCGGCCCGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑Ga                                                                                                                                               <  7:209874/149‑1 (MQ=255)     gcCCTGCAGGACCTGTTGGGCGGCCCGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTg                                                                                                                                           <  4:396593/149‑1 (MQ=255)           cAGGACCTGTTGGGCGGCCCGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGt                                                                                                                                       <  5:306187/147‑1 (MQ=255)           cAGGACCTGTTGGGCGGCCCGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTAc                                                                                                                                     <  7:25782/149‑1 (MQ=255)             ggACCTGTTGGGCGGCCCGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGt                                                                                                                                   <  5:48027/149‑1 (MQ=255)              gACCTGTTGGGCGGCCCGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGt                                                                                                                                   >  6:5473/1‑148 (MQ=255)                 cTGTTGGGCGGCCCGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGt                                                                                                                               >  3:54682/1‑149 (MQ=255)                            cccGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGActgctg                                                                                                                    <  5:10377/149‑1 (MQ=255)                             ccGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACtgctgc                                                                                                                   >  5:343843/1‑149 (MQ=255)                              cggcggGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCg                                                                                                                  <  6:32921/149‑1 (MQ=255)                                gcgggcggTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGa                                                                                                                <  2:185886/149‑1 (MQ=255)                                 cgggcggTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGac                                                                                                               <  5:109260/149‑1 (MQ=255)                                   ggcggTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGacaa                                                                                                             <  7:11658/149‑1 (MQ=255)                                    gcggTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGacaac                                                                                                            <  6:343843/149‑1 (MQ=255)                                                 gTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGcc                                                                                               >  1:182584/1‑149 (MQ=255)                                                   cGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTg                                                                                             >  4:7712/1‑149 (MQ=255)                                                    gCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTgg                                                                                            >  5:16785/1‑149 (MQ=255)                                                     cTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGAGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGt                                                                                           <  1:179842/149‑1 (MQ=255)                                                        cGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGACGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGaa                                                                                        >  5:224523/1‑149 (MQ=255)                                                         gggTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAAt                                                                                       <  5:5473/149‑1 (MQ=255)                                                         gggTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAAt                                                                                       <  3:19292/149‑1 (MQ=255)                                                           gTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGACGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATgc                                                                                     <  6:224523/149‑1 (MQ=255)                                                                       cGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCaa                                                                         <  4:141001/149‑1 (MQ=255)                                                                        gCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAAc                                                                        <  2:182584/149‑1 (MQ=255)                                                                          ttCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCt                                                                      >  7:297434/1‑149 (MQ=255)                                                                             gACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGGAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTgc                                                                    <  5:440700/148‑1 (MQ=255)                                                                                 cGGTACCAGGGCGCTACGCCTCGCTGGACGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAg                                                               <  5:259325/149‑1 (MQ=255)                                                                                    tACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTg                                                            <  3:396794/149‑1 (MQ=255)                                                                                      ccAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTgg                                                           <  3:33014/148‑1 (MQ=255)                                                                                        aGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGcc                                                         >  6:154958/1‑148 (MQ=255)                                                                                         gggCGCTACTCCTCGCGGGAGGCCGTGGGGCGCCTGAAGCAGCAAGGGCAGGGCGAGCT‑GACCTGCCGGACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACTTGCGCAGGTTGGcctg                                                       <  4:15919/149‑3 (MQ=255)                                                                                                    cTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCggg                                            >  3:178508/1‑149 (MQ=255)                                                                                                    cTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCggg                                            >  7:26832/1‑149 (MQ=255)                                                                                                    cTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCggg                                            >  2:145759/1‑149 (MQ=255)                                                                                                    cTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAACTTGGCCGAGGCGGTGCcgg                                            >  8:208435/1‑149 (MQ=255)                                                                                                     tCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCgggg                                           >  8:101634/1‑149 (MQ=255)                                                                                                       gCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGa                                         <  7:101634/149‑1 (MQ=255)                                                                                                         tGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGt                                       <  8:297434/149‑1 (MQ=255)                                                                                                            aGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GATCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGcc                                    <  1:145759/149‑1 (MQ=255)                                                                                                            aGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGcc                                    >  3:77852/1‑149 (MQ=255)                                                                                                              gCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGTGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCg                                  <  5:246720/149‑1 (MQ=255)                                                                                                                     ggCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAggg                            >  8:349870/1‑148 (MQ=255)                                                                                                                      gcgcCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAggg                            >  1:257131/1‑147 (MQ=255)                                                                                                                      gcgcCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTa                          <  4:77852/149‑1 (MQ=255)                                                                                                                       cgcCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCCAGGCGGTGCGGGGAAGTCCCCGGCAGGGTAg                         >  3:356730/1‑149 (MQ=255)                                                                                                                        gcCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGc                        <  3:7712/149‑1 (MQ=255)                                                                                                                            gAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGcc                    >  3:292892/1‑149 (MQ=255)                                                                                                                             aaGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGc                     <  7:349870/147‑1 (MQ=255)                                                                                                                              agcagcAAGGGCACGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCGGGTGAATGCGCACCAGGCCAACCTGCGCAAGTGGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCt                  <  3:334916/149‑1 (MQ=255)                                                                                                                                cagcaAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGc                <  2:257131/149‑1 (MQ=255)                                                                                                                                cagcaAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGCCCGAGGCGGTGCGGGCGAGTGCCCGGCAGGGTAGCTGCCCTg                 >  5:228869/1‑148 (MQ=255)                                                                                                                                 agcaAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGATAGCTGCCCTGCa               <  6:16785/149‑1 (MQ=255)                                                                                                                                  gcaAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCAt              >  1:21640/1‑149 (MQ=255)                                                                                                                                   gtacggCAGGTC‑AGCTCGACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGt                           >  3:156802/5‑135 (MQ=255)                                                                                                                                   gtacggCAGGTC‑AGCTCGACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGt                           <  4:156802/131‑1 (MQ=255)                                                                                                                                      cggCAGGTC‑AGCTCGACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGc                <  2:116616/142‑1 (MQ=255)                                                                                                                                      cggCAGGTC‑AGCTCGACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGc                >  1:116616/2‑143 (MQ=255)                                                                                                                                      cggCAGGTC‑AGCTCGACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCa               <  7:184329/143‑1 (MQ=255)                                                                                                                                      cggCAGGTC‑AGCTCGACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCa               >  8:184329/2‑144 (MQ=255)                                                                                                                                      cggCAGGTC‑AGCTCGACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCATGAAc          >  1:90542/2‑149 (MQ=255)                                                                                                                                       ggCAGGTC‑AGCTCGACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCATGAAcc         >  7:134135/1‑149 (MQ=255)                                                                                                                                           ggTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCATGAACCTTgg     >  8:251313/1‑149 (MQ=255)                                                                                                                                            gTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCATGAACCTTGGc    <  2:21640/149‑1 (MQ=255)                                                                                                                                               gAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTg                                                                                             >  7:258308/1‑57 (MQ=255)                                                                                                                                               gAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTg                                                                                             <  8:258308/57‑1 (MQ=255)                                                                                                                                               gAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCATGAACCTTGGCCt  <  4:292892/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                CAGCGCCCTGCAGGACCTGTTGGGCGGCCCGGCGGGCGGTGGCAAGGCGTCGCTGCGGGTGGATGTGCAACGCTTCGACACGGTACCAGGGCGCTACGCCTCGCTGGAGGCCGTGTGGCGCCTGAAGCAGCAAGGGCAGGTCGAGCT‑GACCTGCCGTACGTCAGTGCAGACTGCTGCGGACAACAGCATCACCAGCCTGGTGAATGCGCACCAGGCCAACCTGCGCAAGTTGGCCGAGGCGGTGCGGGGGAGTGCCCGGCAGGGTAGCTGCCCTGCATGAACCTTGGCCT  >  NC_002947/2945475‑2945764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑