Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,711,822	C→G	6.2%	L18L (CTC→CTG) ‡	PP_4171 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,711,822	0	C	G	6.2%	248.0 / 4.7	78	L18L (CTC→CTG) ‡	PP_4171	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/30);  new base G (3/2);  total (46/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCGGTGCCC  >  NC_002947/4711679‑4711970                                                                                                                                                |                                                                                                                                                      tACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggc                                                                                                                                                    >  6:202520/1‑147 (MQ=255) tACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggc                                                                                                                                                    <  5:202520/147‑1 (MQ=255)    ttttCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCa                                                                                                                                               <  8:22998/149‑1 (MQ=255)          aGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTc                                                                                                                                                >  6:292882/1‑142 (MQ=255)          aGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTc                                                                                                                                                <  5:292882/142‑1 (MQ=255)           gACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTg                                                                                                                                        >  1:346933/1‑149 (MQ=255)                    ccGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCgacg‑ac                                                                                                                              >  4:196792/1‑149 (MQ=255)                       acacCCACTAGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTg                                                                                                                           >  6:298386/1‑149 (MQ=255)                         acCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGTCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggcgg                                                                                                                                                  >  6:139708/1‑125 (MQ=255)                         acCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGTCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggcgg                                                                                                                                                  <  5:139708/125‑1 (MQ=255)                         acCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGt                                                                                                                          >  4:369245/1‑148 (MQ=255)                           ccACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCa                                                                                                                       <  6:59192/149‑1 (MQ=255)                            cACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAg                                                                                                                                              >  2:298371/1‑126 (MQ=255)                            cACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCAt                                                                                                                      <  2:207954/149‑1 (MQ=255)                               tGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTCa                                                                                                                                               >  4:247331/1‑122 (MQ=255)                               tGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTCa                                                                                                                                               <  3:247331/122‑1 (MQ=255)                                   aaaaCCCCCACGGTGAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaac                                                                                                               <  2:7969/149‑1 (MQ=255)                                   aaaaCCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaa                                                                                                                >  5:203107/1‑148 (MQ=255)                                   aaaaCCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaa                                                                                                                >  4:58028/1‑148 (MQ=255)                                   aaaaCCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaa                                                                                                                >  8:99689/1‑148 (MQ=255)                                      acacccACGGTCATTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCTGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACAcc                                                                                                            <  6:241190/146‑1 (MQ=255)                                       cccccACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACAcc                                                                                                            >  1:123133/1‑148 (MQ=255)                                         cccACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGAc                                                                                                         <  5:196908/149‑1 (MQ=255)                                               gTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCca                                                                                                   <  3:195609/149‑1 (MQ=255)                                                    ttACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGt                                                                                              >  5:166227/1‑149 (MQ=255)                                                      aCCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGtt                                                                                             <  3:196792/148‑1 (MQ=255)                                                       ccATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTc                                                                                            <  2:289500/148‑1 (MQ=255)                                                         aTGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGa                                                                                         >  8:69802/1‑149 (MQ=255)                                                         aTGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGa                                                                                         <  6:393854/149‑1 (MQ=255)                                                         aTGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCGGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGa                                                                                         >  2:254599/1‑149 (MQ=255)                                                            ccTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCg                                                                                      <  5:298386/149‑1 (MQ=255)                                                              tGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGc                                                                                     >  6:436523/1‑148 (MQ=255)                                                                 ttCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑CCCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCcaca                                                                                 >  2:278563/1‑149 (MQ=255)                                                                 ttCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCcaca                                                                                 >  1:427570/1‑149 (MQ=255)                                                                   cAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGc                                                                                     >  7:361292/1‑143 (MQ=255)                                                                   cAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGc                                                                                     <  8:361292/143‑1 (MQ=255)                                                                   cAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCAACGACAACACCGACCTGCCACAGGTTCTGACCGCCACACg                                                                               >  1:200013/1‑149 (MQ=255)                                                                    aaTCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCacaccc                                                                              <  7:99689/149‑3 (MQ=255)                                                                      tCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGGGGTCAGGCCGTGGCCGACGAAGAAGTTAATCCACAACACCGACAGGCCACAGGTTATGCCCCCCCCACGcc                                                                             >  1:142466/1‑149 (MQ=255)                                                                      tCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCt                                                                            >  4:214831/1‑149 (MQ=255)                                                                      tCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCt                                                                            >  8:75864/1‑149 (MQ=255)                                                                        cTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTg                                                                           >  6:169055/1‑148 (MQ=255)                                                                        cTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTg                                                                           <  5:169055/148‑1 (MQ=255)                                                                             aCAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGCCAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACaa                                                                     <  1:315308/149‑1 (MQ=255)                                                                             aCAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACaa                                                                     <  1:204820/149‑1 (MQ=255)                                                                               aaaGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATc                                                                   <  1:118318/149‑1 (MQ=255)                                                                                 aGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATccc                                                                 >  8:49680/1‑149 (MQ=255)                                                                                  ggCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATcccc                                                                >  2:370203/1‑149 (MQ=255)                                                                                     cGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCccgc                                                             >  1:9444/1‑149 (MQ=255)                                                                                          cGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTg                                                        <  3:403702/149‑1 (MQ=255)                                                                                            tgtTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTc                                                      <  2:346933/149‑1 (MQ=255)                                                                                             gtTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTcc                                                     >  5:75938/1‑149 (MQ=255)                                                                                                 ccTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGCCCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCggg                                                 >  4:408926/1‑149 (MQ=255)                                                                                                  cTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCggg                                                 >  8:51779/1‑148 (MQ=255)                                                                                                   tGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCggg                                                 >  1:351824/1‑147 (MQ=255)                                                                                                     gCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGgcag                                              <  5:436523/148‑1 (MQ=255)                                                                                                     gCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGgca                                               <  6:203107/147‑1 (MQ=255)                                                                                                           ccccGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGAcc                                       >  6:386799/1‑149 (MQ=255)                                                                                                             ccGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGt                                     <  7:51779/149‑1 (MQ=255)                                                                                                               gTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGCCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTAc                                   <  7:319260/149‑1 (MQ=255)                                                                                                                tACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACt                                  <  2:9444/149‑1 (MQ=255)                                                                                                                 aCCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTc                                 >  6:65547/1‑149 (MQ=255)                                                                                                                    cgcgCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGa                               >  6:409390/1‑148 (MQ=255)                                                                                                                     gcgcCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACa                             <  1:370203/149‑1 (MQ=255)                                                                                                                        ccTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGc                           >  4:169903/1‑148 (MQ=255)                                                                                                                         ctgctgTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCg                          >  8:351107/1‑148 (MQ=255)                                                                                                                         ctgctgTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCg                          >  7:247494/1‑148 (MQ=255)                                                                                                                          tgctgTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGaa                        >  4:79714/1‑149 (MQ=255)                                                                                                                            cacggcctGACCGCCGAGCAGGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACt                      >  4:217317/8‑149 (MQ=255)                                                                                                                              gTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGa                    >  8:406577/1‑149 (MQ=255)                                                                                                                               ttATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTg                     <  3:214831/147‑1 (MQ=255)                                                                                                                                 aTTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGAtt                  <  3:369245/148‑1 (MQ=255)                                                                                                                                  ttGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACACGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTc                 <  8:247494/148‑1 (MQ=255)                                                                                                                                  ttGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACAGACCTCCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCTCTGTCCAGCGCAGCATGACCGTACTCGACAGCGAACTGATTcg                >  2:54720/1‑149 (MQ=255)                                                                                                                                    gACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGCCCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGACCTGATTcgcg              >  7:167554/1‑149 (MQ=255)                                                                                                                                    gACCGCCCTGCTCGGAGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTcgcg              >  4:337984/1‑149 (MQ=255)                                                                                                                                     aCCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCTGCATGACCGTACTCGACAGCGAACTGATTCgcgc             <  7:406577/149‑1 (MQ=255)                                                                                                                                     aCCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCgcgc             >  7:158164/1‑149 (MQ=255)                                                                                                                                          ccTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCg        <  1:254599/149‑1 (MQ=255)                                                                                                                                             gCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCGGTg     >  6:101960/1‑149 (MQ=255)                                                                                                                                              cTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCACGCCAGCGGTGc    >  1:318463/1‑149 (MQ=255)                                                                                                                                               tCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCGGTGcc   >  6:396040/1‑149 (MQ=255)                                                                                                                                                cggcggTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCGGTGccc  >  1:7302/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                      TACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCGGTGCCC  >  NC_002947/4711679‑4711970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑