Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 337,825 G→T 31.3% intergenic (‑75/+54) PP_0279 ← / ← PP_0280 hypothetical protein/amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947337,8250GT31.3% 77.2 / 40.1 48intergenic (‑75/+54)PP_0279/PP_0280hypothetical protein/amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (22/11);  new base T (9/6);  total (31/17)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

GATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACA  >  NC_002947/337685‑337967
                                                                                                                                             |                                                                                                                                              
gATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGgcgc                                                                                                                                        <  1:166848/149‑1 (MQ=255)
gATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGgcgc                                                                                                                                        >  2:166848/1‑149 (MQ=255)
        aCTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCtg                                                                                                                                >  8:345566/1‑149 (MQ=255)
        aCTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGGGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCtg                                                                                                                                <  5:282011/149‑1 (MQ=25)
         cTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCtgt                                                                                                                               <  8:149892/149‑1 (MQ=25)
         cTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCtgt                                                                                                                               <  7:227638/149‑1 (MQ=25)
         cTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCCCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCtgt                                                                                                                               <  4:122415/149‑1 (MQ=255)
          tGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTgg                                                                                                                                           <  4:321908/136‑1 (MQ=255)
          tGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTgg                                                                                                                                           >  3:321908/1‑136 (MQ=255)
            gTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTgg                                                                                                                             >  4:294138/1‑148 (MQ=255)
                aGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGGGGAGGGGGTTGGGCATGGCAAGGATGTGTGGTGGGTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGGTGGCGCTACACCTGTGGGAGCg                                                                                                                        <  5:320807/149‑1 (MQ=255)
                 gggCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCtg                                                                                                                                >  6:343150/1‑140 (MQ=25)
                 gggCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCtg                                                                                                                                <  5:343150/140‑1 (MQ=25)
                 gggCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCgg                                                                                                                       <  3:294138/149‑1 (MQ=255)
                 gggCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCgg                                                                                                                       <  7:345566/149‑1 (MQ=255)
                  ggCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCgg                                                                                                                       <  6:249845/148‑1 (MQ=21)
                   gCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGc                                                                                                                     <  1:398920/149‑1 (MQ=255)
                                  cTTAGGTGGGGGCTGTGGAGGGGGGTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCgg                                                                                                                       <  1:433821/132‑1 (MQ=255)
                                                                                                          aCGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCt                                                     >  6:197522/1‑126 (MQ=255)
                                                                                                          aCGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCt                                                     <  5:197522/126‑1 (MQ=255)
                                                                                                            gCCCGCTCCCACAGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAg                            >  2:357669/1‑149 (MQ=21)
                                                                                                            gCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGTCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAg                            >  6:261434/1‑149 (MQ=255)
                                                                                                            gCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAg                            >  2:101858/1‑149 (MQ=255)
                                                                                                              ccGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCa                          >  2:329821/1‑149 (MQ=255)
                                                                                                              ccGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCa                          >  2:342145/1‑149 (MQ=255)
                                                                                                                  tCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGaa                      >  1:112729/1‑149 (MQ=255)
                                                                                                                  tCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGaa                      >  7:157063/1‑149 (MQ=255)
                                                                                                                    ccACAGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  1:256687/1‑148 (MQ=25)
                                                                                                                    ccACAGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  7:243416/1‑148 (MQ=25)
                                                                                                                    ccACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  4:430460/1‑148 (MQ=255)
                                                                                                                    ccACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  5:249312/1‑148 (MQ=255)
                                                                                                                    ccACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     <  3:430460/148‑1 (MQ=255)
                                                                                                                     cacaGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCCGCATGAAc                     >  8:98789/1‑147 (MQ=21)
                                                                                                                     cacaGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  3:209682/1‑147 (MQ=255)
                                                                                                                      acaGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  6:132693/1‑146 (MQ=25)
                                                                                                                      acaGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  3:350737/1‑146 (MQ=25)
                                                                                                                      acaGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  5:122657/1‑146 (MQ=25)
                                                                                                                      acaGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     <  4:350737/146‑1 (MQ=25)
                                                                                                                       caGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  8:55332/1‑145 (MQ=255)
                                                                                                                        aGGTGTAGCGCCAACCTTGAATGTGGCGCTATACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  3:307449/1‑144 (MQ=25)
                                                                                                                        aGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  6:195599/1‑144 (MQ=255)
                                                                                                                        aGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAAc                     >  7:70262/1‑144 (MQ=255)
                                                                                                                                  ccACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAgg         >  2:6271/1‑146 (MQ=255)
                                                                                                                                  ccACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAgg         >  7:164834/1‑146 (MQ=255)
                                                                                                                                  ccACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAgg         >  4:37149/1‑146 (MQ=255)
                                                                                                                                  ccACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAg      <  8:70262/149‑1 (MQ=255)
                                                                                                                                     acaTTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGaa    >  6:435316/1‑149 (MQ=255)
                                                                                                                                       aTTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAaca  >  5:355391/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                              
GATCAGGAACTGGTCGAGGGCCTGGAAGCGGGTGCTTAGGTGGGGGCTGTGGAGGTGGTTGGGCATGGCAAGGATGTGTGGTGGTTTTGAGGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTATAGCGCCACATTCAAGGTTGGCGCTACACCTGTGGGAGCGGGCGTGCCCGCGAAGCAGTCAGTGAGGTCTCAGCGGCCTTGGCAGGCATCCACGCGCAACCAGCGCTCCAGCAGCTTGAAGCCTTGCACCAGCATGAACGAGATCACCAGGTAGAACA  >  NC_002947/337685‑337967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: