Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,447,838:1	+T	6.8%	intergenic (‑274/‑48)	nicC ← / → nicX	6‑hydroxynicotinate 3‑monooxygenase/2,5‑dihydroxypyridine 5,6‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,447,838	1	.	T	6.8%	234.1 / 9.7	59	intergenic (‑274/‑48)	nicC/nicX	6‑hydroxynicotinate 3‑monooxygenase/2,5‑dihydroxypyridine 5,6‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base . (25/30);  new base T (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGAAATCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCAC  >  NC_002947/4447690‑4447984                                                                                                                                                      |                                                                                                                                                     acgaaaTCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCa                                                                                                                                                       <  1:212711/149‑1 (MQ=255) acgaaaTCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCa                                                                                                                                                       <  3:128580/149‑1 (MQ=255)    aaaTCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGc                                                                                                                                                   <  1:243470/149‑1 (MQ=255)      aTCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCAc                                                                                                                                                 <  5:422451/149‑1 (MQ=255)             tGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATg                                                                                                                                          >  2:351569/1‑149 (MQ=255)               aTTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGt                                                                                                                                        >  3:396886/1‑149 (MQ=255)                ttGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACTTTTGAACAGATCACTTGCGCTCTCTTGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACAATTATTTTGCACAGAGATGCAGCACCATGGCAGTGCC‑TGGTg                                                                                                                                       <  4:415423/149‑1 (MQ=255)                  gTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCATGGCAGTGCC‑TGGTgct                                                                                                                                     >  1:53321/1‑148 (MQ=255)                   ttACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCATGGCAGTGCC‑TGGTgctg                                                                                                                                    <  8:303497/149‑3 (MQ=255)                     aCCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGcc                                                                                                                                   <  6:360903/148‑1 (MQ=255)                      ccGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTgc                                                                                                                                      <  1:106133/144‑1 (MQ=255)                      ccGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTgc                                                                                                                                      >  2:106133/1‑144 (MQ=255)                      ccGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGccc                                                                                                                                  <  3:77602/148‑1 (MQ=255)                         gaaaTACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGGACAAGTAGGGTGTACACACTATTATTTTGCACCGAGCTGCAGCACCA‑GGCACTGCCATGGTGCGcccgggt                                                                                                                              >  8:127445/2‑145 (MQ=255)                               cTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGc                                                                                                                        <  5:274385/149‑1 (MQ=255)                                tGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGc                                                                                                                        >  4:415221/1‑148 (MQ=255)                                      aTGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAg                                                                                                                 <  5:335900/149‑1 (MQ=255)                                       tgtgtgGATAGGTAACGTTTGCACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCa                                                                                                                  <  8:185884/147‑1 (MQ=255)                                       tgtgtgGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAgg                                                                                                                <  7:20679/149‑1 (MQ=255)                                         tgtgGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGga                                                                                                               <  7:330294/148‑1 (MQ=255)                                         tgtgGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGga                                                                                                               <  5:327399/148‑1 (MQ=255)                                           tgGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGaa                                                                                                            <  6:196879/149‑1 (MQ=255)                                                  gTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑At                                                                                                   <  4:396886/149‑1 (MQ=255)                                                           gAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTACGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGa                                                                                           <  2:66325/148‑1 (MQ=255)                                                            aaCAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAg                                                                                          >  8:277108/1‑148 (MQ=255)                                                            aaCAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGGGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGc                                                                                         >  8:309325/1‑149 (MQ=255)                                                                  tCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCGCCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACACCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGca                                                                                   >  2:125272/1‑149 (MQ=255)                                                                  tCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGca                                                                                   <  1:351569/149‑1 (MQ=255)                                                                    aCTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGAACCA‑GGCACTGCAATGGTGCGCCCCTGTCAACTCCACCAAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGcac                                                                                  >  1:388128/1‑148 (MQ=255)                                                                            tcgctGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGc                                                                                                 >  1:83189/1‑125 (MQ=255)                                                                            tcgctGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGc                                                                                                 <  2:83189/125‑1 (MQ=255)                                                                            tcgctGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGAcc                                                                          >  7:106887/1‑148 (MQ=255)                                                                              gctGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGAccc                                                                         <  1:265480/147‑1 (MQ=255)                                                                                    aGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTc                                                                 <  8:106887/149‑1 (MQ=255)                                                                                      gATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTTCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCATATGTTCGa                                                               <  8:218844/149‑1 (MQ=255)                                                                                          ccttttCATCTGCACAAGTAGGGTGTACACACAATTATTTTGCACCGAGATTCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCGGG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCa                                                            <  4:42016/146‑1 (MQ=255)                                                                                            ttttCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACg                                                          >  1:295635/1‑148 (MQ=255)                                                                                                 aTCTGCACAAGTAGGGTGTACCCACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTg                                                     >  5:155947/1‑148 (MQ=255)                                                                                                 aTCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGTCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTg                                                     >  7:219604/1‑148 (MQ=255)                                                                                                  tCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTg                                                     >  8:212173/1‑147 (MQ=255)                                                                                                    tGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGc                                                 >  7:46580/1‑149 (MQ=255)                                                                                                      cacaAGTCGGGGGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GTCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGaa                                                   <  1:346567/145‑1 (MQ=255)                                                                                                         aaGTAGGGTGTACACACTATTATTTTGCTCCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGtct                                            >  1:26433/1‑149 (MQ=255)                                                                                                         aaGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGa                                                                                           >  4:302266/1‑102 (MQ=255)                                                                                                         aaGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGa                                                                                           <  3:302266/102‑1 (MQ=255)                                                                                                                gtgtACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGTGAACCCCGCCATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGt                                       <  1:125632/149‑1 (MQ=255)                                                                                                                gtgtACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTgg                                     <  7:277108/149‑1 (MQ=255)                                                                                                                 tgtACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGa                                    <  8:46580/149‑1 (MQ=255)                                                                                                                  gtACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGAc                                   >  1:69919/1‑149 (MQ=255)                                                                                                                      acacTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGTCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGaaa                               <  8:219604/149‑1 (MQ=255)                                                                                                                       aacTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAAc                              <  7:127445/148‑1 (MQ=255)                                                                                                                        acTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACg                             >  5:251159/1‑149 (MQ=255)                                                                                                                                     aCCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGt                                       >  7:355165/1‑126 (MQ=255)                                                                                                                                     aCCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGt                                       <  8:355165/126‑1 (MQ=255)                                                                                                                                       cGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGa                                    >  4:143110/1‑127 (MQ=255)                                                                                                                                       cGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGa                                    <  3:143110/127‑1 (MQ=255)                                                                                                                                       cGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTg              >  6:333858/1‑149 (MQ=255)                                                                                                                                         agaTGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCt            >  4:104919/1‑149 (MQ=255)                                                                                                                                            tGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTc           >  7:150929/1‑147 (MQ=255)                                                                                                                                             ggcgcaCCATGGCAGTGCC‑TGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTc           >  6:418036/4‑146 (MQ=255)                                                                                                                                                   ccA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCac  <  3:415221/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     ACGAAATCCGGTTGATTGTTACCGTAAATACTGGTCAATGTGTGGATAGGTAACGTTTGAACAGATCACTTGCGCTCGCTGGCAGGATGCGTTTTCATCTGCACAAGTAGGGTGTACACACTATTATTTTGCACCGAGATGCAGCACCA‑GGCACTGCCATGGTGCGCCCCTGTCAACGCCACCCAGGAGAACCCCG‑‑ATGCCGGTGAGCAATGCACAACTGACCCAGATGTTCGAGCACGTACTGAAGCTGTCTCGAGTGGACGAAACGCAGAGCGTGGCCGTGCTCAAGAGCCAC  >  NC_002947/4447690‑4447984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑